SDS-PAGE
(3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain)
MIEN1 recombinant protein
MIEN1, 1-112aa, Human, His tag, E Coli
Gene Names
MIEN1; C35; ORB3; XTP4; RDX12; C17orf37
Applications
SDS-Page
Purity
> 95% by SDS-PAGE
Synonyms
MIEN1; 1-112aa; Human; His tag; E Coli; Migration and invasion enhancer 1; C17orf37; C35; ORB3; RDX12; XTP4; MIEN1 recombinant protein
Host
E Coli
Purity/Purification
> 95% by SDS-PAGE
Form/Format
Liquid. In 20mM Tris-HCl Buffer (pH 8.0) containing 0.15M NaCl, 10% glycerol, 1mM DTT
Concentration
0.5 mg/ml (determined by Bradford assay) (varies by lot)
Sequence Positions
1-112
Sequence
MGSSHHHHHH SSGLVPRGSH MGSMSGEPGQ TSVAPPPEEV EPGSGVRIVV EYCEPCGFEA TYLELASAVK EQYPGIEIES RLGGTGAFEI EINGQLVFSK LENGGFPYEK DLIEAIRRAS NGETLEKITN SRPPC
Sequence Length
112
Applicable Applications for MIEN1 recombinant protein
SDS-PAGE
Antigen Species
Human
Tag Information
His-tag
Preparation and Storage
Can be stored at 2 degree C to 8 degree C for short term (1-2 weeks).
For long term storage, aliquot and store at -20 degree C to -80 degree C.
Avoid repeated freezing and thawing cycles.
For long term storage, aliquot and store at -20 degree C to -80 degree C.
Avoid repeated freezing and thawing cycles.
SDS-PAGE
(3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain)
SDS-PAGE
(3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain)
Related Product Information for MIEN1 recombinant protein
MIEN1 belongs to the SelWTH family. This protein Increases cell migration by inducing filopodia formation at the leading edge of migrating cells and plays a role in regulation of apoptosis, possibly through control of CASP3. It also may be involved in a redox-related process. Recombinant human MIEN1 protein, fused to His-tag at N-terminus, was expressed in E Coli and purified by using conventional chromatography techniques.
Product Categories/Family for MIEN1 recombinant protein
References
Dasgupta,S., et al. (2011) J. Biol. Chem. 286 (29), 25935-25946; Katz,E., et al. (2011) Anal Cell Pathol (Amst) 34 (1-2), 35-48
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
14.5kDa (135aa), confirmed by MALDI-TOF
NCBI Official Full Name
migration and invasion enhancer 1 isoform 1
NCBI Official Synonym Full Names
migration and invasion enhancer 1
NCBI Official Symbol
MIEN1
NCBI Official Synonym Symbols
C35; ORB3; XTP4; RDX12; C17orf37
NCBI Protein Information
migration and invasion enhancer 1
UniProt Protein Name
Migration and invasion enhancer 1
Protein Family
UniProt Gene Name
MIEN1
UniProt Synonym Gene Names
C17orf37; RDX12; XTP4
Uniprot Description
Increases cell migration by inducing filopodia formation at the leading edge of migrating cells. Plays a role in regulation of apoptosis, possibly through control of CASP3. May be involved in a redox-related process.
Research Articles on MIEN1
Similar Products
Product Notes
The MIEN1 mien1 (Catalog #AAA205268) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 1-112 with tag His-tag. AAA Biotech's MIEN1 can be used in a range of immunoassay formats including, but not limited to, SDS-PAGE. Researchers should empirically determine the suitability of the MIEN1 mien1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: MGSSHHHHHH SSGLVPRGSH MGSMSGEPGQ TSVAPPPEEV EPGSGVRIVV EYCEPCGFEA TYLELASAVK EQYPGIEIES RLGGTGAFEI EINGQLVFSK LENGGFPYEK DLIEAIRRAS NGETLEKITN SRPPC. It is sometimes possible for the material contained within the vial of "MIEN1, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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