Rabbit anti-Human, Mouse CASP4 Polyclonal Antibody | anti-CASP4 antibody

CASP4 Antibody

Cat. Num. AAA7047805

• Gene Names: CASP4; TX; Mih1; ICH-2; Mih1/TX; ICEREL-II; ICE(rel)II
• Reactivity: Human, Mouse
• Applications: ELISA, Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: >95%, Protein G purified
• Synonyms: CASP4; Polyclonal Antibody; CASP4 Antibody; Caspase-4; ICE and Ced-3 homolog 2; ICH2; anti-CASP4 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: >95%, Protein G purified
• Form/Format: Liquid
• Sequence Length: 377

• Applicable Applications for anti-CASP4 antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: WB: 1: 500-1: 5000; IHC: 1: 20-1: 200; IF: 1: 50-1: 200
• Immunogen: Recombinant Human Caspase-4 protein (82-226AA)
• Preservative: 0.03% Proclin 300
• Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
• Conjugation: Non-conjugated
• Santa Cruz Alternative: Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-1229 / sc-22174 / sc-28229
• Preparation and Storage: Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C or -80 degree C. Avoid repeated freeze.

Western Blot (WB)

(Western BlotPositive WB detected in:Mouse kidney tissue,Mouse brain tissue,Mouse skeletal muscle tissueAll lanes: CASP4 antibody at 3.5ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 44,37,14,31,19 kDaObserved band size: 44 kDa)

Immunohistochemistry (IHC)

(IHC image of MBS7047805 diluted at 1:700 and staining in paraffin-embedded human pancreatic cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.)

Immunohistochemistry (IHC)

(Immunofluorescent analysis of HepG2 cells using MBS7047805 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))

Related Product Information for anti-CASP4 antibody

Inflammatory caspase (PubMed: 7797510, PubMed: 23516580, PubMed: 25119034). Essential effector of NLRP3 inflammasome-dependent CASP1 activation and IL1B and IL18 secretion in response to non-canonical activators, such as UVB radiation, cholera enterotoxin subunit B and cytosolic LPS (PubMed: 22246630, PubMed: 26174085, PubMed: 26173988, PubMed: 26508369, PubMed: 25964352). Independently of NLRP3 inflammasome and CASP1, promotes pyroptosis, through GSDMD cleavage and activation, and IL1A, IL18 and HMGB1 release in response to non-canonical inflammasome activators (PubMed: 24879791, PubMed: 25964352). Plays a crucial role in the restriction of Salmonella typhimurium replication in colonic epithelial cells during infection (PubMed: 25121752). In later stages of the infection, LPS from cytosolic Salmonella triggers CASP4 activation, which ultimately results in pyroptosis of infected cells and their extrusion into the gut lumen, as well as in IL18 secretion. Pyroptosis limits bacterial replication, while cytokine secretion promotes the recruitment and activation of immune cells and triggers mucosal inflammation. Involved in LPS-induced IL6 secretion; this activity may not require caspase enzymatic activity (PubMed: 26508369). Involved in cell death induced by endoplasmic reticulum stress and by treatment with cytotoxic APP peptides found Alzheimer's patient brains (PubMed: 15123740, PubMed: 22246630, PubMed: 23661706). Activated by direct binding to LPS without the need of an upstream sensor (PubMed: 25119034). Does not directly process IL1B (PubMed: 7743998, PubMed: 7797592, PubMed: 7797510).

NCBI and Uniprot Product Information

• NCBI GI #: 4502577
• NCBI GeneID: 837
• NCBI Accession #: NP_001216.1
• NCBI GenBank Nucleotide #: NM_001225.3
• UniProt Accession #: P49662; O95601; Q7KYX7; Q9UG96; A2NHL8; A2NHL9; A2NHM0; B3KPZ9; B4DJH5; B4E2D2
• Molecular Weight: 18,111 Da
• NCBI Official Full Name: caspase-4 isoform alpha
• NCBI Official Synonym Full Names: caspase 4
• NCBI Official Symbol: CASP4
• NCBI Official Synonym Symbols: TX; Mih1; ICH-2; Mih1/TX; ICEREL-II; ICE(rel)II
• NCBI Protein Information: caspase-4
• UniProt Protein Name: Caspase-4
• Protein Family: Caspase
• UniProt Gene Name: CASP4
• UniProt Synonym Gene Names: ICH2; ICH-2
• UniProt Entry Name: CASP4_HUMAN

NCBI Description

This gene encodes a protein that is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes composed of a prodomain and a large and small protease subunit. Activation of caspases requires proteolytic processing at conserved internal aspartic residues to generate a heterodimeric enzyme consisting of the large and small subunits. This caspase is able to cleave and activate its own precursor protein, as well as caspase 1 precursor. When overexpressed, this gene induces cell apoptosis. Alternative splicing results in transcript variants encoding distinct isoforms. [provided by RefSeq, Jul 2008]

Uniprot Description

CASP4: Involved in the activation cascade of caspases responsible for apoptosis execution. Cleaves caspase-1. Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a small and a large subunit. Widely expressed, with highest levels in spleen and lung. Moderate expression in heart and liver, low expression in skeletal muscle, kidney and testis. Not found in the brain. Belongs to the peptidase C14A family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Apoptosis; EC 3.4.22.64; Protease

Chromosomal Location of Human Ortholog: 11q22.2-q22.3

Cellular Component: endoplasmic reticulum; mitochondrion

Biological Process: apoptosis; regulation of inflammatory response