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Flow Cytometry (FC/FACS) (Separation of human CD109 positive cells (red-filled) from CD109 negative cells (black-dashed) in flow cytometry analysis (surface staining) of human PHA stimulated peripheral blood mononuclear cells stained using anti-human CD109 (W7C5) PE antibody (10 ul reagent per milion cells in 100 ul of cell suspension).)

Mouse anti-Human CD109 Monoclonal Antibody | anti-CD109 antibody

Anti-Hu CD109 PE

Gene Names
CD109; p180; r150; CPAMD7
Reactivity
Human
Applications
Flow Cytometry, Functional Assay
Purity
The purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography.
Synonyms
CD109; Monoclonal Antibody; Anti-Hu CD109 PE; CD109 molecule; CPAMD7; p180; r150; FLJ38569; anti-CD109 antibody
Ordering
For Research Use Only!
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1
Clone Number
W7C5
Specificity
The mouse monoclonal antibody W7C5 recognizes CD109, an approximately 165 kDa GPI-anchored extracellular protein expressed mainly on various hematopoietic cells, activated T lymphoblasts, activated platelets, and endothelial cells.
Purity/Purification
The purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography.
Form/Format
Stabilizing phosphate buffered saline (PBS) solution containing 15 mM sodium azide
Sequence Length
1368
Applicable Applications for anti-CD109 antibody
Flow Cytometry (FC/FACS)
Application Notes
FC/FACS: QC tested
Immunogen
WERI-RB-1 retinoblastoma cell line
Conjugation
PE
Preparation and Storage
Store in the dark at 2-8 degree C. Do not freeze. Avoid prolonged exposure to light.

Flow Cytometry (FC/FACS)

(Separation of human CD109 positive cells (red-filled) from CD109 negative cells (black-dashed) in flow cytometry analysis (surface staining) of human PHA stimulated peripheral blood mononuclear cells stained using anti-human CD109 (W7C5) PE antibody (10 ul reagent per milion cells in 100 ul of cell suspension).)

Flow Cytometry (FC/FACS) (Separation of human CD109 positive cells (red-filled) from CD109 negative cells (black-dashed) in flow cytometry analysis (surface staining) of human PHA stimulated peripheral blood mononuclear cells stained using anti-human CD109 (W7C5) PE antibody (10 ul reagent per milion cells in 100 ul of cell suspension).)
Related Product Information for anti-CD109 antibody
CD109, also known as the Gov platelet alloantigen, is a GPI-anchored glycoprotein which localizes to the surface of platelets, activated T-cells, and endothelial cells, as well as of various hematopoietic cells and T cell lines. The protein binds to and negatively regulates signaling by transforming growth factor beta (TGF-beta). Multiple transcript variants encoding different isoforms have been found for this gene. The Gov antigen system is involved in platelet transfusion reaction, posttransfusion purpura and in neonatal alloimmune thrombocytopenia.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
CD109 antigen isoform 3 preproprotein
NCBI Official Synonym Full Names
CD109 molecule
NCBI Official Symbol
CD109
NCBI Official Synonym Symbols
p180; r150; CPAMD7
NCBI Protein Information
CD109 antigen
UniProt Protein Name
CD109 antigen
Protein Family
UniProt Gene Name
CD109
UniProt Synonym Gene Names
CPAMD7
UniProt Entry Name
CD109_HUMAN

NCBI Description

This gene encodes a glycosyl phosphatidylinositol (GPI)-linked glycoprotein that localizes to the surface of platelets, activated T-cells, and endothelial cells. The protein binds to and negatively regulates signalling by transforming growth factor beta (TGF-beta). Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2014]

Uniprot Description

CD109: Modulates negatively TGFB1 signaling in keratinocytes. Belongs to the protease inhibitor I39 (alpha-2- macroglobulin) family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, GPI anchor

Chromosomal Location of Human Ortholog: 6q13

Cellular Component: extracellular space; cell surface; plasma membrane

Molecular Function: serine-type endopeptidase inhibitor activity; transforming growth factor beta binding

Biological Process: hair follicle development; negative regulation of protein amino acid phosphorylation; negative regulation of transforming growth factor beta receptor signaling pathway; regulation of keratinocyte differentiation

Research Articles on CD109

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Product Notes

The CD109 cd109 (Catalog #AAA1801710) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Anti-Hu CD109 PE reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CD109 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS). FC/FACS: QC tested. Researchers should empirically determine the suitability of the CD109 cd109 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD109, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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