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Western Blot (WB)
(PLCL2 Antibody (C-term) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the PLCL2 antibody detected the PLCL2 protein (arrow).)
Rabbit anti-Human PLCL2 Polyclonal Antibody | anti-PLCL2 antibody
PLCL2, CT (PLCL2, KIAA1092, PLCE2, Inactive phospholipase C-like protein 2, Phospholipase C-epsilon-2) (FITC)
Gene Names
PLCL2; PLCE2
Reactivity
Human
Applications
Flow Cytometry, Functional Assay, Immunofluorescence, Immunohistochemistry, Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
PLCL2; Polyclonal Antibody; CT (PLCL2; KIAA1092; PLCE2; Inactive phospholipase C-like protein 2; Phospholipase C-epsilon-2) (FITC); Phospholipase C-epsilon-2; anti-PLCL2 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).
Sequence Length
1001
Applicable Applications for anti-PLCL2 antibody
Flow Cytometry (FC/FACS), FLISA, Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blot (WB)
Application Notes
FC/FACS: 1:10-50
FLISA: 1:1,000
IF: 1:10-50
IHC: 1:10-50
WB: 1:100-500
Applications are based on unconjugated antibody.
FLISA: 1:1,000
IF: 1:10-50
IHC: 1:10-50
WB: 1:100-500
Applications are based on unconjugated antibody.
Immunogen
PLCL2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1100-1127 amino acids from the C-terminal region of human PLCL2.
Conjugate
FITC
Note
Preservative Free
Special Handling
Light sensitive
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(PLCL2 Antibody (C-term) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the PLCL2 antibody detected the PLCL2 protein (arrow).)
Western Blot (WB)
(PLCL2 Antibody (C-term) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the PLCL2 antibody detected the PLCL2 protein (arrow).)
Immunohistochemistry (IHC)
(PLCL2 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of PLCL2 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(PLCL2 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of PLCL2 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Immunofluorescence (IF)
(Confocal immunofluorescent analysis of PLCL2 Antibody (C-term) with NCI-H460 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).)
Immunofluorescence (IF)
(Confocal immunofluorescent analysis of PLCL2 Antibody (C-term) with NCI-H460 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).)
Flow Cytometry (FC/FACS)
(PLCL2 Antibody (C-term) flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.)
Flow Cytometry (FC/FACS)
(PLCL2 Antibody (C-term) flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.)
Related Product Information for anti-PLCL2 antibody
PLCL2 may play an role in the regulation of Ins(1,4,5)P3 around the endoplasmic reticulum (By similarity).
Product Categories/Family for anti-PLCL2 antibody
References
Raychaudhuri, S., et al. Nat. Genet. 41(12):1313-1318(2009). Colland, F., et al. Genome Res. 14(7):1324-1332(2004). Nakayama, M., et al. Genome Res. 12(11):1773-1784(2002). Otsuki, M., et al. Biochem. Biophys. Res. Commun. 266(1):97-103(1999).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
inactive phospholipase C-like protein 2 isoform 2
NCBI Official Synonym Full Names
phospholipase C like 2
NCBI Official Symbol
PLCL2
NCBI Official Synonym Symbols
PLCE2
NCBI Protein Information
inactive phospholipase C-like protein 2
UniProt Protein Name
Inactive phospholipase C-like protein 2
Protein Family
UniProt Gene Name
PLCL2
UniProt Synonym Gene Names
KIAA1092; PLCE2; PLC-L(2); PLC-L2; Phospholipase C-L2; PLC-epsilon-2
UniProt Entry Name
PLCL2_HUMAN
Research Articles on PLCL2
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Product Notes
The PLCL2 plcl2 (Catalog #AAA6334721) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PLCL2, CT (PLCL2, KIAA1092, PLCE2, Inactive phospholipase C-like protein 2, Phospholipase C-epsilon-2) (FITC) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PLCL2 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), FLISA, Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blot (WB). FC/FACS: 1:10-50 FLISA: 1:1,000 IF: 1:10-50 IHC: 1:10-50 WB: 1:100-500 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the PLCL2 plcl2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PLCL2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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