Insulin Like Growth Factor 2 mRNA Binding Protein 3 (IGF2BP3) CLIA Kit | IGF2BP3 clia kit

CLIA Kit for Insulin Like Growth Factor 2 mRNA Binding Protein 3 (IGF2BP3)

Cat. Num. AAA2000512

• Gene Names: IGF2BP3; CT98; IMP3; KOC1; IMP-3; VICKZ3
• Synonyms: Insulin Like Growth Factor 2 mRNA Binding Protein 3 (IGF2BP3); CLIA Kit for Insulin Like Growth Factor 2 mRNA Binding Protein 3 (IGF2BP3); IGF2BP3 clia kit

• Specificity: This assay has high sensitivity and excellent specificity for detection of Insulin Like Growth Factor 2 mRNA Binding Protein 3 (IGF2BP3); No significant cross-reactivity or interference between Insulin Like Growth Factor 2 mRNA Binding Protein 3 (IGF2BP3) and analogues was observed.
• Sequence Length: 579

• Assay Type: Sandwich
• Samples: Tissue homogenates and other biological fluids
• Detection Range: 0.014-10ng/mL The standard curve concentrations used for the ELISA's were 10ng/mL, 3.33ng/mL, 1.11ng/mL, 0.37ng/mL, 0.123ng/mL, 0.041ng/mL, 0.014ng/mL
• Sensitivity: The minimum detectable dose of this kit is typically less than 0.061ng/mL
• Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Insulin Like Growth Factor 2 mRNA Binding Protein 3 (IGF2BP3) were tested 20 times on one plate, respectively.; Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Insulin Like Growth Factor 2 mRNA Binding Protein 3 (IGF2BP3) were tested on 3 different plates, 8 replicates in each plate.; CV(%) = SD/meanX100; Intra-Assay: CV<10%; Inter-Assay: CV<12%
• Organism Species: Rattus norvegicus (Rat)
• Assay Length: 4.5 hours
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for IGF2BP3 clia kit

The microtiter plate provided in this kit has been pre-coated with an antigen. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated secondary antibody. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Insulin Like Growth Factor 2 mRNA Binding Protein 3 (IGF2BP3) level in the sample or standard.

NCBI and Uniprot Product Information

• NCBI GI #: 30795212
• NCBI GeneID: 10643
• NCBI Accession #: NP_006538.2
• NCBI GenBank Nucleotide #: NM_006547.2
• UniProt Accession #: O00425; Q63HM0; Q6MZZ2; Q86VB1; A0A4Z5
• Molecular Weight: 63,705 Da
• NCBI Official Full Name: insulin-like growth factor 2 mRNA-binding protein 3
• NCBI Official Synonym Full Names: insulin-like growth factor 2 mRNA binding protein 3
• NCBI Official Symbol: IGF2BP3
• NCBI Official Synonym Symbols: CT98; IMP3; KOC1; IMP-3; VICKZ3
• NCBI Protein Information: insulin-like growth factor 2 mRNA-binding protein 3; hKOC; VICKZ family member 3; cancer/testis antigen 98; IGF2 mRNA-binding protein 3; IGF II mRNA binding protein 3; IGF-II mRNA-binding protein 3; KH domain containing protein overexpressed in cancer; KH domain-containing protein overexpressed in cancer
• UniProt Protein Name: Insulin-like growth factor 2 mRNA-binding protein 3
• Protein Family: Insulin-like growth factor 2 mRNA-binding protein
• UniProt Gene Name: IGF2BP3
• UniProt Synonym Gene Names: IMP3; KOC1; VICKZ3; IGF2 mRNA-binding protein 3; IMP-3; hKOC
• UniProt Entry Name: IF2B3_HUMAN

NCBI Description

The protein encoded by this gene is primarily found in the nucleolus, where it can bind to the 5' UTR of the insulin-like growth factor II leader 3 mRNA and may repress translation of insulin-like growth factor II during late development. The encoded protein contains several KH domains, which are important in RNA binding and are known to be involved in RNA synthesis and metabolism. A pseudogene exists on chromosome 7, and there are putative pseudogenes on other chromosomes. [provided by RefSeq, Jul 2008]

Uniprot Description

IGF2BP3: RNA-binding protein that act as a regulator of mRNA translation and stability. Binds to the 5'-UTR of the insulin-like growth factor 2 (IGF2) mRNAs. Binds to sequences in the 3'-UTR of CD44 mRNA. Belongs to the RRM IMP/VICKZ family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: RNA-binding; Spliceosome; Translation

Chromosomal Location of Human Ortholog: 7p11

Cellular Component: cytoplasm; cytosol; nucleus

Molecular Function: protein binding; mRNA 3'-UTR binding; RNA binding; nucleotide binding; translation regulator activity; mRNA 5'-UTR binding

Biological Process: anatomical structure morphogenesis; mRNA transport; translation; negative regulation of translation; gene expression; regulation of cytokine biosynthetic process