Mouse AMP Activated Protein Kinase alpha1/2 Monoclonal Antibody | anti-Adra1b antibody

AMP Activated Protein Kinase alpha1/2 (AMPK a1/2)

Cat. Num. AAA604868

• Gene Names: Adra1b; [a]1b
• Reactivity: Drosophila, Human, Mouse, Rat
• Applications: ELISA, Western Blot, Immunoprecipitation, Immunohistochemistry, Immunofluorescence
• Purity: Affinity Purified; Purified by Protein G Chromatography.
• Synonyms: AMP Activated Protein Kinase alpha1/2; Monoclonal Antibody; AMP Activated Protein Kinase alpha1/2 (AMPK a1/2); Anti -AMP Activated Protein Kinase alpha1/2 (AMPK a1/2); anti-Adra1b antibody

• Host: Mouse
• Reactivity: Drosophila, Human, Mouse, Rat
• Clonality: Monoclonal
• Isotype: IgG2a
• Clone Number: 9E22
• Specificity: Recognizes both the alpha 1 and the alpha 2 isoforms of Drosophila AMP-activated protein kinase (AMPK). Species Crossreactivity: human, mouse, rat.
• Purity/Purification: Affinity Purified; Purified by Protein G Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide.

• Applicable Applications for anti-Adra1b antibody: ELISA (EL/EIA), Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: Suitable for use in Immunofluorescence, ELISA, Western Blot, Immunoprecipitation and Immunohistochemistry.; Dilution: Western Blot: 1:100-1:1000 Detects a band at ~63kD in rat brain cell lysates.; Immunohistochemistry: Frozen
• Immunogen: Synthetic peptide corresponding to 29aa within the N-terminal catalytic domain of Drosophila AMPK alpha.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Testing Data

(AMPK alpha 1/2 detected in human brain lysate using MBS604868.)

Related Product Information for anti-Adra1b antibody

AMPK is a highly conserved heterotrimeric protein kinase found in all eukaryotes. The heterotrimeric complex exists as multiple isoforms and consists of a catalytic alpha subunit, which is stabilized by non-catalytic regulatory beta- and gamma- subunits. AMPK plays a vital role in maintaining cellular energy homeostasis and is activated by rising levels of AMP under conditions of hypoxia, ischemia, exercise, low glucose levels.

Product Categories/Family for anti-Adra1b antibody

Antibodies; Abs to Protein Kinases

NCBI and Uniprot Product Information

• NCBI GI #: 548923936
• NCBI GeneID: 11548
• NCBI Accession #: NP_001271310.1
• NCBI GenBank Nucleotide #: NM_001284381.1
• UniProt Accession #: P97717
• Molecular Weight: 56,418 Da
• NCBI Official Full Name: alpha-1B adrenergic receptor
• NCBI Official Synonym Full Names: adrenergic receptor, alpha 1b
• NCBI Official Symbol: Adra1b
• NCBI Official Synonym Symbols: [a]1b
• NCBI Protein Information: alpha-1B adrenergic receptor; alpha-1B adrenoceptor; alpha-1B adrenoreceptor; alpha1B-adrenergic receptor
• UniProt Protein Name: Alpha-1B adrenergic receptor
• Protein Family: Alpha-1B adrenergic receptor
• UniProt Gene Name: Adra1b
• UniProt Synonym Gene Names: Alpha-1B adrenoceptor
• UniProt Entry Name: ADA1B_MOUSE

Uniprot Description

ADRA1B: a G protein-coupled catecholamine receptor that activates mitogenic responses and regulates growth and proliferation of many cells. There are 3 alpha-1-AR subtypes: alpha-1A, -1B and -1D, all of which signal through the Gq/11 family of G-proteins and different subtypes show different patterns of activation. Can induce neoplastic transformation when transfected into cell lines. Can regulate the phosphorylation status of the STAT3 pathway. Mediates blood pressure and aorta contractile responses induced by alpha-1 agonists. Activates a phosphatidylinositol second messenger system.

Protein type: GPCR, family 1; Receptor, GPCR; Membrane protein, multi-pass; Membrane protein, integral

Cellular Component: nuclear membrane; membrane; T-tubule; integral to membrane; plasma membrane; cytosol; nucleus

Molecular Function: G-protein coupled receptor activity; signal transducer activity; adrenoceptor activity; protein heterodimerization activity; alpha1-adrenergic receptor activity

Biological Process: adult heart development; vasoconstriction of artery involved in baroreceptor response to lowering of systemic arterial blood pressure; regulation of muscle contraction; positive regulation of the force of heart contraction by epinephrine-norepinephrine; response to amphetamine; response to hormone stimulus; response to morphine; locomotory behavior; signal transduction; glucose homeostasis; positive regulation of glycogen catabolic process; organ growth; G-protein coupled receptor protein signaling pathway; elevation of cytosolic calcium ion concentration; positive regulation of MAPKKK cascade; negative regulation of Rho protein signal transduction; behavioral response to cocaine; response to steroid hormone stimulus; regulation of vasoconstriction; visual learning; blood vessel remodeling; cell growth; positive regulation of heart rate by epinephrine-norepinephrine; negative regulation of glycogen catabolic process