Rabbit anti-Human ADAM15 Polyclonal Antibody | anti-ADAM15 antibody

Anti-Human ADAM15 Antibody

Cat. Num. AAA415883

• Gene Names: ADAM15; MDC15
• Reactivity: Human
• Applications: ELISA, Western Blot
• Purity: Purified
• Synonyms: ADAM15; Polyclonal Antibody; Anti-Human ADAM15 Antibody; Anti-ADAM15 Antibody; anti-ADAM15 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified
• Form/Format: Liquid
• Concentration: 100ug/100ul (varies by lot)
• Sequence Length: 797

• Applicable Applications for anti-ADAM15 antibody: Peptide ELISA (EIA), Western Blot (WB)
• Application Notes: Recommended dilutions:; Peptide ELISA: 1:80,000; WB: 1:500 - 1:1000; Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
• Storage Buffer: PBS, pH 7.4 with 0.02% Sodium Azide
• Immunogen: Synthetic peptide conjugated to KLH
• Preparation and Storage: This product is stable for several weeks at 4 degree C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20 degree C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.

Western Blot (WB)

Related Product Information for anti-ADAM15 antibody

This gene is a member of the ADAM (a disintegrin and metalloproteinase) protein family. ADAM family members are type I transmembrane glycoproteins known to be involved in cell adhesion and proteolytic ectodomain processing of cytokines and adhesion molecules. This protein contains multiple functional domains including a zinc-binding metalloprotease domain, a disintegrin-like domain, as well as a EGF-like domain. Through its disintegrin-like domain, this protein specifically interacts with the integrin beta chain, beta 3. It also interacts with Src family protein-tyrosine kinases in a phosphorylation-dependent manner, suggesting that this protein may function in cell-cell adhesion as well as in cellular signaling. Multiple alternatively spliced transcript variants encoding distinct isoforms have been observed.

References

Kuefer,R., Day,K.C., Kleer,C.G., et al. ADAM15 disintegrin is associated with aggressive prostate and breast cancer disease. Neoplasia 8 (4), 319-329 (2006)

NCBI and Uniprot Product Information

• NCBI GI #: 45602836
• NCBI GeneID: 8751
• Molecular Weight: 68,256 Da
• NCBI Official Full Name: ADAM15 isoform 8
• NCBI Official Synonym Full Names: ADAM metallopeptidase domain 15
• NCBI Official Symbol: ADAM15
• NCBI Official Synonym Symbols: MDC15
• NCBI Protein Information: disintegrin and metalloproteinase domain-containing protein 15; MDC-15; a disintegrin and metalloproteinase domain 15 (metargidin); metalloprotease RGD disintegrin protein; metalloproteinase-like, disintegrin-like, and cysteine-rich protein 15
• UniProt Protein Name: Disintegrin and metalloproteinase domain-containing protein 15
• Protein Family: Disintegrin and metalloproteinase domain-containing protein
• UniProt Gene Name: ADAM15
• UniProt Entry Name: ADA15_HUMAN

NCBI Description

The protein encoded by this gene is a member of the ADAM (a disintegrin and metalloproteinase) protein family. ADAM family members are type I transmembrane glycoproteins known to be involved in cell adhesion and proteolytic ectodomain processing of cytokines and adhesion molecules. This protein contains multiple functional domains including a zinc-binding metalloprotease domain, a disintegrin-like domain, as well as a EGF-like domain. Through its disintegrin-like domain, this protein specifically interacts with the integrin beta chain, beta 3. It also interacts with Src family protein-tyrosine kinases in a phosphorylation-dependent manner, suggesting that this protein may function in cell-cell adhesion as well as in cellular signaling. Multiple alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq, Jul 2008]

Uniprot Description

ADAM15: Active metalloproteinase with gelatinolytic and collagenolytic activity. Plays a role in the wound healing process. Mediates both heterotypic intraepithelial cell/T-cell interactions and homotypic T-cell aggregation. Inhibits beta-1 integrin-mediated cell adhesion and migration of airway smooth muscle cells. Suppresses cell motility on or towards fibronectin possibly by driving alpha-v/beta-1 integrin (ITAGV-ITGB1) cell surface expression via ERK1/2 inactivation. Cleaves E-cadherin in response to growth factor deprivation. Plays a role in glomerular cell migration. Plays a role in pathological neovascularization. May play a role in cartilage remodeling. May be proteolytically processed, during sperm epididymal maturation and the acrosome reaction. May play a role in sperm-egg binding through its disintegrin domain. 11 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell adhesion; Motility/polarity/chemotaxis; Membrane protein, integral; Protease; EC 3.4.24.-

Chromosomal Location of Human Ortholog: 1q21.3

Cellular Component: adherens junction; cell projection; cell surface; acrosome; plasma membrane; integral to membrane

Molecular Function: integrin binding; protein binding; zinc ion binding; metallopeptidase activity; metalloendopeptidase activity; SH3 domain binding

Biological Process: integrin-mediated signaling pathway; extracellular matrix organization and biogenesis; apoptosis; cell-matrix adhesion; negative regulation of cell-matrix adhesion; proteolysis; extracellular matrix disassembly; collagen catabolic process; tissue regeneration; innate immune response; negative regulation of cell growth; angiogenesis; negative regulation of cell migration