Mouse anti-Mouse, Rat AcCoA Carboxylase Monoclonal Antibody | anti-Acly antibody

AcCoA Carboxylase (Ser79), phosphorylated (Azide Free)

Cat. Num. AAA602195

• Gene Names: Acly; AW538652; A730098H14Rik
• Reactivity: Mouse, Rat
• Applications: Western Blot
• Purity: Purified; Purified by Protein G affinity chromatography.
• Synonyms: AcCoA Carboxylase; Monoclonal Antibody; AcCoA Carboxylase (Ser79); phosphorylated (Azide Free); Anti -AcCoA Carboxylase (Ser79); anti-Acly antibody

• Host: Mouse
• Reactivity: Mouse, Rat
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 3J1
• Specificity: Recognizes phosphorylated rat Acetyl CoA Carboxylase (Ser79), Mr 257kD. Species Crossreactivity: mouse.
• Purity/Purification: Purified; Purified by Protein G affinity chromatography.
• Form/Format: Supplied as a liquid in PBS. No stabilizing proteins added. No preservative added. Glycerol-free. Also available with azide. See A0225-05A.

• Applicable Applications for anti-Acly antibody: Western Blot (WB)
• Application Notes: Suitable for use in Western Blot.; Dilution: Western Blot: 0.5-2ug/ml. Detects phospho-Acetyl CoA Carboxylase (Ser79) in mouse heart cytosolic lysates. Mouse heart cytosolic lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with A0225-05A. Proteins were visualized using an IgG (HRP) goat anti-mouse and a chemiluminescence detection system.
• Immunogen: Synthetic peptide containing (…SSM[pS]GL), in which [pS] corresponds to phospho-Serine at residue 79 of rat Acetyl CoA Carboxylase (KLH).
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(WB: Mouse heart cytosolic lysate was probed with MBS602195)

Related Product Information for anti-Acly antibody

Acetyl-CoA carboxylase (ACC) is a complex multifunctional enzyme system. ACC is a biotin-containing enzyme which catalyzes the carboxylation of acetyl-CoA to malonyl-CoA, the rate-limiting step in fatty acid synthesis. There are two ACC forms, alpha and beta, encoded by two different genes. ACC-alpha is highly enriched in lipogenic tissues. The enzyme is under long term control at the transcriptional and translational levels and under short term regulation by the phosphorylation/dephosphorylation of targeted serine residues and by allosteric transformation by citrate or palmitoyl-CoA. Multiple alternatively spliced transcript variants divergent in the 5' sequence and encoding distinct isoforms have been found for this gene.

Product Categories/Family for anti-Acly antibody

Antibodies; Abs to Enzymes

NCBI and Uniprot Product Information

• NCBI GI #: 313151222
• NCBI GeneID: 104112
• NCBI Accession #: NP_001186225.1
• NCBI GenBank Nucleotide #: NM_001199296.1
• UniProt Accession #: Q91V92
• Molecular Weight: 119,728 Da
• NCBI Official Full Name: ATP-citrate synthase isoform 1
• NCBI Official Synonym Full Names: ATP citrate lyase
• NCBI Official Symbol: Acly
• NCBI Official Synonym Symbols: AW538652; A730098H14Rik
• NCBI Protein Information: ATP-citrate synthase; citrate cleavage enzyme; ATP-citrate (pro-S-)-lyase
• UniProt Protein Name: ATP-citrate synthase
• Protein Family: ATP-citrate synthase
• UniProt Gene Name: Acly
• UniProt Entry Name: ACLY_MOUSE

Uniprot Description

ACLY: ATP citrate-lyase is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA in many tissues. Has a central role in de novo lipid synthesis. In nervous tissue it may be involved in the biosynthesis of acetylcholine. Homotetramer. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Carbohydrate Metabolism - citrate (TCA) cycle; Transferase; Lyase; EC 2.3.3.8

Cellular Component: nucleoplasm; mitochondrion; membrane; cytoplasm; plasma membrane; intracellular

Molecular Function: transferase activity; protein binding; transferase activity, transferring acyl groups, acyl groups converted into alkyl on transfer; ATP citrate synthase activity; metal ion binding; nucleotide binding; succinate-CoA ligase (ADP-forming) activity; cofactor binding; catalytic activity; ATP binding

Biological Process: acetyl-CoA metabolic process; metabolic process; lipid biosynthetic process; acetyl-CoA biosynthetic process; citrate metabolic process; cellular carbohydrate metabolic process; lipid metabolic process; fatty acid biosynthetic process