Mouse anti-Human TAF15 RNA Polymerase II, TATA Box Binding Protein Monoclonal Antibody | anti-TAF15 antibody

TAF15 RNA Polymerase II, TATA Box Binding Protein (TBP)-associated Factor, 68kD (TAF15, RBP56, TAF2N, TAFII68, hTAFII68)

Cat. Num. AAA602701

• Gene Names: TAF15; Npl3; RBP56; TAF2N; TAFII68; hTAFII68
• Reactivity: Human
• Applications: Western Blot
• Purity: Affinity Purified; Purified by Protein G affinity chromatography.
• Synonyms: TAF15 RNA Polymerase II; TATA Box Binding Protein; Monoclonal Antibody; TATA Box Binding Protein (TBP)-associated Factor; 68kD (TAF15; RBP56; TAF2N; TAFII68; hTAFII68); Anti -TAF15 RNA Polymerase II; anti-TAF15 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 8C319
• Specificity: Recognizes human TAF15 RNA Polymerase II, TATA Box Binding Protein (TBP)-associated Factor, 68kD.
• Purity/Purification: Affinity Purified; Purified by Protein G affinity chromatography.
• Form/Format: Supplied as a sterile-filtered liquid in PBS, pH 7.4, 1% BSA, 0.05% sodium azide.

• Applicable Applications for anti-TAF15 antibody: Western Blot (WB)
• Application Notes: Suitable for use in Western Blot.
• Immunogen: Partial sequence of recombinant full-length protein to human TAF15 RNA Polymerase II, TATA Box Binding Protein (TBP)-associated Factor, 68kD
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Western Blot (WB)

(Western blot analysis of immunized recombinant protein, using MBS602701.)

Immunostaining (IS)

(Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100 in PBS. The cells were immunostained with MBS602701)

Related Product Information for anti-TAF15 antibody

Initiation of transcription by RNA polymerase II requires the activities of more than 70 polypeptides. The protein that coordinates these activities is transcription factor IID (TFIID), which binds to the core promoter to position the polymerase properly, serves as the scaffold for assembly of the remainder of the transcription complex, and acts as a channel for regulatory signals. TFIID is composed of the TATA-binding protein (TBP) and a group of evolutionarily conserved proteins known as TBP-associated factors or TAFs. TAFs may participate in basal transcription, serve as coactivators, function in promoter recognition or modify general transcription factors (GTFs) to facilitate complex assembly and transcription initiation. This gene encodes a subunit of TFIID present in a subset of TFIID complexes. Translocations involving chromosome 17 and chromosome 9, where the gene for the nuclear receptor CSMF is located, result in a gene fusion product that is an RNA binding protein associated with a subset of extraskeletal myxoid chondrosarcomas. Two transcripts encoding different isoforms have been identified.

Product Categories/Family for anti-TAF15 antibody

Antibodies; Abs to Enzymes; Polymerase

NCBI and Uniprot Product Information

• NCBI GI #: 119600533
• NCBI GeneID: 8148
• UniProt Accession #: Q86X94; Q92804
• Molecular Weight: 61,830 Da
• NCBI Official Full Name: TAF15 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 68kDa, isoform CRA_d
• NCBI Official Synonym Full Names: TAF15 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 68kDa
• NCBI Official Symbol: TAF15
• NCBI Official Synonym Symbols: Npl3; RBP56; TAF2N; TAFII68; hTAFII68
• NCBI Protein Information: TATA-binding protein-associated factor 2N; TAF(II)68; RBP56/CSMF fusion; TBP-associated factor 15; 68 kDa TATA-binding protein-associated factor; TATA box-binding protein-associated factor 2N (RNA-binding protein 56); TATA box binding protein (TBP)-associated factor, RNA polymerase II, N, 68kD (RNA-binding protein 56)
• UniProt Protein Name: TAF15 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 68kDa
• Protein Family: Transcription initiation factor
• UniProt Gene Name: TAF15
• UniProt Entry Name: Q86X94_HUMAN

NCBI Description

Initiation of transcription by RNA polymerase II requires the activities of more than 70 polypeptides. The protein that coordinates these activities is transcription factor IID (TFIID), which binds to the core promoter to position the polymerase properly, serves as the scaffold for assembly of the remainder of the transcription complex, and acts as a channel for regulatory signals. TFIID is composed of the TATA-binding protein (TBP) and a group of evolutionarily conserved proteins known as TBP-associated factors or TAFs. TAFs may participate in basal transcription, serve as coactivators, function in promoter recognition or modify general transcription factors (GTFs) to facilitate complex assembly and transcription initiation. This gene encodes a subunit of TFIID present in a subset of TFIID complexes. Translocations involving chromosome 17 and chromosome 9, where the gene for the nuclear receptor CSMF is located, result in a gene fusion product that is an RNA binding protein associated with a subset of extraskeletal myxoid chondrosarcomas. Two transcripts encoding different isoforms have been identified. [provided by RefSeq]

Uniprot Description

TAF15: RNA and ssDNA-binding protein that may play specific roles during transcription initiation at distinct promoters. Can enter the preinitiation complex together with the RNA polymerase II (Pol II). A chromosomal aberration involving TAF15/TAF2N is found in a form of extraskeletal myxoid chondrosarcomas (EMC). Translocation t(9;17)(q22;q11) with NR4A3. Belongs to the RRM TET family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: RNA-binding; Oncoprotein; Translation

Chromosomal Location of Human Ortholog: 17q11.1-q11.2

Cellular Component: nucleoplasm; cytoplasm; nucleolus; nucleus

Molecular Function: protein binding; DNA binding; zinc ion binding; nucleotide binding

Biological Process: positive regulation of transcription, DNA-dependent

Disease: Chondrosarcoma, Extraskeletal Myxoid