Mouse APO-A1 (Apoliprotein A1) CLIA Kit | APO-A1 clia kit

Mouse APO-A1 (Apoliprotein A1) CLIA Kit

Cat. Num. AAA2532518

• Reactivity: Mouse
• Synonyms: APO-A1 (Apoliprotein A1); Mouse APO-A1 (Apoliprotein A1) CLIA Kit; APO-A1 clia kit

• Reactivity: Mouse
• Specificity: This kit recognizes natural and recombinant APO-A1. No significant cross-reactivity or interference between APO-A1 and analogues was observed.
• Sequence Length: 396

• Assay Type: Sandwich
• Detection Range: 1.563-100ng/mL
• Sensitivity: Min: 0.938ng/mL; Max: 100ng/mL

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for APO-A1 clia kit

Intended Uses: This CLIA kit applies to the in vitro quantitative determination of APO-A1 concentrations in serum, plasma and other biological fluids.

Principle of the Assay: This kit uses Sandwich-CLIA as the method. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to APO-A1. Standards or samples are added to the appropriate micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for APO-A1 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain APO-A1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured spectrophotometrically by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of APO-A1. You can calculate the concentration of APO-A1 in the samples by comparing the RLU value of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 71773110
• NCBI GeneID: 337
• NCBI Accession #: NP_000473.2
• NCBI GenBank Nucleotide #: NM_000482.3
• Molecular Weight: 45,399 Da
• NCBI Official Full Name: apolipoprotein A-IV
• NCBI Official Synonym Full Names: apolipoprotein A-IV
• NCBI Official Symbol: APOA4
• NCBI Protein Information: apolipoprotein A-IV
• UniProt Protein Name: Apolipoprotein A-IV
• UniProt Gene Name: APOA4
• UniProt Synonym Gene Names: Apo-AIV; ApoA-IV
• UniProt Entry Name: APOA4_HUMAN

NCBI Description

Apoliprotein (apo) A-IV gene contains 3 exons separated by two introns. A sequence polymorphism has been identified in the 3'UTR of the third exon. The primary translation product is a 396-residue preprotein which after proteolytic processing is secreted its primary site of synthesis, the intestine, in association with chylomicron particles. Although its precise function is not known, apo A-IV is a potent activator of lecithin-cholesterol acyltransferase in vitro. [provided by RefSeq, Jul 2008]

Uniprot Description

APOA4: May have a role in chylomicrons and VLDL secretion and catabolism. Required for efficient activation of lipoprotein lipase by ApoC-II; potent activator of LCAT. Apoa-IV is a major component of HDL and chylomicrons. Synthesized primarily in the intestine and secreted in plasma. Belongs to the apolipoprotein A1/A4/E family.

Protein type: Secreted; Lipid-binding; Cell adhesion; Secreted, signal peptide; Endoplasmic reticulum

Chromosomal Location of Human Ortholog: 11q23

Cellular Component: extracellular space; chylomicron; cell surface; endoplasmic reticulum lumen; early endosome; extracellular region; cytosol

Molecular Function: antioxidant activity; lipid transporter activity; protein binding; copper ion binding; protein homodimerization activity; cholesterol transporter activity; cholesterol binding; phosphatidylcholine binding; lipid binding

Biological Process: phototransduction, visible light; removal of superoxide radicals; lipid homeostasis; protein-lipid complex assembly; phosphatidylcholine metabolic process; leukocyte adhesion; regulation of cholesterol absorption; phospholipid efflux; hydrogen peroxide catabolic process; triacylglycerol catabolic process; retinoid metabolic process; multicellular organismal lipid catabolic process; positive regulation of fatty acid biosynthetic process; cholesterol metabolic process; neurite regeneration; cholesterol efflux; lipoprotein metabolic process; lipid transport; cholesterol biosynthetic process; cholesterol homeostasis; reverse cholesterol transport; innate immune response in mucosa; response to lipid hydroperoxide; positive regulation of lipoprotein lipase activity; regulation of cholesterol transport