Rabbit anti-Human FOLH1 Polyclonal Antibody | anti-FOLH1 antibody

FOLH1, NT (FOLH1, FOLH, NAALAD1, PSM, PSMA, Glutamate carboxypeptidase 2, Cell growth-inhibiting gene 27 protein, Folate hydrolase 1, Folylpoly-gamma-glutamate carboxypeptidase, Glutamate carboxypeptidase II, Membrane glutamate carboxypeptidase, N-acetyla

Cat. Num. AAA6299579

• Gene Names: FOLH1; PSM; FGCP; FOLH; GCP2; PSMA; mGCP; GCPII; NAALAD1; NAALAdase
• Reactivity: Human
• Applications: Immunofluorescence, ELISA, Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: FOLH1; Polyclonal Antibody; NT (FOLH1; FOLH; NAALAD1; PSM; PSMA; Glutamate carboxypeptidase 2; Cell growth-inhibiting gene 27 protein; Folate hydrolase 1; Folylpoly-gamma-glutamate carboxypeptidase; Glutamate carboxypeptidase II; Membrane glutamate carboxypeptidase; N-acetyla; N-acetylated-alpha-linked acidic dipeptidase I; Prostate-specific membrane antigen; Pteroylpoly-gamma-glutamate carboxypeptidase; anti-FOLH1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.
• Sequence Length: 735

• Applicable Applications for anti-FOLH1 antibody: Immunofluorescence (IF), ELISA (EIA), Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: IF: 1:10-50; ELISA: 1:1,000; IHC: 1:10-50; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: FOLH1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 160-190 amino acids from the N-terminal region of human FOLH1.
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(FOLH1 Antibody (N-term) western blot analysis in ZR-75-1 cell line lysates (35ug/lane).This demonstrates the FOLH1 antibody detected the FOLH1 protein (arrow).)

Immunohistochemistry (IHC)

(FOLH1 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human prostate carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of FOLH1 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Immunofluorescence (IF)

(Confocal immunofluorescent analysis of FOLH1 Antibody (N-term) with ZR-75-1 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).)

Related Product Information for anti-FOLH1 antibody

This gene encodes a type II transmembrane glycoprotein belonging to the M28 peptidase family. The protein acts as a glutamate carboxypeptidase on different alternative substrates, including the nutrient folate and the neuropeptide N-acetyl-l-aspartyl-l-glutamate and is expressed in a number of tissues such as prostate, central and peripheral nervous system and kidney. A mutation in this gene may be associated with impaired intestinal absorption of dietary folates, resulting in low blood folate levels and consequent hyperhomocysteinemia. Expression of this protein in the brain may be involved in a number of pathological conditions associated with glutamate excitotoxicity. In the prostate the protein is up-regulated in cancerous cells and is used as an effective test and prognostic indicator of prostate cancer. This gene likely arose from a duplication event of a nearby chromosomal region. Alternative splicing gives rise to multiple transcript variants encoding several different isoforms.

Product Categories/Family for anti-FOLH1 antibody

Antibodies; Enzymes; Carboxypeptidase

References

Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010). Giusti, B., et al. Thromb. Haemost. 104(2):231-242(2010). Jugessur, A., et al. PLoS ONE 5 (7), E11493 (2010): Mlcochova, P., et al. Prostate 69(5):471-479(2009). Davis, M.I., et al. Proc. Natl. Acad. Sci. U.S.A. 102(17):5981-5986(2005).

NCBI and Uniprot Product Information

• NCBI GI #: 301500668
• NCBI GeneID: 2346
• NCBI Accession #: NP_001180400.1; NP_001014986.1
• NCBI GenBank Nucleotide #: NP_001180400.1
• UniProt Accession #: Q04609
• NCBI Official Full Name: glutamate carboxypeptidase 2 isoform 3
• NCBI Official Synonym Full Names: folate hydrolase 1
• NCBI Official Symbol: FOLH1
• NCBI Official Synonym Symbols: PSM; FGCP; FOLH; GCP2; PSMA; mGCP; GCPII; NAALAD1; NAALAdase
• NCBI Protein Information: glutamate carboxypeptidase 2
• UniProt Protein Name: Glutamate carboxypeptidase 2
• Protein Family: Glutamate carboxypeptidase
• UniProt Gene Name: FOLH1
• UniProt Synonym Gene Names: FOLH; NAALAD1; PSM; PSMA; FGCP; GCPII; mGCP; NAALADase I; PSM; PSMA
• UniProt Entry Name: FOLH1_HUMAN

NCBI Description

This gene encodes a type II transmembrane glycoprotein belonging to the M28 peptidase family. The protein acts as a glutamate carboxypeptidase on different alternative substrates, including the nutrient folate and the neuropeptide N-acetyl-l-aspartyl-l-glutamate and is expressed in a number of tissues such as prostate, central and peripheral nervous system and kidney. A mutation in this gene may be associated with impaired intestinal absorption of dietary folates, resulting in low blood folate levels and consequent hyperhomocysteinemia. Expression of this protein in the brain may be involved in a number of pathological conditions associated with glutamate excitotoxicity. In the prostate the protein is up-regulated in cancerous cells and is used as an effective diagnostic and prognostic indicator of prostate cancer. This gene likely arose from a duplication event of a nearby chromosomal region. Alternative splicing gives rise to multiple transcript variants encoding several different isoforms. [provided by RefSeq, Jul 2010]

Uniprot Description

FOLH1: Has both folate hydrolase and N-acetylated-alpha-linked- acidic dipeptidase (NAALADase) activity. Has a preference for tri- alpha-glutamate peptides. In the intestine, required for the uptake of folate. In the brain, modulates excitatory neurotransmission through the hydrolysis of the neuropeptide, N- aceylaspartylglutamate (NAAG), thereby releasing glutamate. Isoform PSM-4 and isoform PSM-5 would appear to be physiologically irrelevant. Involved in prostate tumor progression. Belongs to the peptidase M28 family. M28B subfamily. 8 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; EC 3.4.17.21; Protease

Chromosomal Location of Human Ortholog: 11p11.2

Cellular Component: membrane; integral to plasma membrane; cytoplasm

Molecular Function: peptidase activity; dipeptidase activity; metallopeptidase activity; carboxypeptidase activity; metal ion binding

Biological Process: folic acid and derivative metabolic process; proteolysis