Rabbit anti-Human GEMIN8 Polyclonal Antibody | anti-GEMIN8 antibody

GEMIN8, CT (GEMIN8, FAM51A1, Gem-associated protein 8, Protein FAM51A1) (Azide free) (HRP)

Cat. Num. AAA6301922

• Gene Names: GEMIN8; FAM51A1
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: GEMIN8; Polyclonal Antibody; CT (GEMIN8; FAM51A1; Gem-associated protein 8; Protein FAM51A1) (Azide free) (HRP); Protein FAM51A1; anti-GEMIN8 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-GEMIN8 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: GEMIN8 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 212-239 amino acids from the C-terminal region of human GEMIN8.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(GEMIN8 Antibody (C-term) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the GEMIN8 antibody detected the GEMIN8 protein (arrow).)

Related Product Information for anti-GEMIN8 antibody

The protein encoded by this gene is part of the SMN complex, which is necessary for spliceosomal snRNP assembly in the cytoplasm and pre-mRNA splicing in the nucleus. The encoded protein binds to both SMN1 and the GEMIN6/GEMIN7 heterodimer, mediating their interaction. This protein is found in nuclear Gemini of Cajal bodies (gems) and in the cytoplasm. Three transcript variants encoding the same protein have been found for this gene. [provided by RefSeq].

Product Categories/Family for anti-GEMIN8 antibody

Antibodies; Proteins

References

Carissimi, C., et al. J. Biol. Chem. 281(48):37009-37016(2006). Carissimi, C., et al. J. Biol. Chem. 281(12):8126-8134(2006).

NCBI and Uniprot Product Information

• NCBI GI #: 109715864
• NCBI GeneID: 54960
• NCBI Accession #: NP_001035944.1
• NCBI GenBank Nucleotide #: NM_001042479.1
• UniProt Accession #: Q9NWZ8; Q2LJ66; Q6ZV27; C4AMC4
• Molecular Weight: 28,637 Da
• NCBI Official Full Name: gem-associated protein 8
• NCBI Official Synonym Full Names: gem (nuclear organelle) associated protein 8
• NCBI Official Symbol: GEMIN8
• NCBI Official Synonym Symbols: FAM51A1
• NCBI Protein Information: gem-associated protein 8; gemin-8; family with sequence similarity 51, member A1
• UniProt Protein Name: Gem-associated protein 8
• Protein Family: Gem-associated protein
• UniProt Gene Name: GEMIN8
• UniProt Synonym Gene Names: FAM51A1; Gemin-8
• UniProt Entry Name: GEMI8_HUMAN

NCBI Description

The protein encoded by this gene is part of the SMN complex, which is necessary for spliceosomal snRNP assembly in the cytoplasm and pre-mRNA splicing in the nucleus. The encoded protein binds to both SMN1 and the GEMIN6/GEMIN7 heterodimer, mediating their interaction. This protein is found in nuclear Gemini of Cajal bodies (gems) and in the cytoplasm. Three transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, May 2010]

Uniprot Description

Function: The SMN complex plays an essential role in spliceosomal snRNP assembly in the cytoplasm and is required for pre-mRNA splicing in the nucleus. Ref.7

Subunit structure: Part of the core SMN complex that contains SMN1, GEMIN2/SIP1, DDX20/GEMIN3, GEMIN4, GEMIN5, GEMIN6, GEMIN7, GEMIN8 and STRAP/UNRIP. Binds directly to SMN1 and to the GEMIN6-GEMIN7 heterodimer, mediating their interaction. Ref.1

Subcellular location: Nucleus › gem. Cytoplasm. Note: Found in nuclear bodies called gems (Gemini of Cajal bodies) that are often in proximity to Cajal (coiled) bodies. Also found in the cytoplasm. Ref.1

Sequence caution: The sequence BAC86038.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.