Western Blot (WB)
(Sample: Recombinant S100B, Mouse.)
Rabbit S100 Calcium Binding Protein B (S100B) Polyclonal Antibody | anti-S100B antibody
Polyclonal Antibody to S100 Calcium Binding Protein B (S100B)
IHC: 5-20 ug/mL
ICC: 5-20 ug/mL
Optimal working dilutions must be determined by end user.
Avoid repeated freeze/thaw cycles.
Store at 4° for frequent use.
Aliquot and store at -20°C for 24 months.
Stability Test:
thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Western Blot (WB)
(Sample: Recombinant S100B, Mouse.)
Western Blot (WB)
(Sample: Recombinant S100B, Mouse.)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Mouse Kidney Tissue;Primary Ab: 20ug/ml Rabbit Anti-Mouse S100B AntibodySecond Ab: 2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: MBS2086047))
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Mouse Kidney Tissue;Primary Ab: 20ug/ml Rabbit Anti-Mouse S100B AntibodySecond Ab: 2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: MBS2086047))
NCBI and Uniprot Product Information
Uniprot Description
S100B: Weakly binds calcium but binds zinc very tightly- distinct binding sites with different affinities exist for both ions on each monomer. Physiological concentrations of potassium ion antagonize the binding of both divalent cations, especially affecting high-affinity calcium-binding sites. Binds to and initiates the activation of STK38 by releasing autoinhibitory intramolecular interactions within the kinase. Interaction with AGER after myocardial infarction may play a role in myocyte apoptosis by activating ERK1/2 and p53/TP53 signaling. Dimer of either two alpha chains, or two beta chains, or one alpha and one beta chain. The S100B dimer binds two molecules of STK38. Interacts with AGER. The S100B dimer interacts with two molecules of CAPZA1. Although predominant among the water-soluble brain proteins, S100 is also found in a variety of other tissues. Belongs to the S-101 family.
Protein type: Calcium-binding
Chromosomal Location of Human Ortholog: 10 C1|10 38.76 cM
Cellular Component: cell soma; cytoplasm; extracellular region; extracellular space; nucleus; perinuclear region of cytoplasm; ruffle
Molecular Function: calcium ion binding; calcium-dependent protein binding; identical protein binding; metal ion binding; protein homodimerization activity; RAGE receptor binding; receptor binding; zinc ion binding
Biological Process: cellular calcium ion homeostasis; energy reserve metabolic process; learning and/or memory; memory; positive regulation of apoptosis; positive regulation of cell proliferation; positive regulation of I-kappaB kinase/NF-kappaB signaling; positive regulation of myelination; positive regulation of synaptic transmission; regulation of cell shape; regulation of cytokine biosynthetic process; regulation of long-term neuronal synaptic plasticity; regulation of neuronal synaptic plasticity
Research Articles on S100B
Similar Products
Product Notes
The S100B s100b (Catalog #AAA2004439) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to S100 Calcium Binding Protein B (S100B) reacts with Mus musculus (Mouse) and may cross-react with other species as described in the data sheet. AAA Biotech's S100 Calcium Binding Protein B (S100B) can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB); Immunohistochemistry (IHC); Immunocytochemistry (ICC); Immunoprecipitation (IP). WB: 0.5-2 ug/mL IHC: 5-20 ug/mL ICC: 5-20 ug/mL Optimal working dilutions must be determined by end user. Researchers should empirically determine the suitability of the S100B s100b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "S100 Calcium Binding Protein B (S100B), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
