CEACAM6/CD66c Active Protein | CEACAM6 active protein

Human CellExpCEACAM6 / CD66c, human recombinant

Cat. Num. AAA844163

• Gene Names: CEACAM6; NCA; CEAL; CD66c
• Applications: SDS-Page
• Purity: >=95%
• Synonyms: CEACAM6/CD66c; Human CellExpCEACAM6 / CD66c; human recombinant; CEACAM6; CD66c; CEAL; NCA; CEACAM6 active protein

• Host: HEK 293 cells
• Purity/Purification: >=95%
• Form/Format: Lyophilized from 0.22 mum filtered solution in PBS, pH 7.4. Normally Mannitol or Trehalose are added as protectants before lyophilization.; Appearance: Lyophilized
• Sequence Length: 344

• Applicable Applications for CEACAM6 active protein: SDS-PAGE
• Solubility/Reconstitution Instructions: Centrifuge the vial prior to opening. Reconstitute in PBS, pH 7.4. Do not vortex.
• Endotoxin Level: < 1.0 EU per mug of the rhCEACAM6 by the LAL method.
• Activity (Specifications/test method): Measured by the ability of the immobilized protein to support the adhesion of calcium ionophore treated human neutrophils. When 2 x 105 cells/well are added to CEACAM6 coated plates (10 mug/mL, 100 muL/well), 45-70% of the cells will adhere after 20 minutes at 37 degree C.
• Biological Activity: Measured by the ability of the immobilized protein to support the adhesion of calcium ionophore treated human neutrophils. When 2 x 105 cells/well are added to CEACAM6 coated plates (10 mug/mL, 100 muL/well), 45-70% of the cells will adhere after 20 minutes at 37 degree C.
• Results: Measured by the ability of the immobilized protein to support the adhesion of calcium ionophore treated human neutrophils. When 2 x 105 cells/well are added to CEACAM6 coated plates (10 mug/mL, 100 muL/well), 45-70% of the cells will adhere after 20 minutes at 37 degree C.
• Handling: Centrifuge the vial prior to opening.
• Preparation and Storage: At -20 degree C; Shelf Life: 12 months

Testing Data

Related Product Information for CEACAM6 active protein

Background: Carcinoembryonic antigen-related cell adhesion molecule 6 (non-specific cross reacting antigen) (CEACAM6) also known as CD66c (Cluster of Differentiation 66c), CEAL, NCA, and is one of seven human CEACAM family members within the immunoglobulin superfamily. In humans, CEACAMs include type I transmembrane proteins (CEACAM1, CEACAM3, and CEACAM4) and GPI-linked molecules (CEACAM5 through CEACAM8). There is no human CEACAM2. CEACAM 6 contains one N-terminal V-type Ig-like domain (N domain), followed by two C2-type Ig-like domains. It shows considerable glycosylation, including (sialyl) LewisX, which mediates binding to E-selectin, galectins and some bacterial fimbrae. CEACAM-6 is expressed by granulocytes and their progenitors. It is also expressed by epithelia of various organs and is upregulated in pancreatic and colon adenocarcinomas, as well as hyperplastic polyps. Resistance to adhesion-related apoptosis in tumor cells is conferred in the condition of CEACAM6 overexpression.

Product Categories/Family for CEACAM6 active protein

Cytokines; Growth Factors & Hormones; Human cell expressed recombinant proteinsCytokines; Growth Factors & Hormones; CDsCytokines; Growth Factors & Hormones; Cytokines & Growth Factors (A-Z) Proteins and Enzymes; Human cell expressed recombinant proteinsP

NCBI and Uniprot Product Information

• NCBI GI #: 612407822
• NCBI GeneID: 4680
• NCBI Accession #: NP_002474.4
• NCBI GenBank Nucleotide #: NM_002483.6
• UniProt Accession #: P40199; Q13774; Q14920; Q53XP7
• Molecular Weight: This protein rhCEACAM6 is fused with 6×his tag at C-terminus, has a calculated MW of 32 kDa expressed. The predicted N-terminus is Lys35. Protein migrates as 35-65 kDa in reduced SDS-PAGE resulting from glycosylation.
• NCBI Official Full Name: carcinoembryonic antigen-related cell adhesion molecule 6 preproprotein
• NCBI Official Synonym Full Names: carcinoembryonic antigen-related cell adhesion molecule 6 (non-specific cross reacting antigen)
• NCBI Official Symbol: CEACAM6
• NCBI Official Synonym Symbols: NCA; CEAL; CD66c
• NCBI Protein Information: carcinoembryonic antigen-related cell adhesion molecule 6
• UniProt Protein Name: Carcinoembryonic antigen-related cell adhesion molecule 6
• Protein Family: Carcinoembryonic antigen-related cell adhesion molecule
• UniProt Gene Name: CEACAM6
• UniProt Synonym Gene Names: NCA
• UniProt Entry Name: CEAM6_HUMAN

NCBI Description

This gene encodes a protein that belongs to the carcinoembryonic antigen (CEA) family whose members are glycosyl phosphatidyl inositol (GPI) anchored cell surface glycoproteins. Members of this family play a role in cell adhesion and are widely used as tumor markers in serum immunoassay determinations of carcinoma. This gene affects the sensitivity of tumor cells to adenovirus infection. The protein encoded by this gene acts as a receptor for adherent-invasive E. coli adhesion to the surface of ileal epithelial cells in patients with Crohn's disease. This gene is clustered with genes and pseudogenes of the cell adhesion molecules subgroup of the CEA family on chromosome 19. [provided by RefSeq, Apr 2014]

Uniprot Description

CEACAM6: Belongs to the immunoglobulin superfamily. CEA family

Protein type: Membrane protein, GPI anchor; Membrane protein, integral

Chromosomal Location of Human Ortholog: 19q13.2

Cellular Component: integral to plasma membrane; plasma membrane

Molecular Function: protein binding

Biological Process: cell-cell signaling; blood coagulation; signal transduction; leukocyte migration