Rat matrix metallopeptidase 10 (stromelysin 2) ELISA Kit | MMP10 elisa kit

Rat matrix metalloproteinase 10, MMP-10 ELISA Kit

Cat. Num. AAA701214

• Reactivity: Rat
• Synonyms: matrix metallopeptidase 10 (stromelysin 2); Rat matrix metalloproteinase 10; MMP-10 ELISA Kit; MMP-10 ELISA kit; SL-2; STMY2; matrix metalloprotease 10; matrix metalloproteinase 10; matrix metalloproteinase 10 (stromelysin 2) ; stromelysin 2; transin 2; MMP10 elisa kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of Rat MMP10. No significant cross-reactivity or interference between Rat MMP10 and analogues was observed.
• Sequence Length: 476

• Samples: Serum, plasma, tissue homogenates
• Detection Range: 80 pg/ml-2000 pg/ml
• Sensitivity: 50 pg/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%; Three samples of known concentration were tested twenty times on one plate to assess.; Inter-assay Precision (Precision between assays): CV% is less than 10%; Three samples of known concentration were tested in twenty assays to assess.
• Detection Wavelength: 450 nm
• Sample Volume: 50-100ul
• Protein Biological Process 1: Developmental Protein
• Protein Biological Process 3: Collagen degradation
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for MMP10 elisa kit

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with a goat-anti-rabbit antibody. Standards or samples are then added to the appropriate microtiter plate wells with a HRP-conjugated MMP-10 and antibody preparation specific for MMP-10 and incubated. Then substrate solutions are added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of MMP-10 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 19424154
• NCBI GeneID: 117061
• NCBI Accession #: NP_598198.1
• NCBI GenBank Nucleotide #: NM_133514.1
• UniProt Accession #: P07152
• Molecular Weight: 54,222 Da
• NCBI Official Full Name: stromelysin-2
• NCBI Official Synonym Full Names: matrix metallopeptidase 10 (stromelysin 2)
• NCBI Official Symbol: Mmp10
• NCBI Protein Information: stromelysin-2; SL-2; MMP-10; transin-2; stromelysin 2; matrix metalloproteinase 10; matrix metalloproteinase-10; transformation-associated protein 34A
• UniProt Protein Name: Stromelysin-2
• Protein Family: Stromelysin
• UniProt Gene Name: Mmp10
• UniProt Synonym Gene Names: SL-2; MMP-10
• UniProt Entry Name: MMP10_RAT

NCBI Description

enzyme and a transformation-associated protein; activates procollagenase and degrades fibronectin and some gelatins [RGD, Feb 2006]

Uniprot Description

Function: Can degrade fibronectin, gelatins of type I, III, IV, and V; weakly collagens III, IV, and V. Activates procollagenase.

Catalytic activity: Similar to stromelysin 1, but action on collagen types III, IV and V is weak.

Cofactor: Binds 2 zinc ions per subunit