Rabbit anti-Human, Mouse CD109 Polyclonal Antibody | anti-CD109 antibody

CD109, NT (CD109, CPAMD7, CD109 antigen, 150kD TGF-beta-1-binding protein, C3 and PZP-like alpha-2-macroglobulin domain-containing protein 7, Platelet-specific Gov antigen, p180, r150, CD109) (Biotin)

Cat. Num. AAA6282731

• Gene Names: CD109; p180; r150; CPAMD7
• Reactivity: Human, Mouse
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: CD109; Polyclonal Antibody; NT (CD109; CPAMD7; CD109 antigen; 150kD TGF-beta-1-binding protein; C3 and PZP-like alpha-2-macroglobulin domain-containing protein 7; Platelet-specific Gov antigen; p180; r150; CD109) (Biotin); anti-CD109 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-CD109 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: CD109 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 77-109 amino acids from the N-terminal region of human CD109.
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(CD109 Antibody (N-term) western blot analysis in mouse cerebellum tissue lysates (35ug/lane).This demonstrates the CD109 antibody detected the CD109 protein (arrow).)

Related Product Information for anti-CD109 antibody

CD109 is a member of the alpha2-macroglobulin/complement superfamily. The encoded GPI-linked glycoprotein is found on the cell surface of platelets, activated T-cells, and endothelial cells. The protein binds to and negatively regulates signaling of transforming growth factor beta (TGF-beta).

Product Categories/Family for anti-CD109 antibody

Antibodies; CD Markers

References

Nie, Y.M., et al. Transfus Med 20(6):376-382(2010). Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010): Hagiwara, S., et al. Oncogene 29(15):2181-2191(2010). Bhatti, F.A., et al. Transfus Med 20(2):78-87(2010). Raychaudhuri, S., et al. Nat. Genet. 41(12):1313-1318(2009).

NCBI and Uniprot Product Information

• NCBI GI #: 227430301
• NCBI GeneID: 135228
• NCBI Accession #: NP_001153059.1; NP_001153060.1; NP_598000.2
• NCBI GenBank Nucleotide #: NM_001159587.2
• UniProt Accession #: Q6YHK3; Q0P6K7; Q5SYA8; Q5XUM7; Q5XUM9; Q6MZI7; Q8N3A7; Q8N915; Q8TDJ2; A5YKK4; B2R948; B3KW25
• Molecular Weight: 159,696 Da
• NCBI Official Full Name: CD109 antigen isoform 2 preproprotein
• NCBI Official Synonym Full Names: CD109 molecule
• NCBI Official Symbol: CD109
• NCBI Official Synonym Symbols: p180; r150; CPAMD7
• NCBI Protein Information: CD109 antigen
• UniProt Protein Name: CD109 antigen
• Protein Family: CD109 antigen
• UniProt Gene Name: CD109
• UniProt Synonym Gene Names: CPAMD7
• UniProt Entry Name: CD109_HUMAN

NCBI Description

This gene encodes a glycosyl phosphatidylinositol (GPI)-linked glycoprotein that localizes to the surface of platelets, activated T-cells, and endothelial cells. The protein binds to and negatively regulates signalling by transforming growth factor beta (TGF-beta). Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2014]

Uniprot Description

CD109: Modulates negatively TGFB1 signaling in keratinocytes. Belongs to the protease inhibitor I39 (alpha-2- macroglobulin) family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, GPI anchor

Chromosomal Location of Human Ortholog: 6q13

Cellular Component: extracellular space; cell surface; plasma membrane

Molecular Function: serine-type endopeptidase inhibitor activity; transforming growth factor beta binding

Biological Process: hair follicle development; negative regulation of protein amino acid phosphorylation; negative regulation of transforming growth factor beta receptor signaling pathway; regulation of keratinocyte differentiation