Human matrix metallopeptidase 10 (stromelysin 2) ELISA Kit | MMP10 elisa kit

Human Matrix metalloproteinase 10, MMP-10 ELISA Kit

Cat. Num. AAA703295

• Gene Names: MMP10; SL-2; STMY2
• Reactivity: Human
• Synonyms: matrix metallopeptidase 10 (stromelysin 2); Human Matrix metalloproteinase 10; MMP-10 ELISA Kit; MMP-10 ELISA kit; SL-2; STMY2; matrix metalloprotease 10; matrix metalloproteinase 10; matrix metalloproteinase 10 (stromelysin 2) ; stromelysin 2; transin 2; MMP10 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of human MMP-10. No significant cross-reactivity or interference between human MMP-10 and analogues was observed.
• Sequence Length: 476

• Samples: Serum, plasma, tissue homogenates, cell lysates
• Assay Type: Quantitative Sandwich
• Detection Range: 78 pg/ml-5000 pg/ml
• Sensitivity: < 19.5 pg/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
• Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for MMP10 elisa kit

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for MMP-10 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MMP-10 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MMP-10 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MMP-10 bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 4505205
• NCBI GeneID: 4319
• NCBI Accession #: NP_002416.1
• NCBI GenBank Nucleotide #: NM_002425.2
• UniProt Accession #: P09238; Q53HH9; B2R9X9
• Molecular Weight: 54,151 Da
• NCBI Official Full Name: stromelysin-2 preproprotein
• NCBI Official Synonym Full Names: matrix metallopeptidase 10 (stromelysin 2)
• NCBI Official Symbol: MMP10
• NCBI Official Synonym Symbols: SL-2; STMY2
• NCBI Protein Information: stromelysin-2; MMP-10; transin 2; transin-2; stromelysin 2; matrix metalloprotease 10; matrix metalloproteinase-10; matrix metalloproteinase 10 (stromelysin 2)
• UniProt Protein Name: Stromelysin-2
• Protein Family: Stromelysin
• UniProt Gene Name: MMP10
• UniProt Synonym Gene Names: STMY2; SL-2; MMP-10
• UniProt Entry Name: MMP10_HUMAN

NCBI Description

Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. The enzyme encoded by this gene degrades proteoglycans and fibronectin. The gene is part of a cluster of MMP genes which localize to chromosome 11q22.3. [provided by RefSeq, Jul 2008]

Uniprot Description

Function: Can degrade fibronectin, gelatins of type I, III, IV, and V; weakly collagens III, IV, and V. Activates procollagenase.

Catalytic activity: Similar to stromelysin 1, but action on collagen types III, IV and V is weak.1 PublicationManual assertion based on experiment in:Ref.8

Cofactor: Binds 2 zinc ions per subunit.1 PublicationManual assertion based on experiment in:Ref.8