Western Blot (WB)
(Western blot analysis of exBrachyuryracBrachyurys of H460 cells, using Brachyury Rabbit pAb aBrachyury 1:1000 diluBrachyuryion.Secondary anBrachyuryibody: HRP GoaBrachyury AnBrachyuryi-RabbiBrachyury IgG (H+L) (AS014) aBrachyury 1:10000 diluBrachyuryion.LysaBrachyuryes/proBrachyuryeins: 25ug per lane.Blocking buffer: 3% nonfaBrachyury dry milk in BrachyuryBSBrachyury.DeBrachyuryecBrachyuryion: ECL Basic KiBrachyury (RM00020).Exposure Brachyuryime: 30s.)
Rabbit anti-Human, Rat Brachyury Polyclonal Antibody | anti-T antibody
Brachyury Rabbit pAb
IF: 1:50-1:200
Western Blot (WB)
(Western blot analysis of exBrachyuryracBrachyurys of H460 cells, using Brachyury Rabbit pAb aBrachyury 1:1000 diluBrachyuryion.Secondary anBrachyuryibody: HRP GoaBrachyury AnBrachyuryi-RabbiBrachyury IgG (H+L) (AS014) aBrachyury 1:10000 diluBrachyuryion.LysaBrachyuryes/proBrachyuryeins: 25ug per lane.Blocking buffer: 3% nonfaBrachyury dry milk in BrachyuryBSBrachyury.DeBrachyuryecBrachyuryion: ECL Basic KiBrachyury (RM00020).Exposure Brachyuryime: 30s.)
Western Blot (WB)
(Western blot analysis of exBrachyuryracBrachyurys of H460 cells, using Brachyury Rabbit pAb aBrachyury 1:1000 diluBrachyuryion.Secondary anBrachyuryibody: HRP GoaBrachyury AnBrachyuryi-RabbiBrachyury IgG (H+L) (AS014) aBrachyury 1:10000 diluBrachyuryion.LysaBrachyuryes/proBrachyuryeins: 25ug per lane.Blocking buffer: 3% nonfaBrachyury dry milk in BrachyuryBSBrachyury.DeBrachyuryecBrachyuryion: ECL Basic KiBrachyury (RM00020).Exposure Brachyuryime: 30s.)
Immunofluorescence (IF)
(Immunofluorescence analysis of NCI-H460 cells using Brachyury Rabbit pAb aBrachyury diluBrachyuryion of 1:200 (40x lens). Blue: DAPI for nuclear sBrachyuryaining.)
Immunofluorescence (IF)
(Immunofluorescence analysis of NCI-H460 cells using Brachyury Rabbit pAb aBrachyury diluBrachyuryion of 1:200 (40x lens). Blue: DAPI for nuclear sBrachyuryaining.)
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is an embryonic nuclear transcription factor that binds to a specific DNA element, the palindromic T-site. It binds through a region in its N-terminus, called the T-box, and effects transcription of genes required for mesoderm formation and differentiation. The protein is localized to notochord-derived cells. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2012]
Uniprot Description
Brachyury: Involved in the transcriptional regulation of genes required for mesoderm formation and differentiation. Binds to a palindromic site (called T site) and activates gene transcription when bound to such a site. Genetic variations in T are associated with susceptibility to neural tube defects (NTD). NTD are common congenital malformations. Spina bifida, which results from malformations in the caudal region of the neural tube, is compatible with life but associated with significant morbidity, including lower limb paralysis. T is involved in susceptibility to the development of chordoma (CHDM). Chordomas are rare, clinically malignant tumors derived from notochordal remnants. They occur along the length of the spinal axis, predominantly in the sphenooccipital, vertebral and sacrococcygeal regions. They are characterized by slow growth, local destruction of bone, extension into adjacent soft tissues and rarely, distant metastatic spread. Susceptibility to development of chordomas is due to a T gene duplication.
Protein type: Motility/polarity/chemotaxis; DNA-binding; Cell cycle regulation; Transcription factor
Chromosomal Location of Human Ortholog: 6q27
Cellular Component: cytoplasm; nuclear chromatin; nucleus
Molecular Function: RNA polymerase II transcription factor activity, enhancer binding; transcription factor activity
Biological Process: transcription from RNA polymerase II promoter; heart morphogenesis; somitogenesis; neural plate morphogenesis; embryonic skeletal development; mesoderm migration; notochord formation; negative regulation of transcription from RNA polymerase II promoter; Wnt receptor signaling pathway through beta-catenin; signal transduction; BMP signaling pathway; determination of anterior/posterior axis, embryo; positive regulation of cell proliferation; neural tube closure; penetration of zona pellucida; mesoderm development; vasculogenesis
Disease: Neural Tube Defects; Sacral Agenesis With Vertebral Anomalies
Research Articles on T
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Product Notes
The T t (Catalog #AAA9142502) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Brachyury Rabbit pAb reacts with Human, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Brachyury can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF). WB: 1:500-1:1000 IF: 1:50-1:200. Researchers should empirically determine the suitability of the T t for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: HKEMMEEPGD SQQPGYSQWG WLLPGTSTLC PPANPHPQFG GALSLPSTHS CDRYPTLRSH RSSPYPSPYA HRNNSPTYSD NSPACLSMLQ SHDNWSSLGM PAHPSMLPVS HNASPPTSSS QYPSLWSVSN GAVTPGSQAA AVSNGLGAQF FRGSPAHYTP LTHPVSAPSS SGSPLYEGAA AATDIVDSQY DAAAQGRLIA SWTPVSPPSM. It is sometimes possible for the material contained within the vial of "Brachyury, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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