Rabbit anti-Human Bak, BH3 Domain Polyclonal Antibody | anti-BAK1 antibody

Bak, BH3 Domain (Bcl-2 Homologous Antagonist/Killer, Apoptosis Regulator BAK, Bcl-2-like Protein 7, Bcl2-L-7, BAK1, BCL2L7, CDN1)

Cat. Num. AAA623426

• Gene Names: BAK1; BAK; CDN1; BCL2L7; BAK-LIKE
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Affinity Purified; Purified by Protein G affinity chromatography.
• Synonyms: Bak; BH3 Domain; Polyclonal Antibody; BH3 Domain (Bcl-2 Homologous Antagonist/Killer; Apoptosis Regulator BAK; Bcl-2-like Protein 7; Bcl2-L-7; BAK1; BCL2L7; CDN1); Anti -Bak; anti-BAK1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human Bak.
• Purity/Purification: Affinity Purified; Purified by Protein G affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, 0.09% sodium azide.

• Applicable Applications for anti-BAK1 antibody: ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in ELISA, Western Blot, and Immunohistochemistry.; Dilution: ELISA: 1:1,000; Western Blot: 1:100-1:500; Immunohistochemistry: 1:50-1:100
• Immunogen: Synthetic peptide selected from the N-terminal region (BH3 domain) of human Bak (KLH).
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Immunohistochemistry (IHC)

(Immunohistochemistry analysis in formalin-fixed and paraffin-embedded human cancer tissue reacted with MBS623426, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry. BC = breast carcinoma; HC = hepatocarcinoma.)

Western Blot (WB)

(Western Blot analysis in Jurkat, MDA-MB453, 293 cell line lysates (35ug/lane) using MBS623426. This demonstrates that MBS623426 detected the Bak protein (arrow).)

Related Product Information for anti-BAK1 antibody

BAK belongs to the BCL2 protein family. BCL2 family members form oligomers or heterodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. BAK localizes to mitochondria, and functions to induce apoptosis. It interacts with and accelerates the opening of the mitochondrial voltage-dependent anion channel, which leads to a loss in membrane potential and the release of cytochrome c. This protein also interacts with the tumor suppressor P53 after exposure to cell stress.

Product Categories/Family for anti-BAK1 antibody

Antibodies; Abs to Apoptosis

NCBI and Uniprot Product Information

• NCBI GI #: 4502363
• NCBI GeneID: 578
• NCBI Accession #: NP_001179.1
• NCBI GenBank Nucleotide #: NM_001188.3
• UniProt Accession #: Q16611; Q6I9T6; Q92533
• Molecular Weight: 23,409 Da
• NCBI Official Full Name: bcl-2 homologous antagonist/killer
• NCBI Official Synonym Full Names: BCL2-antagonist/killer 1
• NCBI Official Symbol: BAK1
• NCBI Official Synonym Symbols: BAK; CDN1; BCL2L7; BAK-LIKE
• NCBI Protein Information: bcl-2 homologous antagonist/killer; bcl2-L-7; BCL2-like 7 protein; bcl-2-like protein 7; apoptosis regulator BAK; pro-apoptotic protein BAK
• UniProt Protein Name: Bcl-2 homologous antagonist/killer
• Protein Family: Bcl-2 homologous antagonist/killer
• UniProt Gene Name: BAK1
• UniProt Synonym Gene Names: BAK; BCL2L7; CDN1; Bcl2-L-7
• UniProt Entry Name: BAK_HUMAN

NCBI Description

The protein encoded by this gene belongs to the BCL2 protein family. BCL2 family members form oligomers or heterodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. This protein localizes to mitochondria, and functions to induce apoptosis. It interacts with and accelerates the opening of the mitochondrial voltage-dependent anion channel, which leads to a loss in membrane potential and the release of cytochrome c. This protein also interacts with the tumor suppressor P53 after exposure to cell stress. [provided by RefSeq, Jul 2008]

Uniprot Description

BAK1: In the presence of an appropriate stimulus, accelerates programmed cell death by binding to, and antagonizing the anti- apoptotic action of BCL2 or its adenovirus homolog E1B 19k protein. Low micromolar levels of zinc ions inhibit the promotion of apoptosis. Interacts with BCL2A1. Homodimer. Formation of the homodimer is zinc-dependent. Forms heterodimers with BCL2, E1B 19k protein, and BCL2L1 isoform Bcl-X(L). Interacts with myxoma virus protein M11L. Expressed in a wide variety of tissues, with highest levels in the heart and skeletal muscle. Belongs to the Bcl-2 family.

Protein type: Mitochondrial; Apoptosis; Endoplasmic reticulum; Membrane protein, integral

Chromosomal Location of Human Ortholog: 6p21.3

Cellular Component: pore complex; mitochondrial outer membrane; mitochondrion; endoplasmic reticulum; cytosol; integral to mitochondrial outer membrane

Molecular Function: identical protein binding; BH domain binding; protein binding; protein homodimerization activity; protein heterodimerization activity; heat shock protein binding; metal ion binding; chaperone binding

Biological Process: response to fungus; regulation of protein heterodimerization activity; apoptosis; positive regulation of apoptosis; regulation of cell cycle; positive regulation of proteolysis; myeloid cell homeostasis; negative regulation of peptidyl-serine phosphorylation; B cell apoptosis; response to organic cyclic substance; negative regulation of cell proliferation; regulation of mitochondrial membrane potential; B cell homeostasis; response to gamma radiation; establishment and/or maintenance of transmembrane electrochemical gradient; B cell negative selection; aging; response to drug; organ regeneration; release of cytochrome c from mitochondria; response to mycotoxin; mitochondrial fusion; regulation of protein homodimerization activity; vagina development; endocrine pancreas development; limb morphogenesis; response to UV-C; cell proliferation; endoplasmic reticulum calcium ion homeostasis; response to ethanol; unfolded protein response, activation of signaling protein activity; response to hydrogen peroxide; DNA damage response, signal transduction resulting in induction of apoptosis; reduction of endoplasmic reticulum calcium ion concentration; blood vessel remodeling; caspase activation via cytochrome c; regulation of mitochondrial membrane permeability; brain development; post-embryonic camera-type eye morphogenesis