Rabbit anti-Mouse ADAM15  Monoclonal Antibody | anti-ADAM15  antibody

Rabbit anti Mouse ADAM15  Monoclonal Antibody

Cat. Num. AAA2544675

• Gene Names: ADAM15; MDC15
• Reactivity: Mouse
• Applications: ELISA
• Synonyms: ADAM15 ; Monoclonal Antibody; Rabbit anti Mouse ADAM15 Monoclonal Antibody; anti-ADAM15 antibody

• Host: Rabbit
• Reactivity: Mouse
• Clonality: Monoclonal
• Isotype: IgG
• Sequence Length: 797

• Applicable Applications for anti-ADAM15 antibody: ELISA (EIA)
• Application Notes: ELISA: 0.1-0.2 mug/mL. This antibody can be used at 0.1-0.2 mug/mL with the appropriate secondary reagents to detect Mouse ADAM15. The detection limit for Mouse ADAM15 is approximately 0.00245 ng/well.
• Immunogen: Recombinant Mouse ADAM15 protein
• Buffer: 0.2 mum filtered solution in PBS with 5% trehalose
• Preparation and Storage: This antibody can be stored at 2 degree C- 8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

Related Product Information for anti-ADAM15  antibody

ADAM15, also known as Metargidin, is a type I transmembrane glycoprotein belonging to the ADAM (A Disintegrin and Metalloprotease Domain) family of proteins and is widely expressed in different tissues and cell types. Members of this family contain an amino-terminal metalloprotease domain followed by a disintegrin domain, a cysteine-rich region and a membrane proximal EGF-like domain. The disintegrin domain of ADAM15/metargidin contains an RGD tripeptide sequence, suggesting that it may potentially interact with the integrin family of proteins. ADAM15 is a transmembrane multi-domain proteins implicated in proteolysis, cell-cell and cell-matrix interactions in various disease conditions. There is also evidence supporting a role for ADAM15 in angiogenesis and angioinvasion of tumor cells, which are critical for unrestrained tumor growth and metastatic spread. Given its diverse functions, ADAM15 may represent a pivotal regulatory component of tumor progression, an important target for therapeutic intervention, or emerge as a biomarker of disease progression.

NCBI and Uniprot Product Information

• NCBI GI #: 45602836
• NCBI GeneID: 8751
• Molecular Weight: 68,256 Da
• NCBI Official Full Name: ADAM15 isoform 8
• NCBI Official Synonym Full Names: ADAM metallopeptidase domain 15
• NCBI Official Symbol: ADAM15
• NCBI Official Synonym Symbols: MDC15
• NCBI Protein Information: disintegrin and metalloproteinase domain-containing protein 15
• UniProt Protein Name: Disintegrin and metalloproteinase domain-containing protein 15
• UniProt Gene Name: ADAM15
• UniProt Synonym Gene Names: MDC15; ADAM 15; MDC-15
• UniProt Entry Name: ADA15_HUMAN

NCBI Description

The protein encoded by this gene is a member of the ADAM (a disintegrin and metalloproteinase) protein family. ADAM family members are type I transmembrane glycoproteins known to be involved in cell adhesion and proteolytic ectodomain processing of cytokines and adhesion molecules. This protein contains multiple functional domains including a zinc-binding metalloprotease domain, a disintegrin-like domain, as well as a EGF-like domain. Through its disintegrin-like domain, this protein specifically interacts with the integrin beta chain, beta 3. It also interacts with Src family protein-tyrosine kinases in a phosphorylation-dependent manner, suggesting that this protein may function in cell-cell adhesion as well as in cellular signaling. Multiple alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq, Jul 2008]

Uniprot Description

ADAM15: Active metalloproteinase with gelatinolytic and collagenolytic activity. Plays a role in the wound healing process. Mediates both heterotypic intraepithelial cell/T-cell interactions and homotypic T-cell aggregation. Inhibits beta-1 integrin-mediated cell adhesion and migration of airway smooth muscle cells. Suppresses cell motility on or towards fibronectin possibly by driving alpha-v/beta-1 integrin (ITAGV-ITGB1) cell surface expression via ERK1/2 inactivation. Cleaves E-cadherin in response to growth factor deprivation. Plays a role in glomerular cell migration. Plays a role in pathological neovascularization. May play a role in cartilage remodeling. May be proteolytically processed, during sperm epididymal maturation and the acrosome reaction. May play a role in sperm-egg binding through its disintegrin domain. 11 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell adhesion; EC 3.4.24.-; Membrane protein, integral; Motility/polarity/chemotaxis; Protease

Chromosomal Location of Human Ortholog: 1q21.3

Cellular Component: cell surface; plasma membrane

Molecular Function: integrin binding; metalloendopeptidase activity; metallopeptidase activity; protein binding; SH3 domain binding

Biological Process: apoptosis; cell-matrix adhesion; extracellular matrix disassembly; innate immune response; integrin-mediated signaling pathway; negative regulation of cell growth; negative regulation of cell migration; negative regulation of cell-matrix adhesion