C-Type Natriuretic Peptide (CNP) Recombinant Protein | CNP recombinant protein

Recombinant C-Type Natriuretic Peptide (CNP)

Cat. Num. AAA2010082

• Gene Names: NPPC; CNP; CNP2
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: C-Type Natriuretic Peptide (CNP); Recombinant C-Type Natriuretic Peptide (CNP); CNP recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with N-terminal His-Tag, its sequence is listed below.; MGHHHHHHSGSEF- RTPPAEEL AEPQAAGGGQ KKGDKAPGGG GANLKGDRSR LLRDLRVDTK SRAAWARLLQ EHPNARKYKG ANKKGLSKGC FGLKLDRIGS MSGLG
• Sequence Length: 126

• Applicable Applications for CNP recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Homo sapiens (Human)
• Expression System: Prokaryotic expression
• Residues: Arg33~Gly125 (Accession # P23582) with N-terminal His-Tag
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 13249346
• NCBI GeneID: 4880
• NCBI Accession #: NP_077720.1
• NCBI GenBank Nucleotide #: NM_024409.2
• UniProt Accession #: P23582; Q4ZG41
• Molecular Weight: 11.3kDa
• NCBI Official Full Name: C-type natriuretic peptide
• NCBI Official Synonym Full Names: natriuretic peptide C
• NCBI Official Symbol: NPPC
• NCBI Official Synonym Symbols: CNP; CNP2
• NCBI Protein Information: C-type natriuretic peptide; natriuretic peptide precursor C
• UniProt Protein Name: C-type natriuretic peptide
• Protein Family: 2',3'-cyclic-nucleotide 3'-phosphodiesterase
• UniProt Gene Name: NPPC
• UniProt Synonym Gene Names: CNP2
• UniProt Entry Name: ANFC_HUMAN

NCBI Description

The protein encoded by this gene is proteolytically processed to form a secreted hormone of the natriuretic peptide family. The encoded hormone regulates the growth and differentiation of cartilaginous growth plate chondrocytes and may also be vasoactive and natriuretic. [provided by RefSeq, Sep 2011]

Uniprot Description

NPPC: Hormone which plays a role in endochondral ossification through regulation of cartilaginous growth plate chondrocytes proliferation and differentiation. May also be vasoactive and natriuretic. Specifically binds and stimulates the cGMP production of the NPR2 receptor. Binds the clearance receptor NPR3. Belongs to the natriuretic peptide family.

Protein type: Hormone; Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 2q37.1

Cellular Component: extracellular space; secretory granule

Molecular Function: protein homodimerization activity; peptide hormone receptor binding; hormone activity; receptor binding

Biological Process: response to drug; cGMP biosynthetic process; ossification; regulation of smooth muscle cell proliferation; regulation of cAMP metabolic process; regulation of multicellular organism growth; negative regulation of meiotic cell cycle; positive regulation of vasodilation; negative regulation of DNA metabolic process; post-embryonic development; negative regulation of cell proliferation; positive regulation of osteoblast differentiation; response to ethanol; positive regulation of cGMP biosynthetic process; receptor guanylyl cyclase signaling pathway; response to hypoxia; regulation of cGMP metabolic process; regulation of blood vessel size