Mouse anti-Human CD13 Monoclonal Antibody | anti-ANPEP antibody

Anti-CD13 Antibody, Mouse Monoclonal

Cat. Num. AAA8118883

• Reactivity: Human
• Applications: Western Blot, Immunoprecipitation
• Purity: Protein A
• Synonyms: CD13; Monoclonal Antibody; Anti-CD13 Antibody; Mouse Monoclonal; CD13 /ANPEP Antibody; Mouse MAb; Anti-APN Antibody; Anti-GP150 Antibody; Anti-LAP1 Antibody; Anti-P150 Antibody; Anti-PEPN Antibody; alanyl (membrane) aminopeptidase; anti-ANPEP antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: Mouse IgG1
• Clone Number: 19
• Specificity: Human CD13 /ANPEP
• Purity/Purification: Protein A
• Form/Format: Liquid. 0.2um filtered solution in PBS

• Applicable Applications for anti-ANPEP antibody: Western Blot (WB), Immunoprecipitation (IP)
• Application Notes: WB: 1:500-1:2000; IP: 5-10uL/mg of lysate
• Immunogen: Recombinant Human CD13 /ANPEP Protein
• Conjugation: Unconjugated
• Preparation: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human CD16b/FCGR3B (rh CD16b/FCGR3B; O75015.2; Met1-Ser200). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.; This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Western Blot (WB)

(Anti-ANPEP mouse monoclonal antibody at 1:500 dilution Lane A: Jurkat Whole Cell Lysate Lane B: HepG2 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Mouse IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:110 kDa Observed band size:140 kDa)

Related Product Information for anti-ANPEP antibody

Background: Aminopeptidase N (ANPEP or APN), also known as CD13, is a cell-surface metalloprotease located in the small-intestinal and renal microvillar membrane, as well as other plasma membranes. It belongs to the peptidase M1 family. CD13 plays a role in the final digestion of peptides generated from hydrolysis of proteins by gastric and pancreatic proteases and is involved in the metabolism of regulatory peptides by diverse cell types. CD13/APN is a potent regulator of angiogenesis which is essential for tumor invasion and metastasis, and its transcription in activated endothelial cells is induced by angiogenic growth factors via the RAS/MAPK pathway. In addition, this enzyme has been shown to participate in antigen processing and presentation, and accordingly, defects in this gene appear to be a cause of various types of leukemia or lymphoma and carcinomas.

References

Pasqualini R, et al. (2000) Aminopeptidase N is a receptor for tumor-homing peptides and a target for inhibiting angiogenesis.Cancer Res. 60(3): 722-7.Mabjeesh SJ, et al. (2005) Aminopeptidase N gene expression and abundance in caprine mammary gland is influenced by circulating plasma peptide. J Dairy Sci. 88(6): 2055-64.Dunkel B, et al. (2009) Neutrophil and platelet activation in equine recurrent airway obstruction is associated with increased neutrophil CD13 expression, but not platelet CD41/61 and CD62P or neutrophil-platelet aggregate formation. Vet Immunol Immunopathol. 131(1-2): 25-32.