Rabbit anti-Human SF3B4 Polyclonal Antibody | anti-SF3B4 antibody

SF3B4, ID (SF3B4, SAP49, Splicing factor 3B subunit 4, Pre-mRNA-splicing factor SF3b 49kD subunit, SF3b50, Spliceosome-associated protein 49) (PE)

Cat. Num. AAA6346569

• Gene Names: SF3B4; AFD1; Hsh49; SAP49; SF3b49
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: SF3B4; Polyclonal Antibody; ID (SF3B4; SAP49; Splicing factor 3B subunit 4; Pre-mRNA-splicing factor SF3b 49kD subunit; SF3b50; Spliceosome-associated protein 49) (PE); Spliceosome-associated protein 49; anti-SF3B4 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-SF3B4 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: SF3B4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 157-187 amino acids from the Central region of human SF3B4.
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(SF3B4 Antibody (Center) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the SF3B4 antibody detected the SF3B4 protein (arrow).)

Related Product Information for anti-SF3B4 antibody

This gene encodes one of four subunits of the splicing factor 3B. The protein encoded by this gene cross-links to a region in the pre-mRNA immediately upstream of the branchpoint sequence in pre-mRNA in the prespliceosomal complex A. It also may be involved in the assembly of the B, C and E spliceosomal complexes. In addition to RNA-binding activity, this protein interacts directly and highly specifically with subunit 2 of the splicing factor 3B. This protein contains two N-terminal RNA-recognition motifs (RRMs), consistent with the observation that it binds directly to pre-mRNA.

Product Categories/Family for anti-SF3B4 antibody

Antibodies; Splicing Factors

References

Gudbjartsson, D.F., et al. Nat. Genet. 40(5):609-615(2008). Rikova, K., et al. Cell 131(6):1190-1203(2007). Watanabe, H., et al. J. Biol. Chem. 282(28):20728-20738(2007). Wu, C., et al. Proteomics 7(11):1775-1785(2007). Ewing, R.M., et al. Mol. Syst. Biol. 3, 89 (2007).

NCBI and Uniprot Product Information

• NCBI GI #: 5032069
• NCBI GeneID: 10262
• NCBI Accession #: NP_005841.1
• NCBI GenBank Nucleotide #: NM_005850.4
• UniProt Accession #: Q15427; Q5SZ63
• Molecular Weight: 44,386 Da
• NCBI Official Full Name: splicing factor 3B subunit 4
• NCBI Official Synonym Full Names: splicing factor 3b, subunit 4, 49kDa
• NCBI Official Symbol: SF3B4
• NCBI Official Synonym Symbols: AFD1; Hsh49; SAP49; SF3b49
• NCBI Protein Information: splicing factor 3B subunit 4; SAP 49; SF3b50; spliceosomal protein; spliceosome-associated protein 49; spliceosome-associated protein (U2 snRNP); pre-mRNA-splicing factor SF3b 49 kDa subunit
• UniProt Protein Name: Splicing factor 3B subunit 4
• Protein Family: Splicing factor
• UniProt Gene Name: SF3B4
• UniProt Synonym Gene Names: SAP49; SAP 49
• UniProt Entry Name: SF3B4_HUMAN

NCBI Description

This gene encodes one of four subunits of the splicing factor 3B. The protein encoded by this gene cross-links to a region in the pre-mRNA immediately upstream of the branchpoint sequence in pre-mRNA in the prespliceosomal complex A. It also may be involved in the assembly of the B, C and E spliceosomal complexes. In addition to RNA-binding activity, this protein interacts directly and highly specifically with subunit 2 of the splicing factor 3B. This protein contains two N-terminal RNA-recognition motifs (RRMs), consistent with the observation that it binds directly to pre-mRNA. [provided by RefSeq, Jul 2008]

Uniprot Description

SF3B4: subunit of the splicing factor SF3B required for 'A' complex assembly formed by the stable binding of U2 snRNP to the branchpoint sequence (BPS) in pre-mRNA. Sequence independent binding of SF3A/SF3B complex upstream of the branch site is essential, it may anchor U2 snRNP to the pre-mRNA. May also be involved in the assembly of the 'E' complex. SF3B4 has been found in complex 'B' and 'C' as well. Belongs also to the minor U12-dependent spliceosome, which is involved in the splicing of rare class of nuclear pre-mRNA intron.

Protein type: RNA-binding; Spliceosome; RNA splicing

Chromosomal Location of Human Ortholog: 1q21.2

Cellular Component: nucleoplasm; spliceosome; U12-dependent spliceosome

Molecular Function: protein binding; nucleotide binding

Biological Process: RNA splicing, via transesterification reactions; nuclear mRNA splicing, via spliceosome; positive regulation of nuclear mRNA splicing, via spliceosome; RNA splicing; gene expression; mRNA processing

Disease: Acrofacial Dysostosis 1, Nager Type