Immunocytochemistry (ICC)
(Immunocytochemistry staining of acetone/methanol fixed bovine peripheral blood (lymphocyte-enriched suspension) using anti-bovine CD18 (IVA35); secondary antibody conjugated to FITC (green), DNA stained with DAPI (blue).)
Mouse anti-Cow CD18 Antibody | anti-CD18 antibody
Anti-Bov CD18 Purified
Gene Names
ITGB2; CD18
Reactivity
Cow
Applications
Flow Cytometry, Functional Assay, Immunoprecipitation, Immunocytochemistry
Purity
Purified by Protein-A Affinity Chromatography
Synonyms
CD18; Anti-Bov CD18 Purified; integrin subunit beta 2; Integrin beta2; Complement receptor C3 subunit beta; ITGB2; anti-CD18 antibody
Host
Mouse
Reactivity
Cow
Isotype
IgG1
Clone Number
IVA35
Specificity
The antibody IVA35 reacts with an extracellular epitope of CD18 antigen (bovine), a 90-95 kDa type I transmembrane protein (integrin) expressed on all leukocytes.
Purity/Purification
Purified by Protein-A Affinity Chromatography
Form/Format
Phosphate buffered saline (PBS) solution with 15 mM sodium azide
Concentration
1 mg/ml (varies by lot)
Sequence Length
769
Applicable Applications for anti-CD18 antibody
Flow Cytometry (FC/FACS), Immunoprecipitation (IP), Immunocytochemistry (ICC)
Immunogen
Bovine leukocytes.
Preparation and Storage
Store at 2-8 degree C.
Immunocytochemistry (ICC)
(Immunocytochemistry staining of acetone/methanol fixed bovine peripheral blood (lymphocyte-enriched suspension) using anti-bovine CD18 (IVA35); secondary antibody conjugated to FITC (green), DNA stained with DAPI (blue).)
Immunocytochemistry (ICC)
(Immunocytochemistry staining of acetone/methanol fixed bovine peripheral blood (lymphocyte-enriched suspension) using anti-bovine CD18 (IVA35); secondary antibody conjugated to FITC (green), DNA stained with DAPI (blue).)
Related Product Information for anti-CD18 antibody
CD18, integrin beta2 subunit, forms heterodimers with four types of CD11 molecule to constitute leukocyte (beta2) integrins: alphaLbeta2 (CD11a/CD18, LFA-1), alphaMbeta2 (CD11b/CD18, Mac-1, CR3), alphaXbeta2 (CD11c/CD18) and alphaDbeta2 (CD11d/CD18). In most cases, the response mediated by the integrin is a composite of the functions of its individual subunits. These integrins are essential for proper leukocyte migration, mediating intercellular contacts. Absence of CD18 leads to leukocyte adhesion deficiency-1; severe reduction of CD18 expression leads to the development of a psoriasiform skin disease. CD18 is also a target of Mannheimia (Pasteurella) haemolytica leukotoxin and is sufficient to mediate leukotoxin-mediated cytolysis.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
NCBI Official Full Name
Integrin beta-2
NCBI Official Synonym Full Names
integrin subunit beta 2
NCBI Official Symbol
ITGB2
NCBI Official Synonym Symbols
CD18
NCBI Protein Information
integrin beta-2
UniProt Protein Name
Integrin beta-2
Protein Family
UniProt Gene Name
ITGB2
UniProt Synonym Gene Names
CD18
Uniprot Description
Integrin alpha-L/beta-2 is a receptor for ICAM1, ICAM2, ICAM3 and ICAM4. Integrins alpha-M/beta-2 and alpha-X/beta-2 are receptors for the iC3b fragment of the third complement component and for fibrinogen. Integrin alpha-X/beta-2 recognizes the sequence G-P-R in fibrinogen alpha-chain. Integrin alpha-M/beta-2 recognizes P1 and P2 peptides of fibrinogen gamma chain. Integrin alpha-M/beta-2 is also a receptor for factor X. Integrin alpha-D/beta-2 is a receptor for ICAM3 and VCAM1. Contributes to natural killer cell cytotoxicity. Involved in leukocyte adhesion and transmigration of leukocytes including T-cells and neutrophils. Triggers neutrophil transmigration during lung injury through PTK2B/PYK2-mediated activation. Integrin alpha-L/beta-2 in association with ICAM3, contributes to apoptotic neutrophil phagocytosis by macrophages.
Research Articles on CD18
Similar Products
Product Notes
The CD18 itgb2 (Catalog #AAA1800812) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Anti-Bov CD18 Purified reacts with Cow and may cross-react with other species as described in the data sheet. AAA Biotech's CD18 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), Immunoprecipitation (IP), Immunocytochemistry (ICC). Researchers should empirically determine the suitability of the CD18 itgb2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD18, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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