Western Blot (WB)
(Western Blot detection against Immunogen (37.66kD).)
Mouse anti-Human CH25H Monoclonal Antibody | anti-CH25H antibody
CH25H (Cholesterol 25-hydroxylase, Cholesterol 25-monooxygenase, h25OH) (AP)
Gene Names
CH25H; C25H
Reactivity
Human
Applications
ELISA, Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
CH25H; Monoclonal Antibody; CH25H (Cholesterol 25-hydroxylase; Cholesterol 25-monooxygenase; h25OH) (AP); anti-CH25H antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG2b,k
Clone Number
1G8
Specificity
Recognizes human CH25H.
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).
Applicable Applications for anti-CH25H antibody
ELISA (EIA), Western Blot (WB)
Application Notes
Applications are based on unconjugated antibody.
Immunogen
Partial recombinant protein corresponding to aa142-247 from CH25H with GST tag. MW of the GST tag alone is 26kD.
Immunogen Sequence
WHLLHHKVPWLYRTFHKVHHQNSSSFALATQYMSVWELFSLGFFDMMNVTLLGCHPLTTLTFHVVNIWLSVEDHSGYNFPWSTHRLVPFGWYGGVVHHDLHHSHFN
Conjugate
AP
Preparation and Storage
Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Western Blot (WB)
(Western Blot detection against Immunogen (37.66kD).)
Western Blot (WB)
(Western Blot detection against Immunogen (37.66kD).)
Related Product Information for anti-CH25H antibody
Catalyzes the formation of 25-hydroxycholesterol from cholesterol, leading to repress cholesterol biosynthetic enzymes. May play an important role in regulating lipid metabolism by synthesizing a corepressor that blocks sterol regulatory element binding protein (SREBP) processing. In testis, production of 25-hydroxycholesterol by macrophages may play a role in Leydig cell differentiation.
Product Categories/Family for anti-CH25H antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
31,745 Da
NCBI Official Full Name
Homo sapiens cholesterol 25-hydroxylase, mRNA
NCBI Official Synonym Full Names
cholesterol 25-hydroxylase
NCBI Official Symbol
CH25H
NCBI Official Synonym Symbols
C25H
NCBI Protein Information
cholesterol 25-hydroxylase
Protein Family
NCBI Description
This is an intronless gene that is involved in cholesterol and lipid metabolism. The encoded protein is a membrane protein and contains clusters of histidine residues essential for catalytic activity. Unlike most other sterol hydroxylases, this enzyme is a member of a small family of enzymes that utilize diiron cofactors to catalyze the hydroxylation of hydrophobic substrates. [provided by RefSeq, Jul 2008]
Research Articles on CH25H
Similar Products
Product Notes
The CH25H (Catalog #AAA6130566) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The CH25H (Cholesterol 25-hydroxylase, Cholesterol 25-monooxygenase, h25OH) (AP) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CH25H can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB). Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the CH25H for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CH25H, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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