Mouse anti-Human ADAM15 Monoclonal Antibody | anti-ADAM15 antibody

Anti-ADAM15 Antibody, Mouse Monoclonal

Cat. Num. AAA8103094

• Gene Names: ADAM15; MDC15
• Reactivity: Human
• Applications: ELISA, Flow Cytometry, Functional Assay
• Purity: Protein A
• Synonyms: ADAM15; Monoclonal Antibody; Anti-ADAM15 Antibody; Mouse Monoclonal; ADAM15 Antibody; Mouse MAb; ADAM metallopeptidase domain 15; Anti-MDC15 Antibody; anti-ADAM15 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: Mouse IgG1
• Clone Number: 1B7G8
• Specificity: Human ADAM15.; No cross-reactivity in ELISA with Mouse ADAM9, Mouse ADAM15, Human cell lysate (293 cell line)
• Purity/Purification: Protein A
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-ADAM15 antibody: ELISA (EIA), Flow Cytometry (FC/FACS/FCM)
• Application Notes: ELISA: 1:1000-1:2000; FC/FACS/FCM: 1:25-1:100
• Immunogen: Recombinant Human ADAM15 protein
• Conjugation: Unconjugated
• Preparation: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human ADAM15 (rh ADAM15). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Flow Cytometry (FC/FACS)

(Flow cytometric analysis of human ADAM15 expression on HeLa cells. Cells were stained with purified anti-Human ADAM15, then a FITC-conjugated second step antibody. The histogram were derived from gated events with the forward and side light-scatter characteristics of intact cells.)

Related Product Information for anti-ADAM15 antibody

ADAM15, also known as Metargidin, is a type I transmembrane glycoprotein belonging to the ADAM (A Disintegrin and Metalloprotease Domain) family of proteins and is widely expressed in different tissues and cell types. Members of this family contain an amino-terminal metalloprotease domain followed by a disintegrin domain, a cysteine-rich region and a membrane proximal EGF-like domain. The disintegrin domain of ADAM15/metargidin contains an RGD tripeptide sequence, suggesting that it may potentially interact with the integrin family of proteins. ADAM15 is a transmembrane multi-domain proteins implicated in proteolysis, cell-cell and cell-matrix interactions in various disease conditions. There is also evidence supporting a role for ADAM15 in angiogenesis and angioinvasion of tumor cells, which are critical for unrestrained tumor growth and metastatic spread. Given its diverse functions, ADAM15 may represent a pivotal regulatory component of tumor progression, an important target for therapeutic intervention, or emerge as a biomarker of disease progression.

Product Categories/Family for anti-ADAM15 antibody

Notch Signaling

References

Poghosyan Z, et al. (2002) Phosphorylation-dependent interactions between ADAM15 cytoplasmic domain and Src family protein-tyrosine kinases. J Biol Chem. 277(7): 4999-5007.Carl-McGrath S, et al. (2005) The disintegrin-metalloproteinases ADAM9, ADAM12, and ADAM15 are upregulated in gastric cancer. Int J Oncol. 26(1): 17-24.Najy AJ, et al. (2008) ADAM15 supports prostate cancer metastasis by modulating tumor cell-endothelial cell interaction. Cancer Res. 68(4): 1092-9.Maretzky T, et al. (2009) Characterization of the catalytic activity of the membrane-anchored metalloproteinase ADAM15 in cell-based assays. Biochem J. 420(1): 105-13.

NCBI and Uniprot Product Information

• NCBI GI #: 46909592
• NCBI GeneID: 8751
• NCBI Accession #: NP_997074.1
• NCBI GenBank Nucleotide #: NM_207191.2
• UniProt Accession #: Q13444; Q13493; Q53XQ0; Q5SR68; Q5SR69; Q6R267; Q71S61; Q71S62; B3KQU5; B4DLB5; B4DMH8; E9PN65
• Molecular Weight: 68,256 Da
• NCBI Official Full Name: disintegrin and metalloproteinase domain-containing protein 15 isoform 1 preproprotein
• NCBI Official Synonym Full Names: ADAM metallopeptidase domain 15
• NCBI Official Symbol: ADAM15
• NCBI Official Synonym Symbols: MDC15
• NCBI Protein Information: disintegrin and metalloproteinase domain-containing protein 15
• UniProt Protein Name: Disintegrin and metalloproteinase domain-containing protein 15
• Protein Family: Disintegrin and metalloproteinase domain-containing protein
• UniProt Gene Name: ADAM15
• UniProt Synonym Gene Names: MDC15; ADAM 15; MDC-15
• UniProt Entry Name: ADA15_HUMAN

NCBI Description

The protein encoded by this gene is a member of the ADAM (a disintegrin and metalloproteinase) protein family. ADAM family members are type I transmembrane glycoproteins known to be involved in cell adhesion and proteolytic ectodomain processing of cytokines and adhesion molecules. This protein contains multiple functional domains including a zinc-binding metalloprotease domain, a disintegrin-like domain, as well as a EGF-like domain. Through its disintegrin-like domain, this protein specifically interacts with the integrin beta chain, beta 3. It also interacts with Src family protein-tyrosine kinases in a phosphorylation-dependent manner, suggesting that this protein may function in cell-cell adhesion as well as in cellular signaling. Multiple alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq, Jul 2008]

Uniprot Description

ADAM15: Active metalloproteinase with gelatinolytic and collagenolytic activity. Plays a role in the wound healing process. Mediates both heterotypic intraepithelial cell/T-cell interactions and homotypic T-cell aggregation. Inhibits beta-1 integrin-mediated cell adhesion and migration of airway smooth muscle cells. Suppresses cell motility on or towards fibronectin possibly by driving alpha-v/beta-1 integrin (ITAGV-ITGB1) cell surface expression via ERK1/2 inactivation. Cleaves E-cadherin in response to growth factor deprivation. Plays a role in glomerular cell migration. Plays a role in pathological neovascularization. May play a role in cartilage remodeling. May be proteolytically processed, during sperm epididymal maturation and the acrosome reaction. May play a role in sperm-egg binding through its disintegrin domain. 11 isoforms of the human protein are produced by alternative splicing.

Protein type: Protease; Membrane protein, integral; EC 3.4.24.-; Cell adhesion; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 1q21.3

Cellular Component: cell surface; plasma membrane

Molecular Function: integrin binding; metalloendopeptidase activity; metallopeptidase activity; protein binding; SH3 domain binding

Biological Process: apoptosis; cell-matrix adhesion; extracellular matrix disassembly; innate immune response; integrin-mediated signaling pathway; negative regulation of cell growth; negative regulation of cell migration; negative regulation of cell-matrix adhesion