Typical Testing Data/Standard Curve (for reference only)
Sheep Stromelysin-2 ELISA Kit | ST2 elisa kit
Sheep Stromelysin-2 ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
INTENDED USE This ST2 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Sheep ST2. This ELISA kit for research use only!
PRINCIPLE OF THE ASSAY ST2 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-ST2 antibody and an ST2-HRP conjugate. The assay sample and buffer are incubated together with ST2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ST2 concentration since ST2 from samples and ST2-HRP conjugate compete for the anti-ST2 antibody binding site. Since the number of sites is limited, as more sites are occupied by ST2 from the sample, fewer sites are left to bind ST2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ST2 concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. The enzyme encoded by this gene degrades proteoglycans and fibronectin. The gene is part of a cluster of MMP genes which localize to chromosome 11q22.3. [provided by RefSeq, Jul 2008]
Uniprot Description
MMP10: Can degrade fibronectin, gelatins of type I, III, IV, and V; weakly collagens III, IV, and V. Activates procollagenase. Belongs to the peptidase M10A family.
Protein type: EC 3.4.24.22; Secreted; Extracellular matrix; Protease; Secreted, signal peptide
Chromosomal Location of Human Ortholog: 11q22.3
Cellular Component: extracellular space; proteinaceous extracellular matrix; extracellular region
Molecular Function: zinc ion binding; metalloendopeptidase activity; calcium ion binding
Biological Process: collagen catabolic process; extracellular matrix disassembly; extracellular matrix organization and biogenesis; proteolysis; regulation of cell migration
Research Articles on ST2
Similar Products
Product Notes
The Sheep ST2 mmp10 (Catalog #AAA738472) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA738472 ELISA Kit recognizes Sheep ST2. It is sometimes possible for the material contained within the vial of "Stromelysin-2, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
