Rabbit anti-Human, Mouse INMT Polyclonal Antibody | anti-INMT antibody

INMT, ID (INMT, Indolethylamine N-methyltransferase, Aromatic alkylamine N-methyltransferase, Thioether S-methyltransferase)

Cat. Num. AAA646939

• Gene Names: INMT; TEMT
• Reactivity: Human, Mouse
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Affinity Purified; Purified by Protein A affinity chromatography.
• Synonyms: INMT; Polyclonal Antibody; ID (INMT; Indolethylamine N-methyltransferase; Aromatic alkylamine N-methyltransferase; Thioether S-methyltransferase); Anti -INMT; anti-INMT antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Human, mouse
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.

• Applicable Applications for anti-INMT antibody: ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in Western Blot, Immunohistochemistry, ELISA; Dilution: ELISA: 1:1,000; Western Blot: 1:100-500; Immunohistochemistry: 1:50-100
• Immunogen: INMT antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 108-137 amino acids from the Central region of human INMT.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(INMT Antibody (Center) western blot analysis in mouse heart tissue lysates (35ug/lane).This demonstrates the INMT antibody detected INMT protein (arrow).)

Immunohistochemistry (IHC)

(INMT Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the INMT Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)

Related Product Information for anti-INMT antibody

N-methylation of endogenous and xenobiotic compounds is a major method by which they are degraded. This gene encodes an enzyme that N-methylates indoles such as tryptamine. [provided by RefSeq].

Product Categories/Family for anti-INMT antibody

Antibodies; Abs to Enzymes; Methyltransferase

NCBI and Uniprot Product Information

• NCBI GI #: 75515647
• NCBI GeneID: 11185
• UniProt Accession #: O95050; Q3KP49; Q9P1Y2; Q9UBY4; Q9UHQ0; B8ZZ69
• Molecular Weight: 28,891 Da
• NCBI Official Full Name: INMT protein, partial
• NCBI Official Synonym Full Names: indolethylamine N-methyltransferase
• NCBI Official Symbol: INMT
• NCBI Official Synonym Symbols: TEMT
• NCBI Protein Information: indolethylamine N-methyltransferase; nicotine N-methyltransferase; arylamine N-methyltransferase; thioether S-methyltransferase; indolamine N-methyltransferase; aromatic alkylamine N-methyltransferase
• UniProt Protein Name: Indolethylamine N-methyltransferase
• Protein Family: Indolethylamine N-methyltransferase
• UniProt Gene Name: INMT
• UniProt Synonym Gene Names: Indolamine N-methyltransferase; Amine N-methyltransferase; Arylamine N-methyltransferase; TEMT
• UniProt Entry Name: INMT_HUMAN

NCBI Description

N-methylation of endogenous and xenobiotic compounds is a major method by which they are degraded. This gene encodes an enzyme that N-methylates indoles such as tryptamine. Alternative splicing results in multiple transcript variants. Read-through transcription also exists between this gene and the downstream FAM188B (family with sequence similarity 188, member B) gene. [provided by RefSeq, Nov 2010]

Uniprot Description

Function: Functions as thioether S-methyltransferase and is active with a variety of thioethers and the corresponding selenium and tellurium compounds, including 3-methylthiopropionaldehyde, dimethyl selenide, dimethyl telluride, 2-methylthioethylamine, 2-methylthioethanol, methyl-n-propyl sulfide and diethyl sulfide. Plays an important role in the detoxification of selenium compounds

By similarity. Catalyzes the N-methylation of tryptamine and structurally related compounds. Ref.1

Catalytic activity: S-adenosyl-L-methionine + an amine = S-adenosyl-L-homocysteine + a methylated amine. Ref.1S-adenosyl-L-methionine + dimethyl sulfide = S-adenosyl-L-homocysteine + trimethylsulfonium. Ref.1

Subunit structure: Monomer

By similarity.

Subcellular location: Cytoplasm

By similarity.

Tissue specificity: Widely expressed. The highest levels were in thyroid, adrenal gland, adult and fetal lung. Intermediate levels in heart, placenta, skeletal muscle, testis, small intestine, pancreas, stomach, spinal cord, lymph node and trachea. Very low levels in adult and fetal kidney and liver, in adult spleen, thymus, ovary, colon and bone marrow. Not expressed in peripheral blood leukocytes and brain. Ref.1

Sequence similarities: Belongs to the class I-like SAM-binding methyltransferase superfamily. NNMT/PNMT/TEMT family.

Biophysicochemical propertiesKinetic parameters:KM=2.9 mM for tryptamine Ref.1