Mouse CaM Kinase IV Monoclonal Antibody | anti-CaM antibody

CaM Kinase IV (N-terminal region) Antibody

Cat. Num. AAA474250

• Gene Names: CAMK4; IV; caMK; CaMK IV; CaMK-GR
• Reactivity: Human, Rat, Mouse
• Applications: Western Blot, ELISA, Immunocytochemistry
• Synonyms: CaM Kinase IV; Monoclonal Antibody; CaM Kinase IV (N-terminal region) Antibody; CaM Kinase IV (N-terminal region); anti-CaM antibody

• Host: Mouse
• Reactivity: Human, Rat, Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: M299
• Specificity: This antibody detects a 60kDa* protein corresponding to the molecular mass of CaM Kinase IV on SDS-PAGE immunoblots of human Jurkat cells.
• Form/Format: Mouse monoclonal, protein A purified antibody is supplied in 100ul phosphate-buffered saline, 50% glycerol, 1mg/ml BSA, and 0.05% sodium azide.

• Applicable Applications for anti-CaM antibody: Western Blot (WB), ELISA (EIA), Immunocytochemistry (ICC)
• Application Notes: ELISA: 1:2000; ICC: 1:100; WB: 1:1000
• Immunogen: Clone (M299) was generated from a recombinant sequence that included amino acids in the N-terminal region of human CaM Kinase IV. This sequence has high homology to rat and mouse CaM Kinase IV.
• Preparation and Storage: Store at -20 degree C. Stable for 1 year.

Western Blot (WB)

(Western blot of human Jurkat cell lysate. The blot was probed with mouse monoclonal anti-CaM Kinase IV (N-terminal region) antibody at 1:250 (lane 1), 1:500 (lane 2), or 1:1000 (lane 3).)

Immunocytochemistry (ICC)

(Immunocytochemical labeling of CaM Kinase IV in aldehyde fixed and NP-40 permeabilized human NCI-H446 lung carcinoma cells. The cells were labeled with mouse monoclonal anti-CaM Kinase IV (CM2991). The antibody was detected using goat anti-mouse DyLight 594.)

Related Product Information for anti-CaM antibody

Hormones, growth factors and other cell signals cause increases in cytosolic Ca2+, which leads to cellular responses mediated by various Ca2+-binding proteins. Calmodulin (CaM) is a primary receptor for Ca2+ in cells. CaM functions as an allosteric activator of many enzymatic proteins including CaM kinases. CaM kinase IV is an important member of the CaM kinase family that has roles in neuronal long-term potentiation and memory, as well as T-cell receptor signaling. The Ca2+/calmodulin-dependent CaMKK phosphorylates CaM kinase IV, releasing the autoinhibitory effect and thus activating the kinase. The activated CaMKIV further autophosphorylates itself at Thr-200 to render the kinase constitutively active. The threonine phosphorylation state of CaMKIV can be downregulated by PP2A dephosphorylation.

References

Racioppi, L. & Means, A.R. (2008) Trends Immunol. 29(12):600.
Kang, H. et al. (2001) Cell 106:771.
Kasahara, J. et al. (2001) J. Biol. Chem. 276:24044.

NCBI and Uniprot Product Information

• NCBI GI #: 4502557
• NCBI GeneID: 814
• NCBI Accession #: NP_001735.1
• NCBI GenBank Nucleotide #: NM_001744.4
• UniProt Accession #: Q16566; D3DSZ7
• Molecular Weight: 51,926 Da
• NCBI Official Full Name: calcium/calmodulin-dependent protein kinase type IV
• NCBI Official Synonym Full Names: calcium/calmodulin-dependent protein kinase IV
• NCBI Official Symbol: CAMK4
• NCBI Official Synonym Symbols: IV; caMK; CaMK IV; CaMK-GR
• NCBI Protein Information: calcium/calmodulin-dependent protein kinase type IV; CAM kinase IV; caM kinase-GR; CAM kinase- GR; brain Ca++-calmodulin-dependent protein kinase type IV; brain Ca(2+)-calmodulin-dependent protein kinase type IV; calcium/calmodulin-dependent protein kinas
• UniProt Protein Name: Calcium/calmodulin-dependent protein kinase type IV
• UniProt Gene Name: CAMK4
• UniProt Synonym Gene Names: CAMK; CAMK-GR; CAMKIV; CaMK IV
• UniProt Entry Name: KCC4_HUMAN

NCBI Description

The product of this gene belongs to the serine/threonine protein kinase family, and to the Ca(2+)/calmodulin-dependent protein kinase subfamily. This enzyme is a multifunctional serine/threonine protein kinase with limited tissue distribution, that has been implicated in transcriptional regulation in lymphocytes, neurons and male germ cells. [provided by RefSeq, Jul 2008]

Uniprot Description

CAMK4: a protein kinase of the CAMK1 family. Implicated in transcriptional regulation in lymphocytes, neurons and male germ cells. Two alternatively sliced isoforms have been described.

Protein type: Protein kinase, CAMK; Kinase, protein; Protein kinase, Ser/Thr (non-receptor); EC 2.7.11.17; CAMK group; CAMK1 family

Chromosomal Location of Human Ortholog: 5q21.3

Cellular Component: nucleoplasm; nucleolus; cytosol

Molecular Function: calmodulin binding; calmodulin-dependent protein kinase activity; ATP binding

Biological Process: epidermal growth factor receptor signaling pathway; mitochondrion organization and biogenesis; fibroblast growth factor receptor signaling pathway; nerve growth factor receptor signaling pathway; organelle organization and biogenesis; positive regulation of transcription, DNA-dependent; regulation of T cell differentiation in the thymus; nucleocytoplasmic transport; myeloid dendritic cell differentiation; signal transduction; protein amino acid phosphorylation; positive regulation of protein export from nucleus; synaptic transmission; regulation of osteoclast differentiation; long-term memory; phospholipase C activation; innate immune response; nerve-nerve synaptic transmission; inflammatory response; myeloid dendritic cell cytokine production