Rabbit [pSer78]HSP27 Polyclonal Antibody | anti-HspB1; Heat shock protein 27 antibody

[pSer78]HSP27 polyclonal antibody

Cat. Num. AAA565319

• Gene Names: HSPB1; CMT2F; HMN2B; HSP27; HSP28; Hsp25; SRP27; HS.76067; HEL-S-102
• Reactivity: Human, Rat, Monkey, Bovine, Guinea pig
• Applications: Immunohistochemistry, Western Blot
• Purity: Protein A affinity purified.
• Synonyms: [pSer78]HSP27; Polyclonal Antibody; [pSer78]HSP27 polyclonal antibody; HSP27; anti-HspB1; Heat shock protein 27 antibody

• Host: Rabbit
• Reactivity: Human, Rat, Monkey, Bovine, Guinea pig
• Clonality: Polyclonal
• Specificity: Recognizes human, rat, bovine, guinea pig and monkey HSP27. Detects a band of ~27kDa by Western blot.
• Purity/Purification: Protein A affinity purified.
• Form/Format: Liquid. In PBS containing 50% glycerol and 0.09% sodium azide.
• Sequence Length: 205

• Applicable Applications for anti-HspB1; Heat shock protein 27 antibody: Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: Western Blot (1:1,000, colorimetric); Suggested dilutions/conditions may not be available for all applications.; Detects a band of ~27kDa by Western blot.
• Immunogen: Synthetic peptide corresponding to a portion of human HSP27 phosphorylated at Ser78.
• Preparation and Storage: Long term storage: -20 degree C; Avoid freeze/thaw cycles.

Western Blot (WB)

(Western blot analysis of HSP27 (pSer78), pAb: Lane 1 MW marker, Lane 2 phospho HSP27, Lane 3 non-phospho HSP27 and Lane 4 MDBK (weak reactivity).)

Related Product Information for anti-HspB1; Heat shock protein 27 antibody

Hsp27 is one of the most common members of the highly conserved and ubiquitously expressed family of small heat shock proteins (sHsp), which also includes alphaB-crystallin. It is characterized by a conserved C-terminal alpha-crystallin domain consisting of two anti-parallel beta-sheets that promote oligomer formation required for its primary chaperone function as inhibitor of irreversible protein aggregation. Hsp27 oligomerization is modulated by post-translational phosphorylation of Hsp27 at three serine residues, Ser15, Ser78, and Ser82, by a variety of protein kinases including MAPKAPK-3, PKAc-alpha, p70 S6K, PKD I, and PKC-delta. Hsp27 has been shown to inhibit actin polymerization by binding of unphosphorylated Hsp27 monomers to actin intermediate filaments. Anti-apoptotic functions of Hsp27 have also been identified through interactions with DAXX7, activation of Akt, and inhibition of apoptosome formation. Evidence suggests altered expression of Hsp27 is implicated in the pathogenesis of breast, ovarian, and prostate cancer.

Product Categories/Family for anti-HspB1; Heat shock protein 27 antibody

Protein Synthesis & Folding

NCBI and Uniprot Product Information

• NCBI GI #: 19855073
• NCBI GeneID: 3315
• UniProt Accession #: P04792; Q6FI47; Q96C20; Q96EI7; Q9UC31; Q9UC34; Q9UC35; Q9UC36; B2R4N8
• Molecular Weight: 22,783 Da
• NCBI Official Full Name: Heat shock protein beta-1
• NCBI Official Synonym Full Names: heat shock protein family B (small) member 1
• NCBI Official Symbol: HSPB1
• NCBI Official Synonym Symbols: CMT2F; HMN2B; HSP27; HSP28; Hsp25; SRP27; HS.76067; HEL-S-102
• NCBI Protein Information: heat shock protein beta-1
• UniProt Protein Name: Heat shock protein beta-1
• UniProt Gene Name: HSPB1
• UniProt Synonym Gene Names: HSP27; HSP28; HspB1; HSP 27; SRP27
• UniProt Entry Name: HSPB1_HUMAN

NCBI Description

The protein encoded by this gene is induced by environmental stress and developmental changes. The encoded protein is involved in stress resistance and actin organization and translocates from the cytoplasm to the nucleus upon stress induction. Defects in this gene are a cause of Charcot-Marie-Tooth disease type 2F (CMT2F) and distal hereditary motor neuropathy (dHMN). [provided by RefSeq, Oct 2008]

Uniprot Description

HSP27: a small heat shock protein that is regulated both transcriptionally and posttranslationally. Modulates actin polymerization and reorganization. Its expression level increases several-fold in response to stress and is phosphorylated by MAPKAP kinase 2. Cytoplasmic in interphase cells. Colocalizes with mitotic spindles in mitotic cells. Translocates to the nucleus during heat shock.

Protein type: Motility/polarity/chemotaxis; Chaperone; Heat shock protein

Chromosomal Location of Human Ortholog: 7q11.23

Cellular Component: cytoplasm; cytoskeleton; cytosol; extracellular space; focal adhesion; nucleus; plasma membrane; proteasome complex; spindle; Z disc

Molecular Function: identical protein binding; protein binding; protein kinase binding; protein kinase C binding; protein kinase C inhibitor activity; ubiquitin binding

Biological Process: cell motility; gene expression; negative regulation of apoptosis; negative regulation of protein kinase activity; positive regulation of angiogenesis; positive regulation of blood vessel endothelial cell migration; positive regulation of interleukin-1 beta production; positive regulation of tumor necrosis factor biosynthetic process; regulation of I-kappaB kinase/NF-kappaB cascade; regulation of mRNA stability; regulation of translational initiation; response to unfolded protein; response to virus; retinal homeostasis; vascular endothelial growth factor receptor signaling pathway

Disease: Charcot-marie-tooth Disease, Axonal, Type 2f; Neuronopathy, Distal Hereditary Motor, Type Iib