Mouse anti-Drosophila AMP Activated Protein Kinase alpha1 Monoclonal Antibody | anti-KINB2 antibody

AMP Activated Protein Kinase alpha1 (AMPK a1)

Cat. Num. AAA606638

• Reactivity: Drosophila
• Applications: ELISA, Western Blot, Immunoprecipitation, Immunohistochemistry, Immunofluorescence
• Purity: Affinity Purified; Purified by Protein G affinity chromatography.
• Synonyms: AMP Activated Protein Kinase alpha1; Monoclonal Antibody; AMP Activated Protein Kinase alpha1 (AMPK a1); Anti -AMP Activated Protein Kinase alpha1 (AMPK a1); anti-KINB2 antibody

• Host: Mouse
• Reactivity: Drosophila
• Clonality: Monoclonal
• Isotype: IgG2a
• Clone Number: 10B156
• Specificity: Recognizes both the alpha 1 and the alpha 2 isoforms of Drosophila AMP-activated protein kinase (AMPK). Species sequence homology: vertebrates, human, mouse, rat.
• Purity/Purification: Affinity Purified; Purified by Protein G affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide.

• Applicable Applications for anti-KINB2 antibody: ELISA (EL/EIA), Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: Suitable for use in ELISA, Immunofluorescence, Immunohistochemistry, Immunoprecipitation and Western Blot.; Dilution: Western Blot: 1:100-1:1,000. Detects a band of ~63kD in rat brain cell lysates.; Immunohistochemistry: Frozen sections
• Immunogen: Synthetic peptide corresponding to 29aa within the N-terminal catalytic domain of Drosophila AMPK alpha. Species Sequence Homology: Vertebrates, Human, Mouse AND Rat
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Testing Data

(Human brain tumor lysate probed with MBS606638.)

Related Product Information for anti-KINB2 antibody

AMPK is a highly conserved heterotrimeric protein kinase found in all eukaryotes. The heterotrimeric complex exists as multiple isoforms and consists of a catalytic alpha subunit, which is stabilised by non-catalytic regulatory beta- and gamma- subunits. AMPK plays a vital role in maintaining cellular energy homeostasis and is activated by rising levels of AMP under conditions of hypoxia, ischemia, exercise, low glucose levels.

Product Categories/Family for anti-KINB2 antibody

Antibodies; Abs to Protein Kinases

NCBI and Uniprot Product Information

• NCBI GI #: 6686784
• UniProt Accession #: Q9SCY5; O23481; F4JLS7
• Molecular Weight: 31,993 Da
• NCBI Official Full Name: AKIN beta2
• UniProt Protein Name: SNF1-related protein kinase regulatory subunit beta-2
• Protein Family: SNF1-related protein kinase regulatory
• UniProt Gene Name: KINB2
• UniProt Synonym Gene Names: AKIN subunit beta-2; AKINB2; AKINbeta2
• UniProt Entry Name: KINB2_ARATH

Uniprot Description

Function: Regulatory subunit of the probable trimeric SNF1-related protein kinase (SnRK) complex, which may play a role in a signal transduction cascade regulating gene expression and carbohydrate metabolism in higher plants. The SnRK complex may also be involved in the regulation of fatty acid synthesis by phosphorylation of acetyl-CoA carboxylase and in assimilation of nitrogen by phosphorylating nitrate reductase.

Subunit structure: Subunit of a probable heterotrimeric complex consisting of an alpha catalytic (KIN10 or KIN11) subunit, and a beta (KINB) and a gamma (KING or SNF4) non-catalytic regulatory subunits. Interacts with SNF4. Ref.1 Ref.8 Ref.9

Tissue specificity: Expressed in leaves, stems, roots, flower buds and flowers. Not detectable in siliques. Ref.1

Induction: Repressed in the dark. Ref.1

Domain: Kinase-interacting sequence (KIS) is specific for the alpha catalytic subunit interaction and Association with SNF1 Complex (ASC) is specific for the gamma non-catalytic regulatory subunit interaction. Ref.7

Sequence similarities: Belongs to the 5'-AMP-activated protein kinase beta subunit family.

Sequence caution: The sequence AEE83735.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.The sequence CAB10413.1 differs from that shown. Reason: Frameshift at positions 11, 19 and 27. The sequence CAB78678.1 differs from that shown. Reason: Frameshift at positions 11, 19 and 27.