Western Blot (WB)
(Western Blot analysis of RNF146 expression in transfected 293T cell line by RNF146 polyclonal antibody. Lane1:RNF146 transfected lysate (39.38kD). Lane2:Non-transfected lysate.)
Mouse anti-Human RNF146 Polyclonal Antibody | anti-RNF146 antibody
RNF146 (E3 Ubiquitin-protein Ligase RNF146, Dactylidin, Iduna, RING Finger Protein 146)
Purified by Protein A affinity chromatography.
Purified by Protein A affinity chromatography.
Western Blot (WB)
(Western Blot analysis of RNF146 expression in transfected 293T cell line by RNF146 polyclonal antibody. Lane1:RNF146 transfected lysate (39.38kD). Lane2:Non-transfected lysate.)
Western Blot (WB)
(Western Blot analysis of RNF146 expression in transfected 293T cell line by RNF146 polyclonal antibody. Lane1:RNF146 transfected lysate (39.38kD). Lane2:Non-transfected lysate.)
NCBI and Uniprot Product Information
Uniprot Description
RNF146: E3 ubiquitin-protein ligase that specifically binds poly-ADP-ribosylated (PARsylated) proteins and mediates their ubiquitination and subsequent degradation. May regulate many important biological processes, such as cell survival and DNA damage response. Acts as an activator of the Wnt signaling pathway by mediating the ubiquitination of PARsylated AXIN1 and AXIN2, 2 key components of the beta-catenin destruction complex. Acts in cooperation with tankyrase proteins (TNKS and TNKS2), which mediate PARsylation of target proteins AXIN1, AXIN2, BLZF1, CASC3, TNKS and TNKS2. Recognizes and binds tankyrase-dependent PARsylated proteins via its WWE domain and mediates their ubiquitination, leading to their degradation. Different ubiquitin linkage types have been observed: TNKS2 undergoes ubiquination at 'Lys-48' and 'Lys-63', while AXIN1 is only ubiquitinated at 'Lys- 48'. May regulate TNKS and TNKS2 subcellular location, preventing aggregation at a centrosomal location. Neuroprotective protein. Protects the brain against N-methyl-D-aspartate (NMDA) receptor- mediated glutamate excitotoxicity and ischemia, by interfering with PAR-induced cell death, called parthanatos. Prevents nuclear translocation of AIFM1 in a PAR-binding dependent manner. Does not affect PARP1 activation. Protects against cell death induced by DNA damaging agents, such as N-methyl-N-nitro-N- nitrosoguanidine (MNNG) and rescues cells from G1 arrest. Promotes cell survival after gamma-irradiation. Facilitates DNA repair. Defects in RNF146 are a cause of susceptibility to breast cancer. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Ubiquitin conjugating system; EC 6.3.2.19; Ubiquitin ligase; EC 6.3.2.-
Chromosomal Location of Human Ortholog: 6q22.1-q22.33
Cellular Component: nucleus; cytosol
Molecular Function: protein binding; zinc ion binding; ubiquitin-protein ligase activity; ligase activity
Biological Process: protein autoubiquitination; Wnt receptor signaling pathway; protein ubiquitination during ubiquitin-dependent protein catabolic process
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Product Notes
The RNF146 rnf146 (Catalog #AAA6003694) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The RNF146 (E3 Ubiquitin-protein Ligase RNF146, Dactylidin, Iduna, RING Finger Protein 146) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's RNF146 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Suitable for use in Western Blot. Researchers should empirically determine the suitability of the RNF146 rnf146 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: MAGCGEIDHS INMLPTNRKA NESCSNTAPS LTVPECAICL QTCVHPVSLP CKHVFCYLCV KGASWLGKRC ALCRQEIPED FLDKPTLLSP EELKAASRGN GEYAWYYEGR NGWWQYDERT SRELEDAFSK GKKNTEMLIA GFLYVADLEN MVQYRRNEHG RRRKIKRDII DIPKKGVAGL RLDCDANTVN LARESSADGA DSVSAQSGAS VQPLVSSVRP LTSVDGQLTS PATPSPDAST SLEDSFAHLQ LSGDNTAERS HRGEGEEDHE SPSSGRVPAP DTSIEETESD ASSDSEDVSA VVAQHSLTQQ RLLVSNANQT VPDRSDRSGT DRSVAGGGTV SVSVRSRRPD GQCTVTEV. It is sometimes possible for the material contained within the vial of "RNF146, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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