Rabbit alpha A/ alpha B-Crystallin Polyclonal Antibody | anti-CRYAA/CRYAB; HSPB4/HSPB5 antibody

alpha A/ alpha B-Crystallin polyclonal antibody

Cat. Num. AAA565257

• Gene Names: CRYAB; MFM2; CRYA2; CTPP2; HSPB5; CMD1II; CTRCT16; HEL-S-101
• Reactivity: Human, Mouse, Bovine
• Applications: Immunocytochemistry, Western Blot
• Purity: Protein A affinity purified.
• Synonyms: alpha A/ alpha B-Crystallin; Polyclonal Antibody; alpha A/ alpha B-Crystallin polyclonal antibody; anti-CRYAA/CRYAB; HSPB4/HSPB5 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Bovine
• Clonality: Polyclonal
• Specificity: Recognizes human, mouse and bovine alphaA/B-crystallin. Detects a band of ~20kDa by Western blot.
• Purity/Purification: Protein A affinity purified.
• Form/Format: Liquid. In PBS, pH 7.2, containing 0.09% sodium azide and 50% glycerol.
• Sequence Length: 175

• Applicable Applications for anti-CRYAA/CRYAB; HSPB4/HSPB5 antibody: Immunocytochemistry (ICC), Western Blot (WB)
• Application Notes: Western Blot (1:1,000, colorimetric); Suggested dilutions/conditions may not be available for all applications.; Detects a band of ~20kDa by Western blot.
• Immunogen: Synthetic peptide corresponding to the sequence near the C-terminus of human alpha A- and alpha B-Crystallin.
• Preparation and Storage: Long term storage: -20 degree C

Western Blot (WB)

(Western blot analysis: Lane 1: MWM, Lane 2:, Lane 3: Lane 4: HeLa HS, Lane 5: Mouse Heart.)

Related Product Information for anti-CRYAA/CRYAB; HSPB4/HSPB5 antibody

Alpha-crystallins composed of alpha A (~20 kDa) and alpha B (~20 kDa) subunits, are major water-soluble proteins accounting for almost 50% of total protein in the mammalian transparent eye lens and they are also found in a variety of other tissues. The two other crystallin families, beta and gamma, are homologous to each other but not to the alpha family or the sHsp's. Alpha-crystallins are also referred to as small heat shock proteins, since they are induced by increased temperature in a variety of organisms. The alpha-crystallins have sequence homology as well as structural and functional similarities with the small Hsp's such as Hsp25/27. Most small heat shock proteins have four common structural and functional features: (i) molecular weight between 12 and 43kDa; (ii) the formation of large oligomeric complexes composed of alphaA-crystallin, alphaB-crystallin and Hsp25/27; (iii) the moderately conserved alpha-crystallin domain in the central region of the protein; and (iv) molecular chaperone activity. The alpha-crystallin domain comprises approximately 90 residues, is bounded by variable N-terminal and C-terminal extensions and is involved in oligomer assembly. Oligomers can reach 800kDa or more and are dynamic, exhibiting subunit exchanges and organizational plasticity, possibly leading to functional diversity. Phosphorylation of serine residues occurs during development and in response to stress, and usually decreases oligomer size. Chaperone activity requires, and is modulated by, oligomerization and is limited to binding unfolded intermediates to prevent irreversible aggregation, although productive release and refolding of denatured proteins requires close cooperation with other chaperones. Other proposed functions include a role in membrane stabilization and modulation of intermediate filament organization during physiological stress and neurodegenerative disease.

Product Categories/Family for anti-CRYAA/CRYAB; HSPB4/HSPB5 antibody

Protein Synthesis & Folding

NCBI and Uniprot Product Information

• NCBI GI #: 117385
• NCBI GeneID: 1410
• UniProt Accession #: P02511; O43416; Q9UC37; Q9UC38; Q9UC39; Q9UC40; Q9UC41; B0YIX0
• Molecular Weight: 20,159 Da
• NCBI Official Full Name: Alpha-crystallin B chain
• NCBI Official Synonym Full Names: crystallin alpha B
• NCBI Official Symbol: CRYAB
• NCBI Official Synonym Symbols: MFM2; CRYA2; CTPP2; HSPB5; CMD1II; CTRCT16; HEL-S-101
• NCBI Protein Information: alpha-crystallin B chain
• UniProt Protein Name: Alpha-crystallin B chain
• UniProt Gene Name: CRYAB
• UniProt Synonym Gene Names: CRYA2; HSPB5; HspB5
• UniProt Entry Name: CRYAB_HUMAN

NCBI Description

Mammalian lens crystallins are divided into alpha, beta, and gamma families. Alpha crystallins are composed of two gene products: alpha-A and alpha-B, for acidic and basic, respectively. Alpha crystallins can be induced by heat shock and are members of the small heat shock protein (HSP20) family. They act as molecular chaperones although they do not renature proteins and release them in the fashion of a true chaperone; instead they hold them in large soluble aggregates. Post-translational modifications decrease the ability to chaperone. These heterogeneous aggregates consist of 30-40 subunits; the alpha-A and alpha-B subunits have a 3:1 ratio, respectively. Two additional functions of alpha crystallins are an autokinase activity and participation in the intracellular architecture. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. Alpha-A and alpha-B gene products are differentially expressed; alpha-A is preferentially restricted to the lens and alpha-B is expressed widely in many tissues and organs. Elevated expression of alpha-B crystallin occurs in many neurological diseases; a missense mutation cosegregated in a family with a desmin-related myopathy. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2014]

Uniprot Description

CRYAB: a major structural protein of the eye lens. A member of the small heat shock protein (sHSP also known as the HSP20) family. Alpha-B is expressed in the lens as well as other tissues. Elevated expression of alpha-B crystallin occurs in many neurological diseases; a missense mutation cosegregated in a family with a desmin-related myopathy.

Protein type: Heat shock protein; Chaperone

Chromosomal Location of Human Ortholog: 11q22.3-q23.1

Cellular Component: actin filament bundle; cell surface; cytoplasm; cytosol; Golgi apparatus; microtubule cytoskeleton; mitochondrion; myelin sheath; nucleoplasm; nucleus; plasma membrane; Z disc

Molecular Function: identical protein binding; metal ion binding; microtubule binding; protein binding; protein homodimerization activity; structural constituent of eye lens; unfolded protein binding

Biological Process: aging; lens development in camera-type eye; microtubule polymerization or depolymerization; muscle contraction; muscle development; negative regulation of apoptosis; negative regulation of caspase activity; negative regulation of cell growth; negative regulation of intracellular transport; protein folding; protein homooligomerization; response to estradiol stimulus; response to hydrogen peroxide; response to hypoxia; stress-activated MAPK cascade; tubulin folding

Disease: Cardiomyopathy, Dilated, 1ii; Cataract 16, Multiple Types; Myopathy, Myofibrillar, 2; Myopathy, Myofibrillar, Fatal Infantile Hypertonic, Alpha-b Crystallin-related