Typical Testing Data/Standard Curve
Mouse CD14 ELISA Kit | CD14 elisa kit
Mouse CD14 ELISA Kit
Reactivity
Mouse
Synonyms
CD14 ; Mouse CD14 ELISA Kit; CD14 elisa kit
Reactivity
Mouse
Samples
Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
Assay Type
Competitive
Detection Range
1.0-25ng/mL
Sensitivity
0.1ng/mL
Preparation and Storage
Store all reagents at 2-8 degree C.
Typical Testing Data/Standard Curve
Typical Testing Data/Standard Curve
Related Product Information for CD14 elisa kit
Intended Uses: This CD14 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse CD14. This ELISA kit for research use only!
Principle of the Assay||CD14 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-CD14 antibody and an CD14-HRP conjugate. The assay sample and buffer are incubated together with CD14-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CD14 concentration since CD14 from samples and CD14-HRP conjugate compete for the anti-CD14 antibody binding site. Since the number of sites is limited, as more sites are occupied by CD14 from the sample, fewer sites are left to bind CD14-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CD14 concentration in each sample is interpolated from this standard curve.
Principle of the Assay||CD14 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-CD14 antibody and an CD14-HRP conjugate. The assay sample and buffer are incubated together with CD14-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CD14 concentration since CD14 from samples and CD14-HRP conjugate compete for the anti-CD14 antibody binding site. Since the number of sites is limited, as more sites are occupied by CD14 from the sample, fewer sites are left to bind CD14-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CD14 concentration in each sample is interpolated from this standard curve.
Product Categories/Family for CD14 elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
39,667 Da
NCBI Official Full Name
CD14
NCBI Official Synonym Full Names
CD14 molecule
NCBI Official Symbol
CD14
NCBI Protein Information
monocyte differentiation antigen CD14; CD14 antigen; myeloid cell-specific leucine-rich glycoprotein
UniProt Protein Name
Monocyte differentiation antigen CD14
UniProt Gene Name
CD14
UniProt Entry Name
CD14_BOVIN
Uniprot Description
Coreceptor for bacterial lipopolysaccharide. In concert with LBP, binds to monomeric lipopolysaccharide and delivers it to the LY96/TLR4 complex, thereby mediating the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MyD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Acts as a coreceptor for TLR2:TLR6 heterodimer in response to diacylated lipopeptides and for TLR2:TLR1 heterodimer in response to triacylated lipopeptides, these clusters trigger signaling from the cell surface and subsequently are targeted to the Golgi in a lipid-raft dependent pathway. Binds electronegative LDL (LDL-) and mediates the cytokine release induced by LDL- ().
Research Articles on CD14
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Product Notes
The Mouse CD14 cd14 (Catalog #AAA729343) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA729343 ELISA Kit recognizes Mouse CD14 . It is sometimes possible for the material contained within the vial of "CD14 , ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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