Immunohistochemistry (IHC)
(Formalin-fixed, paraffin-embedded human rectum stained with MBS602388 using peroxidase-conjugate and AEC chromogen. Note cytoplasmic staining of smooth muscles.)
Mouse Actin, Muscle Speci?c Monoclonal Antibody | anti-LOC100533357 antibody
Actin, Muscle Speci?c
Reactivity
Bovine, Canine, Equine, Human, Mouse, Porcine, Rat
Applications
Immunohistochemistry, Immunofluorescence
Purity
Affinity PurifiedPurified by Protein G affinity chromatography.
Synonyms
Actin; Muscle Speci?c; Monoclonal Antibody; Anti -Actin; Muscle Speci c; anti-LOC100533357 antibody
Host
Mouse
Reactivity
Bovine, Canine, Equine, Human, Mouse, Porcine, Rat
Clonality
Monoclonal
Isotype
IgG1,k
Clone Number
3F10
Specificity
Recognizes alpha-smooth muscle, alpha-skeletal and alpha-cardiac (sarcomeric) isoforms of human actin. Reacts with tumors arising from smooth muscle (leiomyosarcomas) and skeletal muscle (rhabdomyosarcomas). Species Crossreactivity: mouse, rat, porcine, equine, bovine and canine.
Purity/Purification
Affinity Purified
Purified by Protein G affinity chromatography.
Purified by Protein G affinity chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide. Also available without BSA and azide., See A0760-22AX.
Applicable Applications for anti-LOC100533357 antibody
Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes
Suitable for use in Immunofluorescence and Immunohistochemistry.
Dilution: Immunohistochemistry (Formalin/paraffin): 1-2ug/ml for 30 minutes at RT. No special pretreatment is required for immunohistochemical staining of formalin-fixed tissues.
Dilution: Immunohistochemistry (Formalin/paraffin): 1-2ug/ml for 30 minutes at RT. No special pretreatment is required for immunohistochemical staining of formalin-fixed tissues.
Immunogen
Purified actin. Cellular Localization: Cytoplasmic.
Positive Control
Muscle or sarcoma
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunohistochemistry (IHC)
(Formalin-fixed, paraffin-embedded human rectum stained with MBS602388 using peroxidase-conjugate and AEC chromogen. Note cytoplasmic staining of smooth muscles.)
Immunohistochemistry (IHC)
(Formalin-fixed, paraffin-embedded human rectum stained with MBS602388 using peroxidase-conjugate and AEC chromogen. Note cytoplasmic staining of smooth muscles.)
Related Product Information for anti-LOC100533357 antibody
Actin is a major component of the cytoskeleton. Actin can be resolved on the basis of its isoelectric points into three distinctive components: alpha, beta, and gamma in order of increasing isoelectric point. Anti-Muscle-Specific Actin recognizes alpha and gamma isotypes of all muscle groups. Non-muscle cells such as vascular endothelial cells and connective tissues are non-reactive. Also, neoplastic cells of non-muscle-derived tissue such as carcinomas, melanomas, and lymphomas are negative. This antibody is useful in the identification of rhabdoid cellular elements.
Product Categories/Family for anti-LOC100533357 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
41,766 Da
NCBI Official Full Name
actin, muscle
NCBI Official Symbol
LOC100533357
NCBI Protein Information
actin, muscle
UniProt Protein Name
Actin, muscle
UniProt Entry Name
ACTM_APLCA
Uniprot Description
Function: Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.Multiple isoforms are involved in various cellular functions such as cytoskeleton structure, cell mobility, chromosome movement and muscle contraction.
Subunit structure: Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of a two-stranded helix. Each actin can bind to 4 others.
Subcellular location: Cytoplasm › cytoskeleton.
Tissue specificity: Expressed in the muscular cells of the sheath surrounding abdominal ganglions.
Sequence similarities: Belongs to the actin family.
Similar Products
Product Notes
The LOC100533357 (Catalog #AAA602388) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Actin, Muscle Speci?c reacts with Bovine, Canine, Equine, Human, Mouse, Porcine, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Actin, Muscle Speci?c can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC), Immunofluorescence (IF). Suitable for use in Immunofluorescence and Immunohistochemistry. Dilution: Immunohistochemistry (Formalin/paraffin): 1-2ug/ml for 30 minutes at RT. No special pretreatment is required for immunohistochemical staining of formalin-fixed tissues. Researchers should empirically determine the suitability of the LOC100533357 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Actin, Muscle Speci?c, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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