Mouse anti-Human CD18 Antibody | anti-CD18 antibody

Anti-Hu CD18 Purified

Cat. Num. AAA1801219

• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, Immunoprecipitation, Western Blot, Immunohistochemistry
• Purity: Purified by Protein-A Affinity Chromatography
• Synonyms: CD18; Anti-Hu CD18 Purified; integrin subunit beta 2; Integrin beta2; Complement receptor C3 subunit beta; ITGB2; anti-CD18 antibody

• Host: Mouse
• Reactivity: Human
• Isotype: IgG1
• Clone Number: MEM-48
• Specificity: The antibody MEM-48 recognizes an extracellular epitope involving residues 534-546 in cysteine-rich repeat 3 of the CD18 antigen (integrin beta2 subunit; beta2 integrin). CD18 is a 90-95 kDa type I transmembrane protein expressed on all leukocytes.
• Purity/Purification: Purified by Protein-A Affinity Chromatography
• Form/Format: Phosphate buffered saline (PBS) solution with 15 mM sodium azide
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 769

• Applicable Applications for anti-CD18 antibody: Flow Cytometry (FC/FACS), Immunoprecipitation (IP), Western Blot (WB), Immunohistochemistry (IHC) Frozen/Paraffin
• Application Notes: FC/FACS: QC tested
• Immunogen: Leukocytes of a patient suffering from a LGL-type leukemia.
• Negative Species: Dog
• Preparation and Storage: Store at 2-8 degree C.

Immunohistochemistry (IHC)-Paraffin

(Immunohistochemistry staining of human spleen (paraffin sections) using anti-CD18 (MEM-48).Commercially tested by LifeSpan BioSciences.)

Related Product Information for anti-CD18 antibody

CD18, integrin beta2 subunit, forms heterodimers with four types of CD11 molecule to constitute leukocyte (beta2) integrins: alphaLbeta2 (CD11a/CD18, LFA-1), alphaMbeta2 (CD11b/CD18, Mac-1, CR3), alphaXbeta2 (CD11c/CD18) and alphaDbeta2 (CD11d/CD18). In most cases, the response mediated by the integrin is a composite of the functions of its individual subunits. These integrins are essential for proper leukocyte migration, mediating intercellular contacts. Absence of CD18 leads to leukocyte adhesion deficiency-1; severe reduction of CD18 expression leads to the development of a psoriasiform skin disease. CD18 is also a target of Mannheimia (Pasteurella) haemolytica leukotoxin and is sufficient to mediate leukotoxin-mediated cytolysis.

NCBI and Uniprot Product Information

• NCBI GI #: 89191865
• NCBI Accession #: NP_000202.2
• NCBI GenBank Nucleotide #: NP_000202.2
• UniProt Accession #: P05107
• NCBI Official Full Name: integrin beta-2
• UniProt Protein Name: Integrin beta-2
• Protein Family: CD180 antigen
• UniProt Gene Name: ITGB2
• UniProt Synonym Gene Names: CD18; MFI7
• UniProt Entry Name: ITB2_HUMAN

Uniprot Description

ITGB2: the integrin beta 2 subunit. Can combine with multiple partners resulting in different integrins. For example, beta 2 combines with the alpha L chain to form the integrin LFA-1, and combines with the alpha M chain to form the integrin Mac-1. Participates in cell adhesion as well as cell-surface mediated signaling. Defects are the cause of leukocyte adhesion deficiency type I (LAD1).

Protein type: Receptor, misc.; Membrane protein, integral; Cell adhesion; Motility/polarity/chemotaxis; Cell surface

Chromosomal Location of Human Ortholog: 21q22.3

Cellular Component: cell surface; membrane; plasma membrane; integrin complex; receptor complex; vesicle; lipid raft

Molecular Function: protein binding; protein heterodimerization activity; metal ion binding; protein complex binding; cell adhesion molecule binding; ICAM-3 receptor activity; glycoprotein binding; protein kinase binding

Biological Process: extracellular matrix organization and biogenesis; positive regulation of nitric oxide biosynthetic process; apoptosis; cell-matrix adhesion; natural killer cell activation; receptor clustering; activation of NF-kappaB transcription factor; regulation of cell shape; leukocyte adhesion; cellular extravasation; cell-cell signaling; inflammatory response; cell adhesion; regulation of peptidyl-tyrosine phosphorylation; toll-like receptor 4 signaling pathway; aging; integrin-mediated signaling pathway; neutrophil chemotaxis; regulation of immune response; activated T cell proliferation; leukocyte migration during inflammatory response; heterotypic cell-cell adhesion; positive regulation of angiogenesis; toll-like receptor signaling pathway; endothelial cell migration; innate immune response; blood coagulation; leukocyte migration

Disease: Leukocyte Adhesion Deficiency, Type I