Immunofluorescence (IF)
(Immunofluorescence staining of COMMD9 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with mouse anti-human COMMD9 monoclonal antibody (dilution ratio 1:60) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-mouse IgG secondary antibody (green). Positive staining was localized to Cytoplasm and nucleus.)
Mouse anti-Human COMMD9 Monoclonal Antibody | anti-COMMD9 antibody
Anti-COMMD9 Antibody, Mouse Monoclonal
Gene Names
COMMD9; HSPC166
Reactivity
Human
Applications
ELISA, Immunocytochemistry, Immunofluorescence
Purity
Protein A
Synonyms
COMMD9; Monoclonal Antibody; Anti-COMMD9 Antibody; Mouse Monoclonal; COMMD9 Antibody; Mouse MAb; COMM domain containing 9; Anti-HSPC166 Antibody; anti-COMMD9 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
Mouse IgG2a
Clone Number
04
Specificity
Human COMMD9
Purity/Purification
Protein A
Form/Format
Liquid; 0.2um filtered solution in PBS
Applicable Applications for anti-COMMD9 antibody
ELISA (EIA), Immunocytochemistry (ICC), Immunofluorescence (IF)
Application Notes
ELISA: 1:1000-1:2000
ICC/IF: 1:20-1:100
ICC/IF: 1:20-1:100
Immunogen
Recombinant Human COMMD9 Protein
Conjugation
Unconjugated
Preparation
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human COMMD9 (rh COMMD9). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.
Immunofluorescence (IF)
(Immunofluorescence staining of COMMD9 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with mouse anti-human COMMD9 monoclonal antibody (dilution ratio 1:60) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-mouse IgG secondary antibody (green). Positive staining was localized to Cytoplasm and nucleus.)
Immunofluorescence (IF)
(Immunofluorescence staining of COMMD9 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with mouse anti-human COMMD9 monoclonal antibody (dilution ratio 1:60) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-mouse IgG secondary antibody (green). Positive staining was localized to Cytoplasm and nucleus.)
Related Product Information for anti-COMMD9 antibody
COMMD9 is a COMM domain-containing or COMMD protein. COMMD family is comprised of ten members which are widely conserved throughout evolution and share certain functional properties. They represent a recently discovered set of evolutionarily conserved factors characterized by the presence of a defining carboxy-terminal motif. COMMD protein functions in the control of the transcription factor NFkappaB. NFkappaB plays a critical role in a number of homeostatic processes in multicellular organisms, including the regulation of immunity and cell survival. COMMD proteins inhibit NFkappaB mediated gene expression, and recent mechanistic studies have revealed that COMMD1 controls the ubiquitination of NFkappaB subunits, an event linked to transcriptional termination. COMMD1 binds to a multimeric ubiquitin ligase containing Elongins B/C, Cul2 and SOCS1 (ECS(SOCS1)). In this complex, COMMD1 facilitates the binding of NFkappaB subunits to the ligase, thereby promoting their ubiquitination and degradation. Additional insights gained from these studies indicate that COMMD proteins likely play a broader role in cellular homeostasis through their participation in the ubiquitination pathway.
References
Ota T. et al., 2004, Nat Genet. 36 (1): 40-5. Gerhard DS. et al., 2004, Genome Res. 14 (10B): 2121-7. Burstein E. et al., 2005, J Biol Chem. 280 (23): 22222-32.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
17,333 Da
NCBI Official Full Name
COMM domain-containing protein 9 isoform 1
NCBI Official Synonym Full Names
COMM domain containing 9
NCBI Official Symbol
COMMD9
NCBI Official Synonym Symbols
HSPC166
NCBI Protein Information
COMM domain-containing protein 9
UniProt Protein Name
COMM domain-containing protein 9
Protein Family
UniProt Gene Name
COMMD9
UniProt Entry Name
COMD9_HUMAN
Uniprot Description
COMMD9: 2 isoforms of the human protein are produced by alternative splicing.
Chromosomal Location of Human Ortholog: 11p13
Cellular Component: cytoplasmic membrane-bound vesicle; nucleus
Molecular Function: protein binding
Biological Process: regulation of transcription, DNA-dependent; transcription, DNA-dependent; sodium ion transport
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Product Notes
The COMMD9 commd9 (Catalog #AAA8107420) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Anti-COMMD9 Antibody, Mouse Monoclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's COMMD9 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Immunocytochemistry (ICC), Immunofluorescence (IF). ELISA: 1:1000-1:2000 ICC/IF: 1:20-1:100. Researchers should empirically determine the suitability of the COMMD9 commd9 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "COMMD9, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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