Mouse anti-Human ACVR1B/ALK4 Monoclonal Antibody | anti-ACVR1B/ALK4 antibody

Mouse anti Human ACVR1B/ALK4 Monoclonal Antibody

Cat. Num. AAA2543428

• Gene Names: ACVR1B; ALK4; SKR2; ACTRIB; ACVRLK4
• Reactivity: Human
• Applications: ELISA
• Synonyms: ACVR1B/ALK4; Monoclonal Antibody; Mouse anti Human ACVR1B/ALK4 Monoclonal Antibody; anti-ACVR1B/ALK4 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1
• Sequence Length: 546

• Applicable Applications for anti-ACVR1B/ALK4 antibody: ELISA (EIA)
• Application Notes: ELISA: 0.5-1 mug/mL. This antibody can be used at 0.5-1 mug/mL with the appropriate secondary reagents to detect Human ALK4. The detection limit for Human ALK-4 is approximately 0.16 ng/well.
• Immunogen: Recombinant Human ALK4 protein
• Buffer: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human ALK4 extracellular domain (rh ALK4; NP_004293.1; Met 1-Glu 126). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2 degree C- 8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -70 degree C. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

NCBI and Uniprot Product Information

• NCBI GI #: 298231236
• NCBI GeneID: 91
• NCBI GenBank Nucleotide #: NM_020328.3
• Molecular Weight: 51,725 Da
• NCBI Official Full Name: activin receptor type-1B isoform c
• NCBI Official Synonym Full Names: activin A receptor type 1B
• NCBI Official Symbol: ACVR1B
• NCBI Official Synonym Symbols: ALK4; SKR2; ACTRIB; ACVRLK4
• NCBI Protein Information: activin receptor type-1B
• UniProt Protein Name: Activin receptor type-1B
• UniProt Gene Name: ACVR1B
• UniProt Synonym Gene Names: ACVRLK4; ALK4; ACTR-IB; ALK-4; SKR2
• UniProt Entry Name: ACV1B_HUMAN

NCBI Description

This gene encodes an activin A type IB receptor. Activins are dimeric growth and differentiation factors which belong to the transforming growth factor-beta (TGF-beta) superfamily of structurally related signaling proteins. Activins signal through a heteromeric complex of receptor serine kinases which include at least two type I and two type II receptors. This protein is a type I receptor which is essential for signaling. Mutations in this gene are associated with pituitary tumors. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Jun 2010]

Uniprot Description

ALK4: Transmembrane serine/threonine kinase activin type-1 receptor forming an activin receptor complex with activin receptor type-2 (ACVR2A or ACVR2B). Transduces the activin signal from the cell surface to the cytoplasm and is thus regulating a many physiological and pathological processes including neuronal differentiation and neuronal survival, hair follicle development and cycling, FSH production by the pituitary gland, wound healing, extracellular matrix production, immunosuppression and carcinogenesis. Activin is also thought to have a paracrine or autocrine role in follicular development in the ovary. Within the receptor complex, type-2 receptors (ACVR2A and/or ACVR2B) act as a primary activin receptors whereas the type-1 receptors like ACVR1B act as downstream transducers of activin signals. Activin binds to type-2 receptor at the plasma membrane and activates its serine- threonine kinase. The activated receptor type-2 then phosphorylates and activates the type-1 receptor such as ACVR1B. Once activated, the type-1 receptor binds and phosphorylates the SMAD proteins SMAD2 and SMAD3, on serine residues of the C- terminal tail. Soon after their association with the activin receptor and subsequent phosphorylation, SMAD2 and SMAD3 are released into the cytoplasm where they interact with the common partner SMAD4. This SMAD complex translocates into the nucleus where it mediates activin-induced transcription. Inhibitory SMAD7, which is recruited to ACVR1B through FKBP1A, can prevent the association of SMAD2 and SMAD3 with the activin receptor complex, thereby blocking the activin signal. Activin signal transduction is also antagonized by the binding to the receptor of inhibin-B via the IGSF1 inhibin coreceptor. ACVR1B also phosphorylates TDP2. ACVRIB is abundantly expressed in systemic sclerosis patient fibroblasts and production of collagen is also induced by activin-A/INHBA. This suggests that the activin/ACRV1B signaling mechanism is involved in systemic sclerosis. Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. TGFB receptor subfamily. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 2.7.11.30; Kinase, protein; Membrane protein, integral; Protein kinase, Ser/Thr (receptor); Protein kinase, TKL; STKR family; TKL group; Type1 subfamily

Chromosomal Location of Human Ortholog: 12q13

Cellular Component: activin receptor complex; cell surface; integral to plasma membrane; plasma membrane; receptor complex

Molecular Function: activin binding; activin receptor activity; activin receptor activity, type I; ATP binding; growth factor binding; protein binding; protein serine/threonine kinase activity; SMAD binding

Biological Process: activin receptor signaling pathway; G1/S transition of mitotic cell cycle; negative regulation of cell growth; peptidyl-threonine phosphorylation; positive regulation of activin receptor signaling pathway; positive regulation of erythrocyte differentiation; protein amino acid autophosphorylation; protein amino acid phosphorylation; regulation of transcription, DNA-dependent; signal transduction; transmembrane receptor protein serine/threonine kinase signaling pathway