Western Blot (WB)
(Western Blot analysis of PRKAA2 (arrow) (using rabbit pAb 293 cell lysates (2ug/lane) either nontransfected (Lane 1) or transiently transfected with MBS619057 which was the PRKAA1 gene (Lane 2))
Rabbit anti-Human, Mouse AMP Activated Protein Kinase alpha2 Polyclonal Antibody
AMP Activated Protein Kinase alpha2 (5'-AMP-activated Protein Kinase Catalytic Subunit alpha-2, AMPK alpha-2 Chain, AMPK Subunit alpha-2, AMPK a2, AMPK2, PRKAA2, PRKAA)
Reactivity
Human, Mouse
Applications
ELISA, Western Blot, Immunohistochemistry
Purity
Affinity PurifiedPurified by Protein A afinity chromatography.
Synonyms
AMP Activated Protein Kinase alpha2; Polyclonal Antibody; AMP Activated Protein Kinase alpha2 (5'-AMP-activated Protein Kinase Catalytic Subunit alpha-2; AMPK alpha-2 Chain; AMPK Subunit alpha-2; AMPK a2; AMPK2; PRKAA2; PRKAA); Anti -AMP Activated Protein Kinase alpha2 (5'-AMP-activated Protein Kinase Catalytic Subunit alpha-2; anti-AMP Activated Protein Kinase alpha2 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human PRKAA2. Species crossreactivity: mouse.
Purity/Purification
Affinity Purified
Purified by Protein A afinity chromatography.
Purified by Protein A afinity chromatography.
Form/Format
Supplied as a liquid in PBS, 0.09% sodium azide.
Applicable Applications for anti-AMP Activated Protein Kinase alpha2 antibody
ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
Suitable for use in ELISA, Western Blot and Immunohistochemistry.
Dilution: ELISA: 0.25ug/ml
Western Blot: 0.5-2.5ug/ml
Immunohistochemistry: 2.5-5ug/ml
Dilution: ELISA: 0.25ug/ml
Western Blot: 0.5-2.5ug/ml
Immunohistochemistry: 2.5-5ug/ml
Immunogen
Synthetic peptide corresponding to the C-terminal region of human PRKAA2 (KLH).
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(Western Blot analysis of PRKAA2 (arrow) (using rabbit pAb 293 cell lysates (2ug/lane) either nontransfected (Lane 1) or transiently transfected with MBS619057 which was the PRKAA1 gene (Lane 2))
Western Blot (WB)
(Western Blot analysis of PRKAA2 (arrow) (using rabbit pAb 293 cell lysates (2ug/lane) either nontransfected (Lane 1) or transiently transfected with MBS619057 which was the PRKAA1 gene (Lane 2))
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human skeletal muscle reacted with MBS619057, which was peroxidase conjugated to the secondary antibody, followed by DAB staining.)
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human skeletal muscle reacted with MBS619057, which was peroxidase conjugated to the secondary antibody, followed by DAB staining.)
Related Product Information for anti-AMP Activated Protein Kinase alpha2 antibody
The protein encoded by this gene is a catalytic subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta- methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. Studies of the mouse counterpart suggest that this catalytic subunit may control whole-body insulin sensitivity and is necessary for maintaining myocardial energy homeostasis during ischemia.
Product Categories/Family for anti-AMP Activated Protein Kinase alpha2 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI Official Full Name
AMP-activated protein kinase alpha2 subunit
Similar Products
Product Notes
The AMP Activated Protein Kinase alpha2 (Catalog #AAA619057) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The AMP Activated Protein Kinase alpha2 (5'-AMP-activated Protein Kinase Catalytic Subunit alpha-2, AMPK alpha-2 Chain, AMPK Subunit alpha-2, AMPK a2, AMPK2, PRKAA2, PRKAA) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's AMP Activated Protein Kinase alpha2 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC). Suitable for use in ELISA, Western Blot and Immunohistochemistry. Dilution: ELISA: 0.25ug/ml Western Blot: 0.5-2.5ug/ml Immunohistochemistry: 2.5-5ug/ml. Researchers should empirically determine the suitability of the AMP Activated Protein Kinase alpha2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "AMP Activated Protein Kinase alpha2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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