Rat anti-Mouse CD169 Monoclonal Antibody | anti-CD169 antibody

CD169 (Sialoadhesin, Siglec-1) (AP)

Cat. Num. AAA6247506

• Gene Names: Siglec1; Sn; Cd169; Siglec-1
• Reactivity: Mouse
• Applications: Immunofluorescence, Immunohistochemistry
• Purity: Purified by Protein G Affinity Chromatography
• Synonyms: CD169; Monoclonal Antibody; CD169 (Sialoadhesin; Siglec-1) (AP); anti-CD169 antibody

• Host: Rat
• Reactivity: Mouse
• Clonality: Monoclonal
• Isotype: IgG2a
• Clone Number: MOMA-1
• Specificity: Recognizes mouse CD169.
• Purity/Purification: Purified by Protein G Affinity Chromatography
• Form/Format: Supplied as a liquid in PBS, pH 7.2. Labeled with Alkaline Phosphatase.

• Applicable Applications for anti-CD169 antibody: Immunofluorescence (IF), Immunohistochemistry (IHC)
• Immunogen: Stromal (reticular) elements from spleen.
• Conjugate: AP
• Preparation and Storage: Store at 4 degree C. Do Not Freeze.

Immunofluorescence (IF)

(Immunofluorescence staining of mouse spleen with Rat anti Mouse CD169)

Product Categories/Family for anti-CD169 antibody

Antibodies; CD Markers

References

1. Kraal, G. and Janse, M. (1986) Marginal metallophilic cells of the mouse spleen identified by a monoclonal antibody. Immunology. 58: 665-9. 2. Oetke, C. et al. (2006) The antigen recognized by MOMA-I is sialoadhesin. Immunol Lett. 106: 96-98. 3. Tumanov, A.V. et al. (2010) Cellular source and molecular form of TNF specify its distinct functions in organization of secondary lymphoid organs. Blood. Jul 15. 4. Karlsson, M.C. et al. (2003) Macrophages control the retention and trafficking of B lymphocytes in the splenic marginal zone. J Exp Med. 198: 333-40. 5. Kanayama, N. et al. (2005) Analysis of marginal zone B cell development in the mouse with limited B cell diversity: role of the antigen receptor signals in the recruitment of B cells to the marginal zone. J. Immunol. 174:1438-1445. 6. Ho:pken, U. E. et al. (2004) Distinct and overlapping roles of CXCR5 and CCR7 in B-1 cell homing and early immunity against bacterial pathogens. J. Leukoc. Biol. 76: 709-718. 7. Ferguson, A. R. et al (2004) Marginal zone B cells transport and deposit IgM-containing immune complexes onto follicular dendritic cells. Int. Immunol. 16: 1411-1422. 8. Girkontaite, J. et al. (2004) The sphingosine-1-phosphate (S1P) lysophospholipid receptor S1P3 regulates MAdCAM-1+ endothelial cells in splenic marginal sinus organization. J. Exp. Med. 200 : 1491-1501. 9. Acevedo-Suarez, C. A. et al. (2005) Uncoupling of anergy from developmental arrest in anti-insulin B cells supports the development of autoimmune diabetes. J. Immunol. 174:827-833. 10. Birjandi, S.Z. et al. (2011) Alterations in marginal zone macrophages and marginal zone B cells in old mice. J Immunol. 186: 3441-51. 11. Bhattacharyya, S. et al. (2011) NFATc1 affects mouse splenic B cell function by controlling the calcineurin-NFAT signaling network. J Exp Med. Apr 4. 12. Jang, I.K. et al. (2011) Growth-factor receptor-bound protein-2 (Grb2) signaling in B cells controls lymphoid follicle organization and germinal center reaction. Proc Natl Acad Sci U S A. 108: 7926-31. 13. Rehm, A. et al. (2011) Cooperative function of CCR7 and lymphotoxin in the formation of a lymphoma-permissive niche within murine secondary lymphoid organs. Blood. May 17. 14. Mattsson, J. et al. (2011) Complement activation and complement receptors on follicular dendritic cells are critical for the function of a targeted adjuvant. J Immunol. 187: 3641-52. 15. Whipple, E. C. et al. (2004) Analyses of the In Vivo Trafficking of Stoichiometric Doses of an Anti-Complement Receptor 1/2 Monoclonal Antibody Infused Intravenously in Mice. J. Immunol.173: 2297-2306. 16. Zhang, Z. et al. (2012) Notch-RBP-J-Independent Marginal Zone B Cell Development in IgH Transgenic Mice with V(H) Derived from a Natural Polyreactive Antibody. PLoS One. 7: e38894.

NCBI and Uniprot Product Information

• NCBI GI #: 347595712
• NCBI GeneID: 20612
• UniProt Accession #: Q62230; O55216; Q62228; Q62229; D3YVZ3; D3YVZ4
• Molecular Weight: 172,769 Da
• NCBI Official Full Name: Sialoadhesin
• NCBI Official Synonym Full Names: sialic acid binding Ig-like lectin 1, sialoadhesin
• NCBI Official Symbol: Siglec1
• NCBI Official Synonym Symbols: Sn; Cd169; Siglec-1
• NCBI Protein Information: sialoadhesin; SER; sheep erythrocyte receptor; sialic acid-binding Ig-like lectin 1
• UniProt Protein Name: Sialoadhesin
• UniProt Gene Name: Siglec1
• UniProt Synonym Gene Names: Sa; Sn; SER; Siglec-1
• UniProt Entry Name: SN_MOUSE

Uniprot Description

SIGLEC1: Acts as an endocytic receptor mediating clathrin dependent endocytosis. Macrophage-restricted adhesion molecule that mediates sialic-acid dependent binding to lymphocytes, including granulocytes, monocytes, natural killer cells, B-cells and CD8 T-cells. Preferentially binds to alpha-2,3-linked sialic acid. Binds to SPN/CD43 on T-cells. May play a role in hemopoiesis. Belongs to the immunoglobulin superfamily. SIGLEC (sialic acid binding Ig-like lectin) family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Cellular Component: membrane; extracellular region; integral to membrane; plasma membrane

Molecular Function: protein binding; carbohydrate binding

Biological Process: endocytosis; cell adhesion