ANPEP / APN / CD13 recombinant protein

Recombinant Mouse ANPEP / APN / CD13 Protein (His tag)

Cat. Num. AAA2547224

• Gene Names: Anpep; Apn; AP-M; AP-N; Cd13; P150
• Purity: > 97 % as determined by SDS-PAGE
• Synonyms: ANPEP / APN / CD13; Recombinant Mouse ANPEP / APN / CD13 Protein (His tag); Anpep; Apn; Cd13; Lap-1; Lap1; p150; ANPEP / APN / CD13 recombinant protein

• Host: Human Cells
• Purity/Purification: > 97 % as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH 7.4
• Sequence Length: 966

• Application Notes: The recombinant mouse ANPEP consists of 909 amino acids and has a calculated molecular mass of 104 kDa. As a result of glycosylation, the recombinant protein migrates as an approximately 120-130 kDa protein in SDS-PAGE under reducing conditions.
• Predicted N Terminal: Lys 66
• Endotoxin: < 1.0 EU per mug of the protein as determined by the LAL method
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -70 degree C

SDS-PAGE

Related Product Information for ANPEP / APN / CD13 recombinant protein

Background: Aminopeptidase N (ANPEP or APN), also known as CD13, is a cell-surface metalloprotease located in the small-intestinal and renal microvillar membrane, as well as other plasma membranes. It belongs to the peptidase M1 family. CD13 plays a role in the final digestion of peptides generated from hydrolysis of proteins by gastric and pancreatic proteases and is involved in the metabolism of regulatory peptides by diverse cell type s. CD13/APN is a potent regulator of angiogenesis which is essential for tumor invasion and metastasis, and its transcription in activated endothelial cells is induced by angiogenic growth factors via the RAS/MAPK pathway. In addition, this enzyme has been shown to participate in antigen processing and presentation, and accordingly, defects in this gene appear to be a cause of various type s of leukemia or lymphoma and carcinomas.

Description: A DNA sequence encoding the metalloprotease domain (Lys 66-Ser 966) of mouse ANPEP (NP_032512.2) was expressed with a C-terminal polyhistidine tag.

NCBI and Uniprot Product Information

• NCBI GI #: 225637487
• NCBI GeneID: 16790
• NCBI Accession #: NP_032512.2
• NCBI GenBank Nucleotide #: NM_008486.2
• UniProt Accession #: P97449; Q91YH8; Q99K63
• Molecular Weight: 109,651 Da
• NCBI Official Full Name: aminopeptidase N
• NCBI Official Synonym Full Names: alanyl (membrane) aminopeptidase
• NCBI Official Symbol: Anpep
• NCBI Official Synonym Symbols: Apn; AP-M; AP-N; Cd13; P150
• NCBI Protein Information: aminopeptidase N
• UniProt Protein Name: Aminopeptidase N
• UniProt Gene Name: Anpep
• UniProt Synonym Gene Names: Lap-1; Lap1; AP-N; mAPN; AP-M

Uniprot Description

CD13: Broad specificity aminopeptidase. Plays a role in the final digestion of peptides generated from hydrolysis of proteins by gastric and pancreatic proteases. May play a critical role in the pathogenesis of cholesterol gallstone disease. May be involved in the metabolism of regulatory peptides of diverse cell types including small intestinal and tubular epithelial cells, macrophages, granulocytes and synaptic membranes from the CNS. Found to cleave antigen peptides bound to major histocompatibility complex class II molecules of presenting cells and to degrade neurotransmitters at synaptic junctions. Is also implicated as a regulator of IL-8 bioavailability in the endometrium, and therefore may contribute to the regulation of angiogenesis. Is used as a marker for acute myeloid leukemia and plays a role in tumor invasion. In case of human coronavirus 229E (HCoV-229E) infection, serves as receptor for HCoV-229E spike glycoprotein. Mediates as well human cytomegalovirus (HCMV) infection. Belongs to the peptidase M1 family.

Protein type: EC 3.4.11.2; Membrane protein, integral; Other Amino Acids Metabolism - glutathione; Protease; Receptor, misc.

Chromosomal Location of Human Ortholog: 7|7 D2

Cellular Component: cytoplasm; ER-Golgi intermediate compartment; external side of plasma membrane; extracellular space; lysosomal membrane; vesicle membrane

Molecular Function: aminopeptidase activity; peptide binding; zinc ion binding

Biological Process: aromatic compound metabolic process; peptide catabolic process; proteolysis