Western Blot (WB)
(Western blot detection of Caspase-11 in C6,3T3,Hela cell lysates using Caspase-11 Rabbit mAb(1:1000 diluted). Predicted band size:43kDa. Observed band size: 43,38,25kDa.)
Rabbit anti-Mouse Caspase-11 Monoclonal Antibody | anti-Casp4 antibody
Caspase-11 Rabbit mAb
IP: 1:20
Western Blot (WB)
(Western blot detection of Caspase-11 in C6,3T3,Hela cell lysates using Caspase-11 Rabbit mAb(1:1000 diluted). Predicted band size:43kDa. Observed band size: 43,38,25kDa.)
Western Blot (WB)
(Western blot detection of Caspase-11 in C6,3T3,Hela cell lysates using Caspase-11 Rabbit mAb(1:1000 diluted). Predicted band size:43kDa. Observed band size: 43,38,25kDa.)
Proinflammatory caspase (PubMed:8702803, PubMed:9038361, PubMed:25119034). Essential effector of NLRP3 inflammasome-dependent CASP1 activation and IL1B and IL18 secretion in response to non-canonical activators, such as UVB radiation, cholera enterotoxin subunit B and cytosolic LPS, as well as infection with Gram-negative bacteria (PubMed:22002608). Independently of NLRP3 inflammasome and CASP1, promotes pyroptosis, through GSDMD cleavage and activation, and IL1A, IL18 and HMGB1 release in response to non-canonical inflammasome activators (PubMed:22002608, PubMed:26320999, PubMed:26375003). Plays a crucial role in the restriction of Salmonella typhimurium replication in colonic epithelial cells during infection. In later stages of the infection (>3 days post infection), LPS from cytosolic Salmonella triggers CASP4 activation, which ultimately results in the pyroptosis of the infected cells and their extrusion into the gut lumen, as well as in IL18 secretion. Pyroptosis limits bacterial replication, while cytokine secretion promotes the recruitment and activation of immune cells and triggers mucosal inflammation (PubMed:25121752). Involved in LPS-induced IL6 secretion; this activity may not require caspase enzymatic activity. Involved in cell death induced by endoplasmic reticulum stress. Activated by direct binding to LPS without the need of an upstream sensor (PubMed:25119034). Does not directly process IL1B (PubMed:8702803, PubMed:9038361). During non-canonical inflammasome activation, cuts CGAS and may play a role in the regulation of antiviral innate immune activation (PubMed:28314590).
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the cysteine proteases that plays important roles in apoptosis, cell migration and the inflammatory response. The encoded protein mediates production of pro-inflammatory cytokines by macrophages upon bacterial infection. Mice lacking the encoded protein are resistant to endotoxic shock induced by lipopolysaccharide. A 5-bp deletion encompassing a splice acceptor junction resulting in alternate splicing and a shorter non-functional isoform in certain mouse strains has been described. Although its official nomenclature is "caspase 4, apoptosis-related cysteine peptidase", this gene and its encoded protein have historically been called caspase 11. This gene is present in a cluster of three caspase genes on chromosome 9. [provided by RefSeq, Apr 2015]
Uniprot Description
CASP4: Involved in the activation cascade of caspases responsible for apoptosis execution. Cleaves caspase-1. Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a small and a large subunit. Widely expressed, with highest levels in spleen and lung. Moderate expression in heart and liver, low expression in skeletal muscle, kidney and testis. Not found in the brain. Belongs to the peptidase C14A family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Protease; EC 3.4.22.64; Apoptosis
Cellular Component: cell soma; endoplasmic reticulum; membrane; mitochondrion; neuron projection; protein complex
Molecular Function: cysteine-type endopeptidase activity; cysteine-type peptidase activity; hydrolase activity; peptidase activity
Biological Process: actin filament organization; apoptosis; germ cell programmed cell death; positive regulation of interleukin-1 beta secretion; proteolysis; regulation of apoptosis; regulation of inflammatory response
Research Articles on Casp4
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Product Notes
The Casp4 casp4 (Catalog #AAA4752628) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Caspase-11 Rabbit mAb reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Caspase-11 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunoprecipitation (IP). WB: 1:1000 IP: 1:20. Researchers should empirically determine the suitability of the Casp4 casp4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Caspase-11, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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