Rat Fibrinogen Beta (FGb) ELISA Kit | FGb elisa kit

Rat Fibrinogen Beta (FGb) ELISA Kit

Cat. Num. AAA2024595

• Gene Names: FGB; HEL-S-78p
• Reactivity: Rat
• Synonyms: Fibrinogen Beta (FGb); Rat Fibrinogen Beta (FGb) ELISA Kit; FG-B; Fibrinopeptide B; FGb elisa kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of Fibrinogen Beta (FGb).; No significant cross-reactivity or interference between Fibrinogen Beta (FGb) and analogues was observed.
• Sequence Length: 491

• Assay Type: Double-antibody Sandwich
• Samples: Plasma
• Detection Range: 1.56-100ng/mL
• Sensitivity: < 0.73ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Fibrinogen Beta (FGb) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Fibrinogen Beta (FGb) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for FGb elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Fibrinogen Beta (FGb). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Fibrinogen Beta (FGb). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Fibrinogen Beta (FGb), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Fibrinogen Beta (FGb) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for FGb elisa kit

Signal transduction; Infection immunity; Hematology

NCBI and Uniprot Product Information

• NCBI GI #: 70906435
• NCBI GeneID: 2244
• NCBI Accession #: NP_005132.2
• NCBI GenBank Nucleotide #: NM_005141.4
• UniProt Accession #: P02675; Q32Q65; Q3KPF2; A0JLR9; B2R7G3
• Molecular Weight: 55,928 Da
• NCBI Official Full Name: fibrinogen beta chain isoform 1 preproprotein
• NCBI Official Synonym Full Names: fibrinogen beta chain
• NCBI Official Symbol: FGB
• NCBI Official Synonym Symbols: HEL-S-78p
• NCBI Protein Information: fibrinogen beta chain
• UniProt Protein Name: Fibrinogen beta chain
• Protein Family: Fibrinogen
• UniProt Gene Name: FGB
• UniProt Entry Name: FIBB_HUMAN

NCBI Description

The protein encoded by this gene is the beta component of fibrinogen, a blood-borne glycoprotein comprised of three pairs of nonidentical polypeptide chains. Following vascular injury, fibrinogen is cleaved by thrombin to form fibrin which is the most abundant component of blood clots. In addition, various cleavage products of fibrinogen and fibrin regulate cell adhesion and spreading, display vasoconstrictor and chemotactic activities, and are mitogens for several cell types. Mutations in this gene lead to several disorders, including afibrinogenemia, dysfibrinogenemia, hypodysfibrinogenemia and thrombotic tendency. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2014]

Uniprot Description

FGB: Fibrinogen has a double function: yielding monomers that polymerize into fibrin and acting as a cofactor in platelet aggregation. Defects in FGB are a cause of congenital afibrinogenemia (CAFBN). This rare autosomal recessive disorder is characterized by bleeding that varies from mild to severe and by complete absence or extremely low levels of plasma and platelet fibrinogen. Patients with congenital fibrinogen abnormalities can manifest different clinical pictures. Some cases are clinically silent, some show a tendency toward bleeding and some show a predisposition for thrombosis with or without bleeding.

Protein type: Cell surface; Adaptor/scaffold; Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 4q28

Cellular Component: extracellular space; cell surface; fibrinogen complex; extracellular region; plasma membrane; cell cortex; vesicle; external side of plasma membrane

Molecular Function: protein binding, bridging; protein binding; chaperone binding; cell adhesion molecule binding; structural molecule activity; receptor binding

Biological Process: protein polymerization; platelet activation; extracellular matrix organization and biogenesis; positive regulation of heterotypic cell-cell adhesion; platelet degranulation; cellular protein complex assembly; positive regulation of protein secretion; cell-matrix adhesion; positive regulation of vasoconstriction; innate immune response; signal transduction; blood coagulation; response to calcium ion; positive regulation of exocytosis

Disease: Afibrinogenemia, Congenital; Dysfibrinogenemia, Congenital