Mouse BrdU (bromodeoxyuridine) Monoclonal Antibody
BrdU (bromodeoxyuridine) FITC Conjugate
Reactivity
BrdU Incorporated Cells
Applications
Immunohistochemistry, Flow Cytometry, Functional Assay
Purity
Protein A/G Chromatography
Synonyms
BrdU (bromodeoxyuridine); Monoclonal Antibody; BrdU (bromodeoxyuridine) FITC Conjugate; anti-BrdU (bromodeoxyuridine) antibody
Host
Mouse
Reactivity
BrdU Incorporated Cells
Clonality
Monoclonal
Isotype
IgG1
Clone Number
MoBU-1
Purity/Purification
Protein A/G Chromatography
Form/Format
Provided as solution in phosphate buffered saline with 0.08% sodium azide
Applicable Applications for anti-BrdU (bromodeoxyuridine) antibody
Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
Immunogen
Bromodeoxyuridine conjugated to Helix Pomatia Haemocyanin used to immunize BALB/c mice
Preparation and Storage
Product should be stored at -20 degree C. Aliquot to avoid freeze/thaw cycles
Related Product Information for anti-BrdU (bromodeoxyuridine) antibody
Bromodexyuridine (BrdU) is a thymidine analog which is selectively incorporated into the DNA of proliferating cells to provide a marker for the DNA being replicated. The number of proliferating cells can then be detected in cell lysates, tissue sections or suspensions using an antibody specific for the BrdU. Previous methods of detecting DNA included the use of [3H]-thymidine which would be incorporated into the DNA and could then the DNA could be quantified by autoradiography or scintillation counting. These methods are more difficult and require more cleanup due to the radioactive material. An immunohistochemical assay provides a much simpler and cleaner method for detecting DNA in cells. This antibody is also useful for detecting proliferating cells by flow cytometry or immunofluorescence staining. The FITC conjugate is particularly useful in these applications as a secondary antibody is not necessary for visualization. Also, a specific anti-FITC antibody conjugate can be used for signal amplification if desired.
Product Categories/Family for anti-BrdU (bromodeoxyuridine) antibody
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Product Notes
The BrdU (bromodeoxyuridine) (Catalog #AAA395326) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The BrdU (bromodeoxyuridine) FITC Conjugate reacts with BrdU Incorporated Cells and may cross-react with other species as described in the data sheet. AAA Biotech's BrdU (bromodeoxyuridine) can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC), Flow Cytometry (FC/FACS). Researchers should empirically determine the suitability of the BrdU (bromodeoxyuridine) for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "BrdU (bromodeoxyuridine), Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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