ADAM15 / MDC15 recombinant protein

Recombinant Human ADAM15 / MDC15 Protein (His tag)

Cat. Num. AAA2546208

• Gene Names: ADAM15; MDC15
• Purity: > 97 % as determined by SDS-PAGE
• Synonyms: ADAM15 / MDC15; Recombinant Human ADAM15 / MDC15 Protein (His tag); ADAM15; RP11-540D14.1; MDC15; ADAM15 / MDC15 recombinant protein

• Host: Human Cells
• Purity/Purification: > 97 % as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH 7.4
• Sequence Length: 772

• Application Notes: The secreted recombinant human ADAM15 comprises 501 amino acids after proteolytic of the signal peptide and pro peptide and has a predicted molecular mass of 54 kDa. As a result of glycosylation, rh ADAM15 migrates as an approximately 65-70 kDa band in SDS-PAGE under reducing conditions.
• Predicted N Terminal: Asp 207
• Endotoxin: < 1.0 EU per mug of the protein as determined by the LAL method
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -70 degree C

SDS-PAGE

Related Product Information for ADAM15 / MDC15 recombinant protein

Background: ADAM15, also known as Metargidin, is a type I transmembrane glycoprotein belonging to the ADAM (A Disintegrin and Metalloprotease Domain) family of proteins and is widely expressed in different tissues and cell type s. Members of this family contain an amino-terminal metalloprotease domain followed by a disintegrin domain, a cysteine-rich region and a membrane proximal EGF-like domain. The disintegrin domain of ADAM15/metargidin contains an RGD tripeptide sequence, suggesting that it may potentially interact with the integrin family of proteins. ADAM15 is a transmembrane multi-domain proteins implicated in proteolysis, cell-cell and cell-matrix interactions in various disease conditions. There is also evidence supporting a role for ADAM15 in angiogenesis and angioinvasion of tumor cells, which are critical for unrestrained tumor growth and metastatic spread. Given its diverse functions, ADAM15 may represent a pivotal regulatory component of tumor progression, an important target for therapeutic intervention, or emerge as a biomarker of disease progression.

Description: A DNA sequence encoding the human ADAM15 (NP_997074.1) (Met 1-Thr 696) precursor with a C-terminal polyhistidine tag was expressed.

NCBI and Uniprot Product Information

• NCBI GI #: 46909592
• NCBI GeneID: 8751
• NCBI Accession #: NP_997074.1
• NCBI GenBank Nucleotide #: NM_207191.2
• UniProt Accession #: Q13444; Q13493; Q53XQ0; Q5SR68; Q5SR69; Q6R267; Q71S61; Q71S62; B3KQU5; B4DLB5; B4DMH8; E9PN65
• Molecular Weight: 68,256 Da
• NCBI Official Full Name: disintegrin and metalloproteinase domain-containing protein 15 isoform 1 preproprotein
• NCBI Official Synonym Full Names: ADAM metallopeptidase domain 15
• NCBI Official Symbol: ADAM15
• NCBI Official Synonym Symbols: MDC15
• NCBI Protein Information: disintegrin and metalloproteinase domain-containing protein 15
• UniProt Protein Name: Disintegrin and metalloproteinase domain-containing protein 15
• UniProt Gene Name: ADAM15
• UniProt Synonym Gene Names: MDC15; ADAM 15; MDC-15

NCBI Description

The protein encoded by this gene is a member of the ADAM (a disintegrin and metalloproteinase) protein family. ADAM family members are type I transmembrane glycoproteins known to be involved in cell adhesion and proteolytic ectodomain processing of cytokines and adhesion molecules. This protein contains multiple functional domains including a zinc-binding metalloprotease domain, a disintegrin-like domain, as well as a EGF-like domain. Through its disintegrin-like domain, this protein specifically interacts with the integrin beta chain, beta 3. It also interacts with Src family protein-tyrosine kinases in a phosphorylation-dependent manner, suggesting that this protein may function in cell-cell adhesion as well as in cellular signaling. Multiple alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq, Jul 2008]

Uniprot Description

ADAM15: Active metalloproteinase with gelatinolytic and collagenolytic activity. Plays a role in the wound healing process. Mediates both heterotypic intraepithelial cell/T-cell interactions and homotypic T-cell aggregation. Inhibits beta-1 integrin-mediated cell adhesion and migration of airway smooth muscle cells. Suppresses cell motility on or towards fibronectin possibly by driving alpha-v/beta-1 integrin (ITAGV-ITGB1) cell surface expression via ERK1/2 inactivation. Cleaves E-cadherin in response to growth factor deprivation. Plays a role in glomerular cell migration. Plays a role in pathological neovascularization. May play a role in cartilage remodeling. May be proteolytically processed, during sperm epididymal maturation and the acrosome reaction. May play a role in sperm-egg binding through its disintegrin domain. 11 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell adhesion; EC 3.4.24.-; Membrane protein, integral; Motility/polarity/chemotaxis; Protease

Chromosomal Location of Human Ortholog: 1q21.3

Cellular Component: cell surface; plasma membrane

Molecular Function: integrin binding; metalloendopeptidase activity; metallopeptidase activity; protein binding; SH3 domain binding

Biological Process: apoptosis; cell-matrix adhesion; extracellular matrix disassembly; innate immune response; integrin-mediated signaling pathway; negative regulation of cell growth; negative regulation of cell migration; negative regulation of cell-matrix adhesion