Mouse ApoA-IV Monoclonal Antibody | anti-ApoA-IV antibody

ApoA-IV antibody

Cat. Num. AAA830657

• Applications: ELISA, Western Blot
• Synonyms: ApoA-IV; Monoclonal Antibody; ApoA-IV antibody; Monoclonal ApoA-IV; Anti-ApoA-IV; Apolipoprotein A-Iv; ApoA4; MGC142156; Apolipoprotein A lV; MGC142154; Apo AIV; anti-ApoA-IV antibody

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG2a
• Clone Number: 1D4C11
• Form/Format: Supplied as a liquid in ascitic fluid containing 0.03% sodium azide.
• Concentration: 1.0 mg/ml (varies by lot)
• Sequence Length: 396

• Applicable Applications for anti-ApoA-IV antibody: ELISA (EIA), Western Blot (WB)
• Application Notes: ELISA: 1:10,000; WB: 1:500-1:2000
• Biological Significance: APOA4 is a 396-residue preprotein, which undergoes proteolytic processing to yield apo A-IV, a 376-reside mature O-linked glycoprotein. In most mammals, including humans, apo A-IV synthesis is confined to the intestine; however in mice and rats hepatic synthesis also occurs. Apo A-IV is secreted into circulation on the surface of newly synthesized chylomicron particles. Intestinal fat absorption dramatically increases the synthesis and secretion of apo A-IV, although its primary function in human lipid metabolism has not been established.
• Cross-Reactivity: Human
• Immunogen: ApoA-IV antibody was raised in Mouse using a purified recombinant fragment of APOA4 (aa21-396) expressed in E Coli as the immunogen.
• Preparation and Storage: Store at 4 degree C for short term storage. Store at -20 degree C for long term storage. Avoid repeated freeze/thaw cycles.

Western Blot

(Western Blot analysis using ApoA-IV antibodyWestern Blot showing APOA4 antibody used against human serum (1), human plasma (2), HepG2 cell lysate (3) and SMMC-7721 cell lysate (4).)

Related Product Information for anti-ApoA-IV antibody

Mouse monoclonal ApoA-IV antibody

Product Categories/Family for anti-ApoA-IV antibody

Nutrition & Metabolism

NCBI and Uniprot Product Information

• NCBI GI #: 71773110
• NCBI GeneID: 337
• NCBI Accession #: NP_000473.2
• NCBI GenBank Nucleotide #: NM_000482.3
• UniProt Accession #: P06727; Q14CW8; Q6Q787; A8MSL6
• Molecular Weight: 45,399 Da
• NCBI Official Full Name: apolipoprotein A-IV
• NCBI Official Synonym Full Names: apolipoprotein A-IV
• NCBI Official Symbol: APOA4
• NCBI Protein Information: apolipoprotein A-IV
• UniProt Protein Name: Apolipoprotein A-IV
• UniProt Gene Name: APOA4
• UniProt Synonym Gene Names: Apo-AIV; ApoA-IV
• UniProt Entry Name: APOA4_HUMAN

NCBI Description

Apoliprotein (apo) A-IV gene contains 3 exons separated by two introns. A sequence polymorphism has been identified in the 3'UTR of the third exon. The primary translation product is a 396-residue preprotein which after proteolytic processing is secreted its primary site of synthesis, the intestine, in association with chylomicron particles. Although its precise function is not known, apo A-IV is a potent activator of lecithin-cholesterol acyltransferase in vitro. [provided by RefSeq, Jul 2008]

Uniprot Description

APOA4: May have a role in chylomicrons and VLDL secretion and catabolism. Required for efficient activation of lipoprotein lipase by ApoC-II; potent activator of LCAT. Apoa-IV is a major component of HDL and chylomicrons. Synthesized primarily in the intestine and secreted in plasma. Belongs to the apolipoprotein A1/A4/E family.

Protein type: Secreted; Cell adhesion; Lipid-binding; Secreted, signal peptide; Endoplasmic reticulum

Chromosomal Location of Human Ortholog: 11q23

Cellular Component: extracellular space; chylomicron; cell surface; endoplasmic reticulum lumen; early endosome; extracellular region; cytosol

Molecular Function: lipid transporter activity; antioxidant activity; protein binding; protein homodimerization activity; copper ion binding; cholesterol transporter activity; cholesterol binding; phosphatidylcholine binding; lipid binding

Biological Process: positive regulation of fatty acid biosynthetic process; phototransduction, visible light; multicellular organismal lipid catabolic process; cholesterol metabolic process; removal of superoxide radicals; lipid homeostasis; neurite regeneration; protein-lipid complex assembly; lipoprotein metabolic process; cholesterol efflux; lipid transport; cholesterol biosynthetic process; phosphatidylcholine metabolic process; leukocyte adhesion; cholesterol homeostasis; reverse cholesterol transport; innate immune response in mucosa; phospholipid efflux; regulation of cholesterol absorption; response to lipid hydroperoxide; hydrogen peroxide catabolic process; positive regulation of lipoprotein lipase activity; retinoid metabolic process; regulation of cholesterol transport