Mouse anti-Human Apolipoprotein A4 / APOA4 Monoclonal Antibody | anti-APOA4 antibody

Mouse Monoclonal [clone 1D4C11] (IgG2a) to Human Apolipoprotein A4 / APOA4

Cat. Num. AAA245707

• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot, ELISA
• Purity: Ascites
• Synonyms: Apolipoprotein A4 / APOA4; Monoclonal Antibody; Mouse Monoclonal [clone 1D4C11] (IgG2a) to Human Apolipoprotein A4 / APOA4; Anti-Apolipoprotein A4 / APOA4 Antibody (aa21-396; clone 1D4C11) IHC-plus; APOA4; Apo-AIV; Apolipoprotein A-IV; Apolipoprotein A4; ApoA-IV; Human Apolipoprotein A4; anti-APOA4 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG2a
• Clone Number: 1D4C11
• Specificity: Human APOA4
• Purity/Purification: Ascites
• Form/Format: Ascites fluid, 0.03% sodium azide
• Sequence Length: 396

• Applicable Applications for anti-APOA4 antibody: Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)
• Application Notes: ELISA (1:10000), IHC-P (1:200 - 1:400), WB (1:500 - 1:2000); Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary anti...
• Target Species: Human
• Immunogen Description: Purified recombinant fragment of APOA4 (aa21-396) expressed in E Coli.
• Immunogen Type: Recombinant protein
• Immunogen: Apolipoprotein A4 / APOA4 antibody was raised against purified recombinant fragment of APOA4 (aa21-396) expressed in E Coli.
• Antigen Modification: aa21-396
• Preparation and Storage: Long term: -20 degree C; Short term: +4 degree C. Avoid repeat freeze-thaw cycles.

Immunohistochemistry (IHC)

(Anti-APOA4 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.)

Immunohistochemistry (IHC)

(Anti-APOA4 antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.)

Western Blot (WB)

(Western blot of anti-APOA4 monoclonal antibody against human serum (1), human plasma (2), HepG2 cell lysate (3) and SMMC-7721 cell lysate (4).)

Related Product Information for anti-APOA4 antibody

Apoliprotein (apo) A-IV gene contains 3 exons separated by two introns. A sequence polymorphism has been identified in the 3'UTR of the third exon. The primary translation product is a 396-residue preprotein which after proteolytic processing is secreted its primary site of synthesis, the intestine, in association with chylomicron particles. Although its precise function is not known, apo A-IV is a potent activator of lecithin-cholesterol acyltransferase in vitro.

NCBI and Uniprot Product Information

• NCBI GI #: 71773110
• NCBI GeneID: 337
• NCBI Accession #: NP_000473.2
• NCBI GenBank Nucleotide #: NM_000482.3
• UniProt Accession #: P06727; Q14CW8; Q6Q787; A8MSL6
• Molecular Weight: 45,399 Da
• NCBI Official Full Name: apolipoprotein A-IV
• NCBI Official Synonym Full Names: apolipoprotein A-IV
• NCBI Official Symbol: APOA4
• NCBI Protein Information: apolipoprotein A-IV; apo-AIV; apoA-IV; apolipoprotein A4
• UniProt Protein Name: Apolipoprotein A-IV
• Protein Family: Apolipoprotein
• UniProt Gene Name: APOA4
• UniProt Synonym Gene Names: Apo-AIV; ApoA-IV
• UniProt Entry Name: APOA4_HUMAN

NCBI Description

Apoliprotein (apo) A-IV gene contains 3 exons separated by two introns. A sequence polymorphism has been identified in the 3'UTR of the third exon. The primary translation product is a 396-residue preprotein which after proteolytic processing is secreted its primary site of synthesis, the intestine, in association with chylomicron particles. Although its precise function is not known, apo A-IV is a potent activator of lecithin-cholesterol acyltransferase in vitro. [provided by RefSeq, Jul 2008]

Uniprot Description

APOA4: May have a role in chylomicrons and VLDL secretion and catabolism. Required for efficient activation of lipoprotein lipase by ApoC-II; potent activator of LCAT. Apoa-IV is a major component of HDL and chylomicrons. Synthesized primarily in the intestine and secreted in plasma. Belongs to the apolipoprotein A1/A4/E family.

Protein type: Secreted, signal peptide; Secreted; Lipid-binding; Endoplasmic reticulum; Cell adhesion

Chromosomal Location of Human Ortholog: 11q23

Cellular Component: extracellular space; chylomicron; cell surface; endoplasmic reticulum lumen; early endosome; extracellular region; cytosol

Molecular Function: lipid transporter activity; antioxidant activity; protein binding; protein homodimerization activity; copper ion binding; cholesterol transporter activity; cholesterol binding; phosphatidylcholine binding; lipid binding

Biological Process: positive regulation of fatty acid biosynthetic process; phototransduction, visible light; cholesterol metabolic process; multicellular organismal lipid catabolic process; removal of superoxide radicals; neurite regeneration; lipid homeostasis; protein-lipid complex assembly; lipoprotein metabolic process; cholesterol efflux; lipid transport; cholesterol biosynthetic process; phosphatidylcholine metabolic process; leukocyte adhesion; cholesterol homeostasis; reverse cholesterol transport; innate immune response in mucosa; phospholipid efflux; regulation of cholesterol absorption; response to lipid hydroperoxide; hydrogen peroxide catabolic process; positive regulation of lipoprotein lipase activity; regulation of cholesterol transport; retinoid metabolic process