SDS-PAGE (SDS)
(Lysates were prepared from mouse liver extract in reducing sample buffer, resolved by SDS-PAGE (30ug total protein/lane), and transferred to an Immobilon-P membrane. The blot was developed with 2ug/ml MBS609367 and chemiluminescent detection substrate.)
Rat anti-Mouse a1-Acid Glycoprotein Monoclonal Antibody | anti-Orm1 antibody
a1-Acid Glycoprotein (Alpha-1-acid Glycoprotein, AGP 1, AGP1, AGP-1, AGP-A, Alpha-1-AGP, Orosomucoid 1, Orosomucoid-1, OMD 1, OMD-1, ORM, ORM1)
Gene Names
Orm1; AGP; OMD; Orm; Agpa1
Reactivity
Mouse
Applications
Western Blot, Immunoprecipitation
Purity
Purified by protein G affinity chromatography from hybridoma culture supernatant
Synonyms
a1-Acid Glycoprotein; Monoclonal Antibody; a1-Acid Glycoprotein (Alpha-1-acid Glycoprotein; AGP 1; AGP1; AGP-1; AGP-A; Alpha-1-AGP; Orosomucoid 1; Orosomucoid-1; OMD 1; OMD-1; ORM; ORM1); Anti -a1-Acid Glycoprotein (Alpha-1-acid Glycoprotein; anti-Orm1 antibody
Host
Rat
Reactivity
Mouse
Clonality
Monoclonal
Isotype
IgG1
Clone Number
10K30
Specificity
Recognizes mouse a 1-acid Glycoprotein.
Purity/Purification
Purified by protein G affinity chromatography from hybridoma culture supernatant
Form/Format
Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 200ul sterile PBS.
Concentration
~0.5mg/ml (after reconsitution) (varies by lot)
Applicable Applications for anti-Orm1 antibody
Western Blot (WB), Immunoprecipitation (IP)
Application Notes
WB: 2ug/ml Mouse liver tissue
IP: 25ug/ml
Optimal dilutions to be determined by research.
IP: 25ug/ml
Optimal dilutions to be determined by research.
Immunogen
Mouse serum-derived a 1-acid Glycoprotein. Species sequence homology: 70% rat, 70% human.
Hybridoma
Fusion of a mouse myeloma with B cells obtained from a rat immunized with AGP purified from mouse serum.
Preparation and Storage
Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
SDS-PAGE (SDS)
(Lysates were prepared from mouse liver extract in reducing sample buffer, resolved by SDS-PAGE (30ug total protein/lane), and transferred to an Immobilon-P membrane. The blot was developed with 2ug/ml MBS609367 and chemiluminescent detection substrate.)
SDS-PAGE (SDS)
(Lysates were prepared from mouse liver extract in reducing sample buffer, resolved by SDS-PAGE (30ug total protein/lane), and transferred to an Immobilon-P membrane. The blot was developed with 2ug/ml MBS609367 and chemiluminescent detection substrate.)
Related Product Information for anti-Orm1 antibody
a1-Acid Glycoprotein (AGP; also OMD/Orosomucoid) is a 40-46kD member of the immunocalin subfamily, lipocalin family of molecules. In mouse, circulating AGP is principally the product of hepatocytes that originates from multiple related genes (AGP-1, -2 and -3 in Mus musculus). Circulating AGP-1 and -2 are both 189aa in length, the principal sources of protein, and show 83% aa identity; AGP-3 contributes little to the AGP pool. In mouse blood, AGP is normally 200-400ug/ml. In response to inflammatory mediators (IL-6; IL-1), its concentration will rise 2-10 fold. More importantly, a complex glycosylation pattern will also change, transitioning from modestly branched to highly branched oligosaccharides. This change is reflected in its bioactivity, which has been shown to be a function of carbohydrate branching. AGP is generally considered to be a suppressor of inflammation. Rat and human AGP share only 70% and 47% aa identity with mouse AGP, respectively.
Product Categories/Family for anti-Orm1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
alpha-1-acid glycoprotein
NCBI Official Synonym Full Names
orosomucoid 1
NCBI Official Symbol
Orm1
NCBI Official Synonym Symbols
AGP; OMD; Orm; Agpa1
NCBI Protein Information
alpha-1-acid glycoprotein
UniProt Protein Name
Alpha-1-acid glycoprotein
Protein Family
UniProt Gene Name
Orm1
UniProt Entry Name
A1AG_RAT
NCBI Description
an acute phase reactant protein; may have a role in the acute inflammation response [RGD]
Uniprot Description
ORM1: Functions as transport protein in the blood stream. Binds various ligands in the interior of its beta-barrel domain. Also binds synthetic drugs and influences their distribution and availability in the body. Appears to function in modulating the activity of the immune system during the acute-phase reaction. Belongs to the calycin superfamily. Lipocalin family.
Protein type: Secreted; Secreted, signal peptide
Cellular Component: extracellular space
Molecular Function: drug binding
Biological Process: response to drug; organ regeneration; response to vitamin E; regulation of immune system process; transport; acute-phase response; response to lipopolysaccharide; response to organic cyclic substance; chronic inflammatory response to antigenic stimulus; acute inflammatory response to antigenic stimulus
Research Articles on Orm1
1. Surprisingly, AGP-over-expressing mice were not protected against renal ischemia-reperfusion injury.
Similar Products
Product Notes
The Orm1 orm1 (Catalog #AAA609367) is an Antibody produced from Rat and is intended for research purposes only. The product is available for immediate purchase. The a1-Acid Glycoprotein (Alpha-1-acid Glycoprotein, AGP 1, AGP1, AGP-1, AGP-A, Alpha-1-AGP, Orosomucoid 1, Orosomucoid-1, OMD 1, OMD-1, ORM, ORM1) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's a1-Acid Glycoprotein can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunoprecipitation (IP). WB: 2ug/ml Mouse liver tissue IP: 25ug/ml Optimal dilutions to be determined by research. Researchers should empirically determine the suitability of the Orm1 orm1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "a1-Acid Glycoprotein, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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