Rabbit anti-Human, Mouse NFAT1 Polyclonal Antibody | anti-NFAT1 antibody

NFAT1 (Phospho-Ser326) Antibody

Cat. Num. AAA9416493

• Gene Names: NFATC2; NFAT1; NFATP
• Reactivity: Human, Mouse
• Applications: Western Blot
• Purity: Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
• Synonyms: NFAT1; Polyclonal Antibody; NFAT1 (Phospho-Ser326) Antibody; NFATC2; NF-ATc2; NF-ATp; NFAT pre-existing subunit; NFATP; anti-NFAT1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Specificity: NFAT1 (Phospho-Ser326) Antibody detects endogenous levels of NFAT1 only when phosphorylated at Ser326
• Purity/Purification: Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
• Form/Format: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 901

• Applicable Applications for anti-NFAT1 antibody: Western Blot (WB)
• Application Notes: Western Blot: 1:1000
• Immunogen: A synthesized peptide derived from human NFAT1 (Phospho-Ser326)
• Immunogen Type: Peptide
• Modification: Phospho-Ser326
• Preparation and Storage: Store at -20 degree C

Western Blot (WB)

(Western blot analysis NFAT1 (Phospho-Ser326) using ATP1 Rat braintissue lysates)

Product Categories/Family for anti-NFAT1 antibody

Phospho-specific Ab

NCBI and Uniprot Product Information

• NCBI GI #: 209862843
• NCBI GeneID: 4773
• NCBI Accession #: NP_001129493.1
• NCBI GenBank Nucleotide #: NM_001136021.2
• UniProt Accession #: Q13469; Q13468; Q5TFW7; Q5TFW8; Q9NPX6; Q9NQH3; Q9UJR2; B5B2N8; B5B2N9; B5B2P0; B5B2P2; B5B2P3
• Molecular Weight: 76,700 Da
• NCBI Official Full Name: nuclear factor of activated T-cells, cytoplasmic 2 isoform D
• NCBI Official Synonym Full Names: nuclear factor of activated T-cells 2
• NCBI Official Symbol: NFATC2
• NCBI Official Synonym Symbols: NFAT1; NFATP
• NCBI Protein Information: nuclear factor of activated T-cells, cytoplasmic 2
• UniProt Protein Name: Nuclear factor of activated T-cells, cytoplasmic 2
• UniProt Gene Name: NFATC2
• UniProt Synonym Gene Names: NFAT1; NFATP; NF-ATc2; NFATc2; NF-ATp
• UniProt Entry Name: NFAC2_HUMAN

NCBI Description

This gene is a member of the nuclear factor of activated T cells (NFAT) family. The product of this gene is a DNA-binding protein with a REL-homology region (RHR) and an NFAT-homology region (NHR). This protein is present in the cytosol and only translocates to the nucleus upon T cell receptor (TCR) stimulation, where it becomes a member of the nuclear factors of activated T cells transcription complex. This complex plays a central role in inducing gene transcription during the immune response. Alternate transcriptional splice variants encoding different isoforms have been characterized. [provided by RefSeq, Apr 2012]

Uniprot Description

NFAT1: a transcription factor that plays a role in the inducible expression of cytokine genes in T cells, especially in the induction of the IL-2, IL-3, IL-4, TNF-alpha and GM-CSF. It is present in a phosphorylated the cytosol of unstimulated T cells. After T-cell activation, it is dephosphorylated by calcineurin and translocates to the nucleus as a part of a transcription complex. Two splice variant isoforms have been identified.

Protein type: DNA-binding; Transcription factor

Chromosomal Location of Human Ortholog: 20q13.2

Cellular Component: actin cytoskeleton; cytoplasm; cytosol; nucleoplasm; nucleus; plasma membrane

Molecular Function: DNA binding; protein binding; transcription factor activity

Biological Process: B cell receptor signaling pathway; cell migration; positive regulation of B cell proliferation; positive regulation of transcription, DNA-dependent; regulation of transcription, DNA-dependent; response to DNA damage stimulus; response to drug