Western Blot (WB)
(Western blot analysis of alpha B Crystallin: Lane 1: MW marker, Lane 2: alpha-Crystallin, Lane 3: alpha A Crystallin, Lane 4: alpha B Crystallin, Lane 5: alpha-Crystallin.)
Mouse alpha B-Crystallin Monoclonal Antibody | anti-CRYAB; HSPB5 antibody
alpha B-Crystallin monoclonal antibody (1B6.1-3G4)
Reactivity
Human, Mouse, Rat, Bovine, Chicken
Applications
Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot
Purity
Protein G affinity purified.
Synonyms
alpha B-Crystallin; Monoclonal Antibody; alpha B-Crystallin monoclonal antibody (1B6.1-3G4); anti-CRYAB; HSPB5 antibody
Host
Mouse
Reactivity
Human, Mouse, Rat, Bovine, Chicken
Clonality
Monoclonal
Isotype
IgG1
Clone Number
1B6.1-3G4
Specificity
Recognizes human, mouse, rat, bovine and chicken alphaB-crystallin. Detects a band of ~20kDa by Western blot.
Purity/Purification
Protein G affinity purified.
Form/Format
Liquid. In PBS, pH 7.2, containing 50% glycerol and 0.09% sodium azide.
Sequence Length
175
Applicable Applications for anti-CRYAB; HSPB5 antibody
Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC) Paraffin, Western Blot (WB)
Application Notes
Immunohistochemistry (paraffin sections, 10ug/ml)
Western Blot (1:1,000, colorimetric)
Suggested dilutions/conditions may not be available for all applications.
Detects a band of ~20kDa by Western blot.
Western Blot (1:1,000, colorimetric)
Suggested dilutions/conditions may not be available for all applications.
Detects a band of ~20kDa by Western blot.
Immunogen
Bovine alpha B-crystallin.
Preparation and Storage
Long term storage: -20 degree C
Western Blot (WB)
(Western blot analysis of alpha B Crystallin: Lane 1: MW marker, Lane 2: alpha-Crystallin, Lane 3: alpha A Crystallin, Lane 4: alpha B Crystallin, Lane 5: alpha-Crystallin.)
Western Blot (WB)
(Western blot analysis of alpha B Crystallin: Lane 1: MW marker, Lane 2: alpha-Crystallin, Lane 3: alpha A Crystallin, Lane 4: alpha B Crystallin, Lane 5: alpha-Crystallin.)
Related Product Information for anti-CRYAB; HSPB5 antibody
Alpha-crystallins, which are part of the small Heat shock family members, are major water-soluble proteins present in the lens of the mammalian eye. Phosphorylation of serine residues which occurs during development and in response to stress, is intimately linked with its function. Chaperone activity requires, and is modulated by, oligomerization and is limited to binding unfolded intermediates to prevent irreversible aggregation.
Product Categories/Family for anti-CRYAB; HSPB5 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
20,037 Da
NCBI Official Full Name
Alpha-crystallin B chain
NCBI Official Symbol
CRYAB
NCBI Protein Information
alpha-crystallin B chain
UniProt Protein Name
Alpha-crystallin B chain
UniProt Gene Name
CRYAB
UniProt Synonym Gene Names
CRYA2
UniProt Entry Name
CRYAB_BOVIN
Uniprot Description
CRYAB: a major structural protein of the eye lens. A member of the small heat shock protein (sHSP also known as the HSP20) family. Alpha-B is expressed in the lens as well as other tissues. Elevated expression of alpha-B crystallin occurs in many neurological diseases; a missense mutation cosegregated in a family with a desmin-related myopathy.
Protein type: Heat shock protein; Chaperone
Cellular Component: cytoplasm; cytosol; mitochondrion; myelin sheath; nucleus; plasma membrane; Z disc
Molecular Function: metal ion binding; protein homodimerization activity; structural constituent of eye lens; unfolded protein binding
Biological Process: lens development in camera-type eye; muscle development; negative regulation of apoptosis; negative regulation of caspase activity; negative regulation of intracellular transport; protein homooligomerization; response to hydrogen peroxide; response to hypoxia; tubulin folding
Research Articles on CRYAB; HSPB5
Similar Products
Product Notes
The CRYAB; HSPB5 cryab (Catalog #AAA565003) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The alpha B-Crystallin monoclonal antibody (1B6.1-3G4) reacts with Human, Mouse, Rat, Bovine, Chicken and may cross-react with other species as described in the data sheet. AAA Biotech's alpha B-Crystallin can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC) Paraffin, Western Blot (WB). Immunohistochemistry (paraffin sections, 10ug/ml) Western Blot (1:1,000, colorimetric) Suggested dilutions/conditions may not be available for all applications. Detects a band of ~20kDa by Western blot. Researchers should empirically determine the suitability of the CRYAB; HSPB5 cryab for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "alpha B-Crystallin, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
